Fert2 (BC058100) Mouse Untagged Clone

CAT#: MC220721

Fert2 (untagged) - Mouse fer (fms/fps related) protein kinase, testis specific 2 (cDNA clone MGC:67175 IMAGE:6813507), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fert2
• Synonyms: AV082135; C330004K01Rik; Fert; Fert2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC058100
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGATTTGGGAGTGACCTGAAGAACTCACAGGAAGCTGTGTTAAAGTTGCAAGACTGGGAACTACGGT
  TGCTGGAGACAGTGAAGAAATTTATGGCTCTGAGAATAAAGAGTGATAAAGAATATGCATATACTCTACA
  GAATCTTTGTAATCAAGTTGATAAAGAAAGCACTGTGCAAGTGAATTATGTCAGCAACGTGTCCAAGGTC
  ACAAAGACAGAATTGGAGAAATTAAAATCCAGCTATCGACAATTAATAAAAGAAATGAATTCTGCCAAAG
  AGAAATATAAAGAAGCCTTAGCGAAAGGGAAGGAAACAGAAAAGGCCAAGGAGCGCTATGACAAAGCTAC
  AATGAAGCTTCATATGCTGCATAATCAGTATGTGTTGGCACTGAAGGGGGCACAGCTCCATCAGAGCCAG
  TACTATGATACCACACTTCCTCTGCTGCTGGACTCCGTGCAAAAGATGCAAGAAGAAATGATAAAAGCAC
  TAAAAGGTATATTTGATGACTATAGCCAGATAACCAGCCTTGTTACAGAGGAAATAGTGAATGTCCATAA
  AGAGATTCAAATGTCTGTTGAACAGATAGACCCTAGCACCGAATATAATAATTTCATAGATGTTCACAGA
  ACAACAGCTGCTAAAGAACAAGAAATCGAATTTGATACTTCCCTATTAGAAGAAAATGAAAATCTTCAGG
  CAAATGAGATCATGTGGAATAATTTAACAGCAGACAGTTTGCAAGTCATGTTGAAGACTTTAGCGGAAGA
  GCTCACACAGACCCAGCAGATGCTTTTACACAAGGAGGCGGCCGTCCTGGAGCTAGAGAAGAGGATTGAA
  GAATCTTTTGAGACCTGTGAAAAGAAGTCTGACATTGTGCTTCTTCTGGGCCAAAAACAGGCACTTGAAG
  AGTTGAAACAGTCAGTCCAGCAGTTGCGATGCACTGAGGCAAAGTGTGCAGCACAGAAAGCTTTGCTGGA
  GCAAAAAGTACAGGAAAATGATGGGAAAGAACCACCTCCTGTGGTGAATTACGAAGAAGACGCGCGGTCA
  GTCACATCCATGGAAAGAAAGGAGAGGCTATCCAAATTTGAGTCTATTCGTCATTCAATTGCTGGGATAA
  TTAAGTCTCCAAAGTCGGTACTCGGATCTTCAACACAGGTGTGTGATGTGATCTCTGTGGGTGAGAGGCC
  CCTGGCGGAGCATGACTGGTACCATGGTGCCATCCCCAGGATAGAGGCACAGGAACTGCTGAAGCAGCAA
  GGAGACTTCCTGGTGCGGGAGAGCCATGGGAAACCTGGTGAATATGTCCTTTCTGTATATTCTGACGGAC
  AAAGGAGGCACTTTATCATACAATTTGTCGATAATCTGTATCGATTCGAGGGCACCGGGTTTTCAAACAT
  TCCCCAGCTTATAGATCACCACTTCAATACAAAGCAAGTCATCACCAAGAAGTCTGGGGTGGTTCTGCTC
  AACCCCATCCCAAAGGATAAGAAATGGGTTCTCAATCATGAAGATGTTTCATTGGGAGAATTACTGGGCA
  AGGGGAATTTTGGTGAAGTGTATAAGGGCACACTAAAGGATAAAACTCCTGTTGCCATTAAAACGTGCAA
  GGAAGACCTTCCTCAGGAATTAAAAATAAAGTTTCTACAGGAAGCCAAAATTCTGAAGCAATATGATCAT
  CCCAATATTGTCAAACTGATAGGCGTGTGCACACAAAGACAGCCTGTCTACATCATTATGGAACTGGTCC
  CAGGGGGTGATTTTCTGACCTTCCTGAGGAAGAGGAAGGACGAGCTGAAGCTGAAGCAGTTGGTGAGATT
  TTCCTTGGACGTTGCTGCTGGCATGTTGTATCTCGAGAGCAAGAACTGCATTCACAGGGACCTGGCTGCA
  CGGAACTGCCTGGTGGGTGAAAATAATACTCTGAAAATCAGTGACTTTGGAATGTCTCGGCAAGAAGACG
  GTGGAGTGTATTCATCTTCTGGCTTAAAGCAGATTCCTATTAAATGGACAGCACCGGAAGCTCTTAATTA
  TGGGAGATACAGTTCTGAAAGTGACGTGTGGAGCTTCGGCATCCTCCTCTGGGAGACCTTCAGCCTAGGA
  GTCTGCCCGTACCCTGGAATGACAAACCAGCAAGCGCGAGAGCAAGTGGAGAGAGGATACCGGATGTCAG
  CCCCACAGAACTGTCCAGAGGAGGTTTTTACAATCATGATGAAGTGTTGGGATTACAAGCCTGAAAACCG
  CCCTAAGTTCAACGACCTTCACAAAGAGCTCACTGTCATCAAGAAGATGATCACATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC058100
• Insert Size: 2298 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC058100, AAH58100
• RefSeq Size: 2948 bp
• RefSeq ORF: 2297 bp
• Locus ID: 14158
• Cytogenetics: 17 E1.1
• Gene Summary: Tyrosine-protein kinase that acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. Acts downstream of EGFR, KIT, PDGFRA and PDGFRB. Acts downstream of EGFR to promote activation of NF-kappa-B and cell proliferation. May play a role in the regulation of the mitotic cell cycle. Plays a role in the insulin receptor signaling pathway and in activation of phosphatidylinositol 3-kinase. Acts downstream of the activated FCER1 receptor and plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Plays a role in the regulation of mast cell degranulation. Plays a role in leukocyte recruitment and diapedesis in response to bacterial lipopolysaccharide (LPS). Phosphorylates CTTN, CTNND1, PTK2/FAK1, GAB1, PECAM1 and PTPN11. May phosphorylate JUP and PTPN1. Can phosphorylate STAT3 according to PubMed:10878010 and PubMed:19159681, but clearly plays a redundant role in STAT3 phosphorylation. According to PubMed:11134346, cells where wild type FER has been replaced by a kinase-dead mutant show no reduction in STAT3 phosphorylation. Phosphorylates TMF1. Isoform 3 lacks kinase activity.[UniProtKB/Swiss-Prot Function]