Crsp2 (BC065072) Mouse Untagged Clone

CAT#: MC220774

Crsp2 (untagged) - Mouse cofactor required for Sp1 transcriptional activation, subunit 2 (cDNA clone MGC:76389 IMAGE:6830820), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Crsp2
• Synonyms: ORF1, Trap170
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC065072
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCAGTGCAGCTGGACAACCACCAGCTGATCCCTCCTGGCGGCGGCGGTGGCAGCAGCGGCGGCG
  GAGGCAGCAGCAGCGGCTCCGCGTCTGCCCCGGCTCCCCCGCCGCCCGCAGCCGCTGTGGCGGCGGCAGC
  TGCGGCGGCAGCTAGCCCTGGATACCGGCTTAGCACGCTCATCGAATTTCTGCTCCACCGGGCCTACTCG
  GAGCTTATGGTGTTGACGGACTTATTGCCAAGGAAATCTGATGTGGAAAGGAAAATAGAAATTGTACAGT
  TTGCTAGCCGGACACGTCAACTCTTCGTTCGATTATTAGCTTTAGTAAAATGGGCCAATGACGCTGGCAA
  AGTAGAAAAGTGTGCGATGATCTCAAGCTTTTTAGATCAGCAAGCTATCTTATTTGTGGATACCGCTGAC
  CGCTTGGCCTCCTTAGCTAGAGATGCCCTGGTCCATGCACGCCTGCCTAGTTTTGCTATTCCATATGCCA
  TTGATGTACTGACTACTGGCTCTTATCCACGGCTTCCAACCTGCATCAGGGACAAAATTATTCCTCCAGA
  CCCAATTACCAAAATTGAGAAACAAGCCACACTTCATCAGCTTAATCAGATTCTTAGACATAGGCTTGTA
  ACGACAGATCTTCCTCCACAGCTAGCAAATCTTACAGTGGCAAATGGCCGTGTGAAGTTTCGAGTTGAAG
  GAGAATTTGAAGCAACCTTGACAGTGATGGGTGATGACCCAGAAGTCCCGTGGCGTCTTCTCAAGCTAGA
  AATTCTAGTTGAGGATAAGGAAACAGGAGATGGGCGAGCTTTGGTTCATAGCATGCAAATCGATTTTATC
  CATCAGCTGGTCCAGTCTAGGCTCTTTGCTGATGAGAAACCTCTTCAGGACATGTACAACTGCCTACATT
  GTTTCTGCTTATCGCTTCAACTAGAAGTATTACATTCCCAGACACTAATGTTAATCCGAGAGAGGTGGGG
  AGACCTTGTACAGGTGGAAAGATACCATGCTGGAAAGAGCCTCTCCCTCTCAGTTTGGAATCAACAGGTT
  CTAGGGAGAAAAACAGGCACAGCATCTGTTCACAAAGTTACAATTAAAATCGATGAGAATGATGTCTCCA
  AGCCTTTACAGATTTTTCATGATCCTCCTTTGCCAGCTTCTGATTCTAAATTAGTAGAAAGAGCCATGAA
  GATTGACCACTTATCAATAGAAAAGCTCCTGATTGACAGTGTACACGCAAGAGCCCACCAGAGGCTGCAG
  GAACTGAAGGCCATTCTTAGAAGCTTCAACGCCAATGAAAGCTCTTCCATAGAGACTGCACTTCCAGCTC
  TTATTGTACCCATCTTGGAGCCTTGTGGTAATTCGGAGTGCCTACACATTTTTGTAGATTTGCATTCTGG
  AATGTTCCAATTGATGCTTTATGGACTTGACCCAGCCACTCTGGAGGACATGGAAAAATCTTTGAATGAT
  GACATGAAGCGGATCATCCCTTGGATTCAGCAACTTAAGTTTTGGCTGGGACAACAGCGTTGCAAACAAT
  CTATAAAACATCTGCCTACAATAACCACTGAAACGTTGCAGCTTGCCAACTATTCAACACATCCCATTGG
  AAGCCTTTCTAAGAATAAGCTGTTTATTAAACTTACTCGCCTTCCACAGTACTACATTGTTGTGGAAATG
  CTTGAGGTTCCTAATAAGCCTACGCAGCTATCATATAACTATTACTTTATGTCGGTGAGTACTGCAGATC
  GTGAGGACAGCCCTGTCATGGCACTGCTGCTGCAGCAATTCAAGGACAACATCCAGGACCTGATGTCCTA
  TACAAAGACTGGGAAACAGACCAGAACTGGTACCAAGCACAAGTTGTCTGATGACCCATGTCCGATAGAC
  TCCAAGAAAGCCAAACGATCAGGAGAAATGTGTGCCTTCAATAAAGTTCTAGCTCACTTTGTTGCTATGT
  GTGACACCAACATGCCATTTGTTGGTCTTCGATTGGAGTTGTCTAACCTCGAGATACCACATCAGGGAGT
  GCAAGTGGAAGGTGATGGCTTCAACCATGCAATTCGCTTATTAAAGATTCCTCCCTGTAAAGGAATAAGT
  GAGGAAACACAGAAGGCTCTGGACCGCTCTCTTCTTGATTGCACTTTCCGATTACAAGGTAGAAATAACC
  GCACTTGGGTGGCTGAATTAGTGTTTGCAAATTGTCCGCTTAATGGCACTTCCACCAGGGAGCAAGGTAA
  GATCTTTCACACTGTGTTTTTGCTTATGTCCATATTTTTAAAAGTATTGGTTACTATTAGTTTTTTCCTT
  TGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC065072
• Insert Size: 2313 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC065072, AAH65072
• RefSeq Size: 5251 bp
• RefSeq ORF: 2312 bp
• Locus ID: 26896
• Cytogenetics: X A1.1
• Gene Summary: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).[UniProtKB/Swiss-Prot Function]