Polk (BC052820) Mouse Untagged Clone

CAT#: MC220775

Polk (untagged) - Mouse polymerase (DNA directed), kappa (cDNA clone MGC:60610 IMAGE:30057338), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Polk
• Synonyms: Dinb1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052820
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAACACAAAGGAAAAGGACAACTTCAAAGACGACCTCCTGCTCCGCATGGGACTAAACGATAACA
  AAGCAGGCATGGAAGGGTTGGATAAGGAGAAAATTAACAAAATTATCATGGAAGCCACAAAGGGGTCCAG
  ATTTTATGAATATGATCCCAATTTTATGGCCATGAGTCTGGACGAAGCCTACTTGAATATAACACAGCAC
  TTGCAGGAAAGGCAAGATTGGCCTGAGGACAAAAGAAGATACTTCATCAAAATGGGAAACTACTTAAAAA
  TCGACACACCCAGACAGGAAGCTAACGAGCTGACTGAGTATGAGCGGTCCATCTCCCCGCTGCTTTTTGA
  AGATAGTCCTCCTGATTTGCAACCCCAAGGAAGTCCTTTCCAACTGAACTCTGAAGAACAAAACAATCCT
  CAAATAGCCCAAAATTCAGTTGTTTTTGGAACATCAGCTGAGGAAGTGGTAAAGGAAATTCGCTTCAGAA
  TTGAACAAAAAACAACGCTGACAGCCAGCGCAGGCATCGCCCCCAATACAATGTTAGCAAAAGTGTGCAG
  TGATAAGAATAAGCCAAACGGACAGTACCAGATCCTTCCCAGCAGGAGCGCGGTGATGGACTTCATCAAG
  GACCTGCCTATTAGAAAGGTTTCTGGGATAGGAAAAGTTACAGAGAAAATGTTAATGGCTCTCGGGATTG
  TTACTTGCACAGAACTCTACCAACAGAGAGCGTTGCTGTCTCTCCTTTTCTCTGAAACCTCTTGGCATTA
  TTTTCTTCACATCGCGCTGGGTCTAGGTTCAACAGACCTGGCAAGGGATGGAGAAAGGAAAAGCATGAGT
  GTTGAAAGGACATTCAGTGAGATAAGTAAGACAGAGGAACAGTACAGCCTGTGCCAAGAACTGTGCGCTG
  AGCTCGCCCACGACCTCCAGAAGGAAGGACTTAAGGGAAGAACCGTCACCATTAAGCTGAAGAACGTGAA
  TTTTGAAGTAAAAACTCGTGCATCTACCGTTCCGGCCGCCATTTCTACTGCAGAGGAAATATTTGCCATT
  GCCAAGGAGCTGCTAAGGACAGAAGTTAATGTGGGTTCTCCACACCCCCTGCGGTTAAGACTGATGGGTG
  TCCGAATGTCTACTTTTTCCAGTGAAGATGACAGGAAACACCAACAAAGGAGCATCATTGGTTTCTTACA
  AGCTGGAAACCAAGCTTTGTCATCTACTGGGGGTAGTCTAGACAAAACTGCCAAAACTGAGCTTGCAAAG
  CCCTTAGAAATGTCTCATAAGAAGAGTTTCTTTGATAAAAAGCGATCAGAAAGAATCTCCAACTGTCAAG
  ACACATCCAGATGTAAAACTGCGGGTCAGCAAGCTTTACAGATCTTGGAACCATCCCAAGCATTAAAGAA
  GCTGAGCCAGAGTTTTGAAACATCAGAGAATTCAAATGACTGTCAGACATTTATATGTCCAGTTTGCTTT
  AGGGAGCAAGAAGGTGTCAGTCTGGAAGCCTTTAATGAACATGTAGATGAGTGTCTTGATGGACCGTCAA
  CCAGTGAGAACTCAAAAATATCCTGTTACTCACATGCTTCCTCTGCAGACATTGGTCAGAAGGAAGATGT
  ACACCCCTCTATTCCACTGTGTGAGAAACGGGGGCATGAAAATGGAGAGATCACTTCAGTAGATGGTGTA
  GATCTAACAGGGACGGAAGACAGATCATTGAAAGCAGCAAGTATGGACACTCTAGAGAATAATCGCAGCA
  AAGAGGAATGTCCTGATATTCCAGACAAGTCTTGTCCTATATCACTGGCAAATGAAACCATCAGTACATT
  AAGTAGGCAAGAATCTGTCCAGCCTTGTACAGATGAGGTAGTAACAGGACGAGCTCTAGTGTGTCCTGTT
  TGTAACCTAGAACAAGAGACTTCTGATCTTACCCTCTTCAACATACATGTGGATATTTGCTTAAATAAAG
  GTATTATCCAAGAACTGAGAAATAGTGAAGGTAATTCAGTTAAACAACCCAAAGAAAGCTCGAGAAGTAC
  TGACAGACTTCAGAAGGCTTCAGGAAGGACCAAAAGGCCACGTGGAGCAACCTCACCATCAGCTCCTCCA
  TGCTTAGCCACCATGGTACCATTGATGCTTCCGTACCTACCTGTGTGCATCTCTGTGTCCTGTGAGCAGA
  TGCAGTTAGAATTGGCCATGATGTCCATGAAGAACATTAGCTTAACATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC052820
• Insert Size: 2220 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052820, AAH52820
• RefSeq Size: 3517 bp
• RefSeq ORF: 2219 bp
• Locus ID: 27015
• Cytogenetics: 13 D1
• Gene Summary: DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls (PubMed:12432099). Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity (By similarity).[UniProtKB/Swiss-Prot Function]