Osbpl3 (BC086485) Mouse Untagged Clone

CAT#: MC220828

Osbpl3 (untagged) - Mouse oxysterol binding protein-like 3 (cDNA clone MGC:102333 IMAGE:5344992), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Osbpl3
• Synonyms: ORP3, OSBP3, A530055M08
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC086485
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGACGAGAAGAATCTCGGTGTGTCTCAAAAGTTGGTTTCACCTTCAAGAAGCACAAGTAGTTGCT
  CCTCCAAGCAAGGAAGCCGGCAGGACAGCTGGGAAGTCGTGGAAGGATTGAGAGGGGAAATGACTTACAC
  CCAGGAGCCGCCAGTGCAGAAAGGATTTTTGCTGAAAAAGAGGAAGTGGCCCTTAAAAGGCTGGCACAAG
  AGATTCTTCTGCCTGGAAAAGGGAATCTTGAAATATGCCAAGAGCCAAGCTGATATTGAGCGGGAGAAGT
  TGCATGGCTGCATTGACGTGGGGCTCTCTGTGATGTCTGTGAAGAAGTCGTCTAAGTGTATCGACCTAGA
  CACGGAGGAGCACATCTACCACCTGAAGGTGAAGTCAGAAGAACTCTTTGACGAGTGGGTGTCCAAGCTC
  CGTCACCACAGAATGTACCGGCAGAATGAGATCGCCATGTTTCCCCGGGATGTCAATCACTTTTTCTCAG
  GGTCCTCTGTCACAGATTCTGCCCCAGGAGTGTTCGAGTCAGTTTCCAGTAGGAAGCGTAGCAGTCTATC
  AAAGCAGAATTCATTTCCACCCGGAAGCAATTTGTCATTTTCTTGTGGTGGCGACACTCGAGTTCCTTTC
  TGGCTACAGTCTTCAGAGGACATGGAAAAATGTTCAAAAGACCTGGCACACTGCCATGCCTACCTGCTGG
  AAATGAGCCAGCTCTTAGAAAGCATGGACGTCCTGCATCGGACATACTCGGCGCCAGCCATCAACGCCAT
  CCAGGTCCCTAAGCCTTTTTCTGGCCCTGTGAGACTGCACTCCTCCAATCCTAACTTGTCGACGCTGGAC
  TTTGGAGAAGAGAAGTCTTACTCGGATGGTTCTGAAGCCTCGTCGGAGTTCTCCAAGATGCAGGAGGATC
  TGTGCCATGTCGCCCACAAAGTTTACTTCGCTTTAAGGTCGGCTTTCAATAGCATATCGGTGGAGAGAGA
  GAAACTGAAGCAGCTGATGGAGCTGGACACCTCCCCCTCCCCCTCCGCTCAGGTCGTCGGGCTGAAGCAC
  GCTCTGTCATCCGAAAACTCCAGGGATGAGGGCCGAGCCCTGGTGCACCAGCTCTCCAATGAGAGCAGGC
  TGTCTATCACCGACTCCCTTTCTGAGTTCTTCGATGCCCAGGAAGTTCTGCTGTCTCCTAGCTCTTCAGA
  AAACGAGATTTCTGATGATGATTCATACGTCAGTGACATAAGTGATAATCTGTCCTTAGACAACCTCAGT
  AATGATTTAGACAATGAAAGACAGACCTTGGGGCCTGTTCTCGAGAGTAGTGGGGAAGCCAGATCCAAAA
  GGAGGACCAGCTTGCCGGCACCGGGTCCCAACACCAGTAGCGTTAGCTTGTGGAGCATCCTGCGCAACAA
  CATTGGGAAGGACCTGTCCAAGGTGGCCATGCCGGTGGAGCTGAACGAGCCCCTGAACACGCTGCAGCGG
  CTCTGCGAGGAGCTGGAATACAGCGAGCTTCTGGACAAGGCCTCGAGAATCCCCAGTCCCCTGGAGAGGA
  TGGTGTACGTAGCAGCCTTCGCCATCTCCGCATATGCATCCAGCTACTTCCGAGCAGGCAGCAAGCCGTT
  TAACCCGGTCCTTGGAGAAACGTACGAATGCATCCGACAGGACAAGGGTTTCCAGTTTTTTGCCGAGCAG
  GTCAGCCACCACCCACCTATCTCCGCCTGCCACGCTGAGTCCGGAAACTTTGTTTTCTGGCAAGATGTGA
  GATGGAAAAATAAATTCTGGGGTAAATCCATGGAAATTGTCCCGATTGGCACAACCCACGTGACTCTGCC
  TGCTTTTGGAGATCACTTTGAGTGGAACAAAGTAACCTCCTGCATCCACAACATCTTGAGCGGACAGCGA
  TGGATCGAGCACTATGGCGAAATTGACATCAAGAACCTGAATGACGACTCCTGCCACTGCAAAGTGAACT
  TCATAAAGGCTAAGTACTGGAGCACTAATGCCCACGAGATCGAAGGCACGGTGTTCGACCGCAGTGGGAA
  GGCTGTGCACCGGCTGTTTGGGAAATGGCACGAGAGCATCTACTGTGGCGGCGCCTCCTCTTCCACATGC
  GTCTGGAGAGCAAATCCCATGCCGAAAGGCTATGAGCAGTATTATGGCTTCACACAGTTTGCACTAGAGT
  TAAATGAAATGGATCCTTTGTCAAGGTCTTTATTACCACCCACCGACACTCGGTTTAGACCAGATCAAAG
  GCTTCTAGAGGAAGGGAACATAGAGGAGGCTGAGGTTCAGAAGCAGAGGATCGAGAAGCTGCAGAGAGAG
  CGGCGGAGGGTCTTGGAGGAGAACGGTGTGGAGCACCAGCCCCGGTTTTTCAGGAAATCCAGTGACGATG
  CTTGGGTAAGCAATGGGACCTACCTGGAACTGAGGAAAGACCTTGGCTTTTCCAAGTTGGACCATCCCGT
  CTTATGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC086485
• Insert Size: 2460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC086485, AAH86485
• RefSeq Size: 3233 bp
• RefSeq ORF: 2459 bp
• Locus ID: 71720
• Cytogenetics: 6 B2.3
• Gene Summary: Phosphoinositide-binding protein which associates with both cell and endoplasmic reticulum (ER) membranes. Can bind to the ER membrane protein VAPA and recruit VAPA to plasma membrane sites, thus linking these intracellular compartments. The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface. With VAPA, may regulate ER morphology. Has a role in regulation of the actin cytoskeleton, cell polarity and cell adhesion. Binds to phosphoinositides with preference for PI(3,4)P2 and PI(3,4,5)P3. Also binds 25-hydroxycholesterol and cholesterol.[UniProtKB/Swiss-Prot Function]