Arhgef7 (NM_001113518) Mouse Untagged Clone

CAT#: MC220793

Arhgef7 (untagged) - Mouse Rho guanine nucleotide exchange factor (GEF7) (Arhgef7), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arhgef7
• Synonyms: betaPix; betaPix-b; betaPix-c; Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220793 representing NM_001113518
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGATAACACCAACAGCCAACTGGTAGTACGAGCCAAGTTTAACTTCCAGCAGACCAATGAAGATG
  AACTCTCCTTCTCAAAGGGTGATGTCATCCATGTCACACGAGTGGAGGAAGGAGGCTGGTGGGAGGGCAC
  ACACAATGGCAGGACCGGCTGGTTCCCCAGCAACTACGTTCGAGAGATCAAGCCAAGTGAGAAGCCCGTG
  TCACCCAAATCAGGGACCTTGAAGAGCCCTCCCAAAGGGTTCGATACGACTGCCATCAACAAGAGCTATT
  ACAACGTGGTGCTACAGAACATCCTGGAAACAGAGCATGAGTATTCCAAGGAGCTGCAGTCTGTGCTGTC
  CACCTACCTGCGGCCACTGCAGACCAGTGACAAGTTGAGTTCAGCAAACACTTCATATTTAATGGGAAAT
  CTAGAGGAAATATCTTCCTTCCAGCAAGTGCTTGTACAGTCCCTAGAAGAATGCACGAAGTCTCCTGAAG
  CCCAACAGAGAGTTGGTGGCTGCTTCCTGAGCCTGATGCCGCAGATGAGGACCCTGTACCTCGCTTACTG
  TGCCAACCACCCATCTGCTGTGAGCGTCCTCACAGAGCACAGTGAGGACCTAGGAGAGTTCATGGAAACA
  AAAGGTGCCAGCAGCCCTGGGATCCTGGTGCTGACCACCGGCCTGAGCAAGCCCTTCATGCGCCTGGACA
  AGTACCCCACACTGCTAAAGGAGCTGGAGAGACACATGGAGGATTATCATCCTGATAGACAAGATATTCA
  GAAGTCTATGACGGCCTTCAAAAACCTTTCAGCTCAGTGTCAAGAAGTTCGCAAGAGGAAGGAGCTGGAG
  CTGCAGATCCTGACGGAGCCCATCAGGAGCTGGGAGGGGGATGACATAAAGACCCTGGGCAGTGTCACAT
  ACATGTCCCAAGTCACCATTCAGTGTGCGGGAAGCGAGGAGAAGAATGAGAGATACCTCCTGCTCTTCCC
  AAACCTTCTGCTCATGTTGTCTGCAAGTCCCAGGATGAGTGGTTTCATCTATCAGGGGAAGCTGCCAACA
  ACAGGAATGACAATCACAAAGCTTGAGGACAGTGAAAACCATAGGAATGCATTTGAGATATCAGGGAGCA
  TGATCGAGCGGATTCTGGTGTCCTGCACCAGCCAGCAGGACTTACACGAGTGGGTGGAACACCTGCAGAA
  GCAGACGAAGGTCACATCTGTGAGCAACCCCACCATCAAACCCCACTCGGTGCCATCACACACACTTCCT
  TCCCATCCTCTCACTCCATCCAGCAAACACGCGGACAGCAAGCCCGTGGCACTGACGCCTGCGTACCACA
  CACTCCCCCACCCCTCTCACCATGGCACCCCACACACCACCATCAGCTGGGGACCCCTGGAGCCTCCGAA
  GACCCCCAAGCCTTGGAGCCTGAGTTGCCTGCGGCCTGCACCTCCCCTCCGGCCCTCAGCTGCTCTCTGC
  TACAAGGAGGATCTCAGTAAGAGCCCCAAGACCATGAAAAAGCTGCTGCCGAAGCGCAAGCCCGAGCGGA
  AGCCTTCGGACGAGGAGTTCGCTGTGCGCAAGAGCACAGCGGCGCTGGAAGAAGACGCTCAGATCCTGAA
  GGTTATCGAAGCTTATTGCACAAGTGCAAAGACGCGCCAGACCCTGAACTCAACATGGCAAGGCACTGAC
  CTGATGCATAATCACGTCTTGGCTGATGACGACCAATCAAGTCTAGACTCCTTGGGTCGTCGCAGTAGCC
  TTTCTCGTTTGGAGCCCTCAGACCTCTCGGAAGACTCTGAGTATGACAGTATATGGACAGCCCATAGTTA
  CAGAATGGGTTCTGCATCCCGTTCACGCAAAGAATCTGCTCCACAAGTGTTGCTTCCAGAAGAAGAAAAA
  ATTATAGTCGAAGAAACCAAGAGCAATGGGCAGACAGTGATAGAGGAAAAGAGCCTCGTGGATACGGTGT
  ATGCGTTAAAGGATGAAGTCCAGGAGTTAAGACAGGATAACAAGAAGATGAAGAAGTCCTTAGAGGAGGA
  ACAGAGGGCCCGCAAGGACTTAGAGAAGCTGGTGAGGAAGGTTCTAAAGAATATGAACGACCCTGCCTGG
  GATGAGACCAATCTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001113518
• Insert Size: 2118 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113518.1, NP_001106990.1
• RefSeq Size: 4665 bp
• RefSeq ORF: 2118 bp
• Locus ID: 54126
• UniProt ID: Q9ES28
• Cytogenetics: 8 A1.1
• Gene Summary: Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. May function as a positive regulator of apoptosis. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and contains multiple coding differences including translation initiation at a downstream start codon, compared to variant 1. The resulting isoform (b) is shorter than isoform a.