Ercc2 (BC034517) Mouse Untagged Clone

CAT#: MC220718

Ercc2 (untagged) - Mouse excision repair cross-complementing rodent repair deficiency, complementation group 2 (cDNA clone MGC:27532, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ercc2
• Synonyms: AA407812; AU020867; AW240756; CXPD; Ercc-2; Mhdarco15; RCO015; XPD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC034517
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTCAACGTGGACGGGCTGCTGGTCTACTTCCCGTACGACTACATCTACCCGGAGCAGTTCTCCT
  ACATGCTGGAGCTCAAGCGCACGCTGGACGCCAAGGGTCATGGAGTCCTGGAGATGCCCTCGGGCACTGG
  GAAGACAGTGTCCCTATTGGCCCTGATTGTGGCCTATCAGCGGGTCATAGAAGAGCTTCGTAAGCTACTC
  AGCTTCTACGAGCAGCAGGAGGGCGAGAAGCTGCCGTTCTTAGGACTGGCTCTGAGCTCAAGAAAGAACC
  TGTGCATTCATCCCGAGGTGACTCCACTGCGCTTTGGGAAGGATGTTGATGGGAAGTGTCACAGCCTAAC
  GGCGTCGTATGTGCGGGCACAGTACCAGCAGGATGCCAGCCTGCCTCACTGCCGCTTCTATGAGGAATTT
  GACATCCATGGACGCCAGATGCCGCTCCCTGCGGGCATCTACAACCTGGATGACCTGAAGGCCCTAGGGC
  ATCGCCAGGGCTGGTGCCCCTACTTCCTGGCTCGATACTCGATCCTGCATGCCAACGTGGTGGTTTACAG
  CTACCACTACCTCCTGGACCCCAAGATCGCAGACCTGGTATCCAAAGAGCTGGCTCGCAAGGCTGTTGTG
  GTCTTCGATGAAGCTCACAACATCGACAATGTCTGCATTGACTCCATGAGTGTCAACCTCACCCGCAGGA
  CTCTGGACCGTTGCCAGAGCAACTTAGACACCCTACAGAAGACCGTGCTCAGGATCAAGGAGACGGATGA
  GCAGCGGCTGCGGGATGAGTACCGGCGCCTGGTGGAGGGCCTGCGGGAGGCCAGTGTGGCCCGGGAGACA
  GATGCCCACCTGGCCAACCCTGTGCTGCCGGACGAGGTGCTGCAGGAGGCTGTGCCTGGCTCCATCCGTA
  CGGCTGAGCACTTCCTGGGCTTTCTGCGGCGGCTGCTGGAGTATGTCAAGTGGCGTCTGCGCGTGCAGCA
  TGTGGTGCAGGAGAGTCCACCTGCCTTTCTGAGCGGCCTGGCCCAGCGGGTGTGCATCCAGCGCAAGCCC
  CTCAGGTTCTGTGCTGAACGCCTGCGCTCCCTGCTGCACACCCTGGAGATTGCCGACCTGGCCGACTTCT
  CCCCGCTCACACTCCTTGCTAACTTCGCCACTCTCGTCAGCACTTACGCCAAGGGCTTCACCATTATCAT
  TGAGCCCTTTGACGACAGGACCCCCACCATCGCCAACCCCGTTCTGCACTTCAGCTGTATGGACGCCTCC
  TTGGCCATCAAGCCTGTGTTTGAGCGCTTCCAGTCTGTCATCATCACTTCTGGGACACTGTCCCCACTGG
  ACATCTACCCCAAGATCCTGGACTTCCACCCTGTCACAATGGCAACCTTCACCATGACGCTGGCCCGAGT
  CTGCCTCTGCCCGATGATCATTGGCCGTGGTAATGACCAGGTAGCAATCAGCTCCAAATTTGAGACCAGA
  GAAGATATTGCTGTGATCCGAAACTATGGCAACCTCCTGCTGGAGATGTCCGCCGTGGTCCCAGATGGCA
  TTGTGGCCTTCTTTACCAGCTACCAGTACATGGAAAGCACCGTGGCCTCCTGGTATGAGCAGGGCATCCT
  TGAGAACATCCAGAGGAACAAACTGCTCTTCATTGAGACCCAGGATGGGGCTGAGACCAGTGTGGCCCTG
  GAGAAGTACCAAGAGGCATGCGAGAATGGCCGTGGGGCCATTCTGCTCTCAGTGGCTCGGGGCAAAGTAT
  CAGAAGGGATTGACTTTGTACACCACTACGGACGGGCTGTGATCATGTTTGGAGTTCCCTATGTCTATAC
  CCAGAGCCGAATTCTCAAGGCCCGGCTAGAGTATCTGCGGGACCAGTTCCAGATCCGAGAGAACGACTTC
  CTCACCTTTGATGCTATGCGCCATGCAGCCCAGTGTGTGGGTCGTGCCATCAGGGGCAAGACGGACTATG
  GACTCATGGTCTTTGCTGACAAGCGGTTTGCTCGGGCGGACAAGCGTGGTAAGCTGCCTCGCTGGATCCA
  GGAGCACCTGACCGACTCCAACCTCAACCTGACCGTGGATGAGGGTGTACAGGTCGCCAAGTACTTCCTG
  CGGCAGATGGCGCAGCCCTTCCACCGGGAGGATCAGCTGGGCCTGTCGCTGCTCAGCCTGGAGCAGCTGC
  AGTCAGAGGAGACACTACAGCGAATTGAGCAGATCGCACAGCAGCTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC034517
• Insert Size: 2220 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC034517, AAH34517
• RefSeq Size: 3500 bp
• RefSeq ORF: 2219 bp
• Locus ID: 13871
• Cytogenetics: 7 9.62 cM
• Gene Summary: ATP-dependent 5'-3' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATP-dependent helicase activity of XPD/ERCC2 is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. XPD/ERCC2 acts by forming a bridge between CAK and the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers.[UniProtKB/Swiss-Prot Function]