Ipo11 (BC031900) Mouse Untagged Clone

CAT#: MC220849

Ipo11 (untagged) - Mouse importin 11 (cDNA clone MGC:39010 IMAGE:5364208), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ipo11
• Synonyms: 1700081H05Rik, 2510001A17Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC031900
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCTCAATAGTGCCAGCTCTGTGGTTCTTCAGGTCCTAACACAGGCCACCAGTCAGGATACTGCTG
  TGTTGAAGCCAGCCGAGGAGCAGCTGAAACAGTGGGAGACACAGCCAGGTTTCTATTCAGTGTTGCTGAA
  TATTTTTACGAATCACACCCTTGATATAAATGTACGGTGGCTTGCTGTGCTGTATTTTAAACATGGGATT
  GATCGTTACTGGAGGCGTGTCGCACCGCATGCTCTCTCAGAAGAGGAAAAGTCAACATTGCGTGCAGGGC
  TCATCACCAACTTTAATGAACCAATAAACCAGATTGCGACCCAGATCGCAGTGCTGATTGCCAAGGTTGC
  TCGGTTAGACTGTCCTAGGCAGTGGCCGGAGCTCATTCCCACTCTTGTAGAGTCTGTCAAAGTCCAGGAT
  GACCTTCGCCAGCACAGAGCTCTGCTGACCTTCTACCATGTCACCAAGACGCTGGCATCCAAACGCCTGG
  CCGCTGACAGAAAGCTATTCTATGATTTAGCTTCTGGAATTTATAATTTTGCCTGTTCTCTGTGGAACCA
  TCATACAGACACATTTCTGCAGCACGTTTCTTCTGGCAATGAAGCTGCGGTTCTGAGTTCACTAGAGCGA
  ACACTGCTATCGTTAAAAGTGCTGCGGAAGTTAACTGTGAATGGATTTGTGGAACCTCATAAGAATATGG
  AGGTGATGGGTTTTTTACATGGAATATTTGAACGTCTAAAACAGTTTCTGGAGTGCAGCAGAAGTATAGG
  TACAGATAATGTGTGTCGAGATAGACTGGAAAAGACCATCATTCTTTTTACTAAAGTGCTCTTGGACTTC
  TTGGATCAACACCCCATTTCATTTACTCCTCTAATTCAGAGGTCACTGGAATTTTCTGTAAGCTATGTTT
  TTACTGAAGTTGGTGAAGGCGTTACATTTGAGCGATTCATTGTCCAGTGCATGAATCTTATTAAGATGAT
  TGTCAAAAATTATGCTTATAAGCCATCCAAAAATTTTGAAGATAGCAGCCCTGAAACACTTGAAGCCCAT
  AAGATTAAGATGGCATTCTTCACATATCCCACGTTGACAGAGATATGTCGACGATTAGTCTCTCATTATT
  TTTTATTAACGGAAGAAGAACTGACCATGTGGGAGGAAGATCCAGAAGGCTTTACAGTGGAAGAAACAGG
  AGGAGACTCTTGGAAATACAGTTTGAGGCCTTGCACTGAAGTATTATTTATAGATATATTCCACGAATAT
  AATCAAACTCTTACTCCTGTACTTCTAGAAATGATGCAAACTCTTGAAGGTCCAACCAATGTAGAAGATA
  TGAATGCACTGTTAATCAAAGATGCTGTGTATAATGCTGTGGGATTAGCTGCTTTTGAGCTGTTTGACAG
  TGTTGATTTTGATCAGTGGTTTAAAACCCAACTTCTTCCCGAATTACAAGTTTCTCATAATAGGTATAAG
  CCATTACGACGCAGGGTGATTTGGCTCATTGGTCAGTGGATTTCTGTGAAATTCAAGTCTGACTTGAGAC
  CCATGCTGTATGAAGCAATCTGTAACTTGCTTCAAGATCAGGATTTAGTGGTCCGAATTGAAACAGCAAC
  AACTCTGAAGTTAACTGTTGATGATTTTGAATTTAGGACAGATCAGTTTTTACCGTATCTGGAAACCATG
  TTCACACTGCTTTTCCAGTTGCTGCAGCAGGTTACAGAGTGTGATACAAAGATGCACGTGCTGCACGTCC
  TCTCCTGTGTGATCGAAAGAGTCAACGTGCAGATCCGACCATATGTGGGGTGTTTGGTACAGTATTTGCC
  TCTTCTCTGGAAGCAGAGCGAAGAACACAACATGCTGCGATGCGCTATTCTGACAACACTTATCCACCTT
  GTTCAGGGATTAGGAGCAGACAGCAAGAACCTGTACCCTTTCCTGCTCCCAGTTATCCAGCTGAGTACAG
  ATGTGTCTCAGCCTCCACATGTCTATCTTCTGGAAGATGGCCTGGAGTTATGGTTAGTGACATTGGAGAA
  CAGCCCATGTGTCACACCAGAGCTGCTTCGTATATTTCAGAACATGTCACCTCTTCTTGAGCTTCCATCC
  AGCTGCCTTGCAAACGAATTAAGTTCAGAGAATCTCAGAACCTGCTTTAAGATCATCAATGGCTATATCT
  TTTTATCATCAACAGAATTTTTACAGACATATGCAGCAGGTTTGTGCCAGTCCTTTTATGAGCTGCTACC
  AGAGATTACTACAGAAGGTCAAGTTCAAGTGCTGAAGGTTGTGGAAAATGCCCTTAAAGTGAATCCAGTA
  TTAGGCCCACAGATGTTTCAGCGAATTCTACCCTGTGTTTTCAGAGGTGTTATTGAAGGCGAGAGGTATC
  CAGTAGTGATGTCCATTTATCTTGCTGTCATGGGTCGAGTCTTACTCCAGAATACCAGCTTCTTTTCTTC
  ACTTCTTAATGAGATGGGACACGAGTTTAATCAGGAGATGGACCAGCTTTTAGGAAATGTGATTGAAATG
  TGGGTCGATCGAATGGACAACATCACACAGCCCGAAAGAAAGAAACTCTCAGCTTTGGCTTTGCTTTCTC
  TTCTGCCTTCTGATAATAGTGTTATCCAAGATAAATTTTGTGGAATTATAAATATCTCAGTGGAGGCTCT
  TCATGATGTCATGACAGAAGACCCTGAAACAAGAACTTACAAAGATTGTATGTTGATGTCTCAACACGAG
  GAACCGAAAGTAACAGAAGATGAAGAGCCACCCACAGAACAAGACAAGAGGAAAAAGATGCTGGCCCTGA
  AGGACCCGGTGCACACAGTGTCTCTGCAGCAGTTCATCTATGAGAAGCTCAAGGCGCAGCAGGAGATCCT
  GGGGGAGCAAGGCTTCCAGTCCCTCATGGAAACGGTGGACACAGAGATTGTCACCCAGCTGCAGGAGTTT
  TTGCAAGGATTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC031900
• Insert Size: 2955 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC031900, AAH31900
• RefSeq Size: 4259 bp
• RefSeq ORF: 2954 bp
• Locus ID: 76582
• Cytogenetics: 13 D1- D2.1
• Gene Summary: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of RPL12, and of UBE2E3 (By similarity).[UniProtKB/Swiss-Prot Function]