Hk1 (NM_001146100) Mouse Untagged Clone

CAT#: MC220727

Hk1 (untagged) - Mouse hexokinase 1 (Hk1), nuclear gene encoding mitochondrial protein, transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hk1
• Synonyms: BB404130; dea; Hk-1; Hk1-s; mHk1-s
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220727 representing NM_001146100
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCGCCGCGCAACTACTGGCATATTACTTCACCGAGCTGAAGGATGACCAAGTCAAAAAGATTGATA
  AGTATCTGTATGCCATGCGGCTCTCTGATGAAATTCTGATAGATATCCTGACACGCTTCAAGAAAGAGAT
  GAAGAATGGCCTCTCCCGGGATTATAACCCAACGGCCTCCGTCAAGATGCTGCCAACCTTTGTCCGGTCC
  ATTCCGGACGGCTCAGAAAAGGGGGATTTCATTGCACTGGATCTCGGCGGGTCTTCCTTTCGAATCCTGC
  GGGTGCAGGTGAACCACGAGAAGAGTCAGAACGTCAGCATGGAGTCTGAGGTCTACGACACCCCAGAGAA
  CATCGTGCACGGCAGTGGAAGCCAGCTTTTTGATCACGTCGCTGAATGCCTCGGAGACTTCATGGAGAAA
  AGGAAGATCAAGGACAAGAAATTACCCGTGGGATTCACGTTTTCCTTCCCGTGCCGACAATCCAAAATAG
  ACGAGGCCGTACTGATCACGTGGACAAAGCGGTTCAAAGCCAGTGGCGTGGAAGGGGCGGATGTGGTCAA
  GCTGCTGAATAAAGCCATTAAGAAGCGAGGGGACTATGACGCTAACATTGTAGCTGTGGTGAATGACACA
  GTGGGGACCATGATGACCTGCGGCTACGATGACCAACAGTGTGAAGTCGGCCTGATCATTGGCACTGGCA
  CCAATGCTTGCTACATGGAGGAACTGCGACACATCGACCTGGTGGAAGGCGATGAGGGGAGGATGTGTAT
  TAACACGGAATGGGGAGCCTTTGGGGATGATGGGTCCCTGGAAGACATTCGAACAGAGTTTGACAGAGAG
  TTAGACCGGGGATCCCTCAACCCTGGGAAACAGCTGTTCGAGAAGATGGTGAGCGGCATGTACATGGGGG
  AGCTGGTCCGGCTGATCCTGGTGAAGATGGCCAAGGAAAGCCTCTTATTTGAAGGGCGCATTACTCCAGA
  GCTGCTCACGAGGGGCAAGTTCACCACTAGCGACGTAGCCGCCATTGAAACGGATAAGGAAGGCGTTCAA
  AATGCCAAGGAAATCTTGACCCGCCTGGGAGTGGAGCCGTCTCACGATGACTGCGTATCGGTCCAGCACG
  TATGCACGATCGTCTCCTTCCGATCAGCCAACCTGGTGGCTGCCACGCTCGGTGCCATCTTGAACCGCCT
  GCGGGACAATAAGGGCACGCCCAGGCTGCGGACCACAGTTGGCGTAGACGGTTCTCTCTACAAGATGCAC
  CCACAGTATTCCCGGCGGTTCCACAAGACCCTGAGGCGCCTGGTGCCTGACTCGGACGTCCGGTTCCTCC
  TCTCGGAGAGTGGCAGTGGCAAGGGAGCCGCCATGGTGACCGCTGTGGCCTACCGCCTGGCCGAGCAGCA
  CCGGCAGATTGAGGAAACCCTGTCCCACTTCCGCCTCAGCAAGCAGGCACTGATGGAGGTGAAGAAGAAG
  CTGCGGTCAGAGATGGAAATGGGGCTGAGAAAGGAGACCAACAGCAGAGCTACGGTCAAAATGCTGCCTT
  CTTATGTTCGGAGCATCCCAGATGGGACTGAGCATGGTGACTTCCTGGCCTTGGATCTCGGAGGAACGAA
  TTTCCGAGTCCTACTGGTAAAGATCCGTAGTGGGAAAAAGAGAACAGTGGAGATGCACAACAAGATCTAC
  TCCATTCCCCTGGAAATCATGCAGGGCACCGGGGATGAGCTGTTTGATCACATTGTCTCCTGCATCTCCG
  ACTTCCTGGACTACATGGGGATCAAAGGCCCCCGGATGCCTCTGGGCTTCACCTTCTCGTTTCCCTGCAA
  GCAGACGAGCCTAGATTGCGGAATCTTGATCACGTGGACAAAGGGATTCAAAGCCACCGACTGTGTGGGT
  CACGATGTAGCCACTTTACTGAGGGATGCTGTAAAAAGGAGAGAGGAATTTGACCTGGATGTGGTGGCTG
  TGGTCAACGACACCGTGGGCACCATGATGACTTGTGCTTATGAAGAACCTTCTTGTGAGATTGGACTCAT
  CGTGGGGACTGGCAGCAATGCCTGCTACATGGAGGAGATGAAAAACGTGGAGATGGTGGAGGGGAACCAG
  GGCCAGATGTGCATCAATATGGAATGGGGTGCCTTTGGTGACAACGGCTGTCTGGACGACATCAGAACAG
  ACTTCGACAAAGTGGTGGACGAATATTCTCTAAACAGTGGGAAACAAAGGTTTGAGAAGATGATCAGTGG
  AATGTACCTGGGTGAGATCGTCCGTAACATCCTGATTGACTTCACCAAGAAAGGCTTCCTCTTCCGGGGA
  CAGATCTCTGAGCCACTCAAGACCCGAGGCATCTTCGAGACCAAGTTTCTCTCTCAGATCGAGAGTGACC
  GATTAGCGCTGCTCCAGGTGCGGGCCATCCTTCAGCAGCTGGGTCTAAACAGCACGTGCGACGACAGTAT
  CCTGGTCAAGACCGTGTGTGGGGTGGTGTCCAAGCGGGCGGCCCAGCTGTGTGGTGCCGGCATGGCCGCC
  GTGGTGGAAAAGATCCGAGAGAACAGAGGCCTAGACCACCTGAATGTAACCGTGGGCGTGGACGGGACGC
  TCTACAAACTCCATCCACACTTCTCCAGAATCATGCACCAAACAGTGAAGGAACTGTCACCAAAGTGTAC
  CGTGTCCTTCCTCCTGTCTGAAGACGGCAGCGGCAAGGGGGCCGCCCTTATCACAGCTGTGGGCGTGCGG
  CTCAGAGGAGACCCTACGAACGCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001146100
• Insert Size: 2757 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146100.1, NP_001139572.1
• RefSeq Size: 4157 bp
• RefSeq ORF: 2757 bp
• Locus ID: 15275
• Cytogenetics: 10 32.37 cM
• Gene Summary: Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine (By similarity). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed:27374331).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the ubiquitously expressed transcript and encodes isoform HK1. Its 5' end includes an exon that is unique to this transcript and encodes a distinct N-terminus containing the porin binding domain (PBD). The porin binding domain mediates association with the mitochondrial membrane.