Itih1 (BC028814) Mouse Untagged Clone

CAT#: MC220729

Itih1 (untagged) - Mouse inter-alpha trypsin inhibitor, heavy chain 1 (cDNA clone MGC:36127 IMAGE:5099284), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Itih1
• Synonyms: Intin1; ITI-HC1; Itih-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC028814
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGTGCCACAGGGCTCCGGGTGCTGCTGTGCTTGCTGCCGCTCCTCACTCTGCAGGCCAGGCCTG
  CTTTGGGCTTGGCAACAGGCAGACCCAGGGGCAGTGAGAAGCGACACGCTGTGGACACTTATTATCTTCA
  GTCAGTCAATGGTGTGTCCATCAAGAGCTTGAAAGTCAATTGCAAAGTCACCTCTCGCTTTGCCCACTAC
  GTCATCACCAGCCAAGTGGTCAACAATGCTGATAAAGCCAGGGAAGTGGCTTTTGATGTGGAAATCCCCA
  AGACAGCCTTCATCAGTGACTTTGCCATAACATCAGATGGGAAGGCGTTCATTGGGGACATAAAGGACAA
  AGTGACTGCATGGAAGCAGTACCGGAAGGCAGCCGTTTTAGGGGAGAGCGCTGGCCTTGTCAGAGCCTCG
  GGCAGAAATATGGAGCAGTTCACCATCCATATCACTGTTGGAGCCCAGAGCAAGGCCACGTTCCGGCTCA
  CCTATGAGGAGGTACTAAAGAGGAGACTGATGCAGTATGACATCACCATCAAAGTCAGACCCAAGCAGCT
  GGTACAGCATTTTGAGATCGATGTGGACATCTTTGAACCCCAGGGGATCAGCAAGCTGGACGCTCAGGCC
  TCCTTCCTCAGTGAGGAACTGGCTGCTCAAACCATCAAGAAGTCTTTCTCAGGGAAAAAGGGTCATGTGC
  TCTTCCGCCCCACTGTGAGCCAGCAGCAGTCCTGCCCCACATGTTCTACATCCTTGCTAAATGGAGAATT
  CAAGGTGACCTACGATGTCAATCGAGACAAGCTCTGTGACCTCCTGGTGGCCAACAACTACTTTACACAT
  TTCTTTGCCCCCAAAAACCTGACCAACATGAGCAAGAACCTGGTTTTTGTGATTGACATCAGTGGCTCCA
  TGGAAGGCCAGAAAGTGAGGCAGACAAAGGAGGCACTCCTTAAGATCCTGGAGGACATGAGGCCAGTAGA
  CAACTTTGACCTGGTCCTCTTTGGCTCTAAAGTGCAATCATGGAAGGGCTCCCTGGTACCTGTGTCTAAT
  GCCAATCTGCAAGCAGCTCAAGACTTTGTGAGGCGCTTTTCTTTGGCTGGAGCCACAAACCTGAATGGAG
  GCTTGCTCCGAGGAATCGAGATCTTAAACAAAGCTCAAGGAAGCCACCCAGAACTCAGCAGCCCGGCCTC
  AATTCTTATCATGTTGACAGACGGAGAGCCCACTGAAGGGGAGACGGACCGTTCCCAGATCCTCAAGAAT
  GTACGCAACGCTATCCGGGGCAGGTTCCCGCTCTACAACCTCGGCTTTGGCCACGACCTGGACTTCAGCT
  TCCTGGAGGTCATGTCCACTGAGAACAACGGATGGGCCCAGAGAATTTATGAGGACCATGATGCCACCCA
  ACAGCTACAGGGTTTCTACAATCAAGTAGCCAATCCCCTGCTGACAGATGTGGAACTGCAGTACCCCCAG
  GATGCTGTCTTGGCTCTAACCCAGCACCGACATAAACAGTACTATGACGGCTCGGAGATTGTGGTGGCTG
  GGCGCATTGCTAACCACAAACTGAACACCTTTAAGGCTGACGTTCGGGCTCGTGGGGAAAAGCAAGAATT
  CAGGGCAACCTGCCTAGTGGACGAGGAAGAGATGAAGAAACTGCTCCGAGAGCGTGGCCACGTGCTAGAG
  AATCATGTGGAGAGGCTCTGGGCCTACCTCACGATCCAGGAGCTCCTGGCTAAGCGGATGAAGACGGAAG
  GGGAGGAGAGGGCCAACCTGTCATCCCAGGTCCTGAAGATGTCACTGGACTATCATTTCGTAACGCCACT
  GACCTCTCTGACTATCAGAGGCCTGACAGACGAGGATGGGCTGGAGCCCACCATTGACAAGCCCCTGGAG
  GATTCTCAGCCCTTAGAGATGGTGGGACCCAGAAGAACGTTTGTGCTGTCAGCCATACAGCCATCTCCTA
  CAGCCCACCCCATAGACTCAAAGTTGCCACTCCGTGTGACAGGTGTGGACACCGATCCCCACTTCATCAT
  CTATGTGCCCTCAAAAGAGGATAGCTTGTGCTTCAACATCAATGAGGAACCTGGTGTGATCCTGAACCTA
  GTACAGGACCCTGACACAGGCTTCACCGTGAATGGGCAGCTCATTGGGAACAAGGCCAGCAGCCCTGGGC
  AACACGAGAGCACATACTTCGGGAGATTGGGAATCTCAAGTCCCACATCAGACTTTCAGCTGGAAGTGAC
  TCCTCAGAACATTACACTGAACCCCAGCTCCAGCGGGTCCATGTTCTCCTGGAGGGACCAGGCTGTGCTG
  CAGAAGGATGGGGTAGTGGTGACAATCAACAAGAAGAGGAACCTGGTGGTGTCTGTGGATGATGGGGCTA
  CCTTTGAGATTGTCCTGCACAGAACATGGAAGGGGAGTGCAGTCCACCAGGACTTCCTGGGTTTCTATGT
  GCTGGACAGCTTCCGGATGTCAGCCCGGACAAAGGGGCTACTGGGACAATTCTTCTCCCCACTGGATTTT
  GAAGTATTTGACCTCCACCCAGGCTCTGACCCTACAAAGACAGATGCCACAATGGTGGTGAAGAATCGGC
  AGCTGACAGTCACCCGAGGGTTGCAAAAAGACTACAGCAAGGACCCCAGACATGGAGCAGAAGTGCCTTG
  CTGGTTTGTCCACGACAATGGAGCTGGACTGATTGATGGTGTTCACACTGACTATGTCGTCTCTGACATC
  TTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC028814
• Insert Size: 2736 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC028814, AAH28814
• RefSeq Size: 2921 bp
• RefSeq ORF: 2735 bp
• Locus ID: 16424
• Cytogenetics: 14 B
• Gene Summary: This gene encodes a heavy chain of inter-alpha trypsin inhibitor (IaI) family of plasma serine protease inhibitors. IaI proteins are protein-glycosaminoglycan-protein complexes comprised of two heavy chains and a light chain. The encoded protein covalently associates with the light chain via a chondroitin sulfate moiety. Intravenous administration of the encoded protein improved survival of mice after infection with Escherichia coli. This gene is located adjacent to two other IaI heavy chain genes. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature protein. [provided by RefSeq, Oct 2015]