Smc3 (BC057345) Mouse Untagged Clone

CAT#: MC220711

Smc3 (untagged) - Mouse structural maintenace of chromosomes 3 (cDNA clone MGC:67063 IMAGE:6811961), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Smc3
• Synonyms: HCAP, SmcD, Bamacan
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220711 representing BC057345
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATAACTTTGAGTGTGAACCAGCTTTCTATACTTGTGTGGAAGTCACTGCTGGTAACAGGTTATTTT
  ATCACATTGTTGATTCAGATGAAGTCAGCACGAAAATTTTAATGGAGTTCAATAAAATGAATCTTCCTGG
  AGAGGTGACTTTCCTGCCTCTTAACAAGTTAGATGTGAGAGATACTGCCTATCCTGAAACTAACGATGCT
  ATTCCTATGATCAGTAAGCTGAGGTACAATCCCAGATTTGACAAAGCTTTCAAACATGTATTTGGAAAGA
  CACTCATCTGTCGGAGCATGGAAGTTTCAACTCAGCTGGCCCGGGCCTTCACTATGGACTGTATTACTTT
  GGAAGGTGATCAAGTCAGCCATCGAGGTGCTCTGACTGGAGGTTATTACGACACAAGAAAGTCTCGACTT
  GAGTTACAGAAAGACGTTAGGAAGGCAGAGGAGGAGCTGGGTGAGCTGGAGGCTAAGCTCAATGAAAACC
  TACGCAGGAACATTGAAAGGATTAATAATGAAATTGACCAGTTGATGAACCAAATGCAGCAGATAGAGAC
  CCAACAAAGAAAATTTAAAGCATCCAGAGATAGCATATTATCAGAGATGAAGATGCTAAAAGAGAAGAGA
  CAGCAATCAGAAAAGACCTTCATGCCAAAGCAACGTAGCTTACAAAGCTTGGAGGCAAGTCTGCATGCTA
  TGGAGTCCACCAGAGAGTCACTGAAAGCGGAGCTAGGAACGGATTTGCTTTCTCAACTCAGTCTGGAAGA
  TCAGAAAAGAGTCGACGCTCTGAATGATGAAATCCGTCAGCTGCAGCAGGAAAACAGACAGCTGCTAAAT
  GAAAGAATTAAACTAGAAGGCATTATTACTCGAGTAGAGACTTACCTGAATGAGAATCTGAGGAAACGCT
  TGGACCAAGTAGAACAGGAACTTAATGAACTGAGAGAGACAGAAGGTGGTACTGTTCTTACTGCCACAAC
  ATCAGAACTTGAAGCTATTAATAAAAGAGTAAAAGATACTATGGCAAGATCAGAAGATTTGGACAATTCC
  ATTGACAAAACAGAAGCTGGAATTAAAGAGCTCCAGAAAAGTATGGAGCGCTGGAAAAATATGGAGAAAG
  AACACATGGATGCCATAAATCATGATACTAAAGAGCTGGAGAAGATGACCAACCGGCAAGGCATGCTGTT
  GAAGAAGAAGGAGGAGTGTATGAAGAAGATCCGGGAGCTGGGGTCCCTCCCCCAGGAAGCGTTTGAGAAG
  TACCAGACACTGAGCCTGAAGCAGTTGTTTCGAAAACTGGAGCAATGCAACACAGAGTTAAAGAAGTACA
  GCCACGTGAACAAAAAGGCTCTAGATCAGTTTGTGAATTTCTCTGAGCAGAAGGAAAAGCTGATAAAGCG
  GCAAGAGGAATTGGATAGAGGCTACAAATCAATCATGGAATTGATGAATGTACTTGAACTTAGAAAATAT
  GAAGCTATTCAGTTAACTTTCAAACAGGTATCTAAGAACTTCAGTGAAGTTTTCCAGAAGTTGGTACCTG
  GTGGCAAAGCGACTTTGGTGATGAAGAAAGGAGATGTGGAGGGCAGCCAGTCTCAGGACGAAGGAGAAGG
  GAGTGGCGAGAGTGAGCGGGGCTCTGGGTCACAGAGCAGTGTTCCATCAGTTGACCAGTTCACAGGAGTT
  GGGATCAGGGTGTCATTTACAGGGAAGCAAGGGGAGATGAGAGAAATGCAGCAGCTCTCAGGAGGACAGA
  AATCTCTGGTAGCCCTTGCTCTCATCTTTGCCATTCAGAAATGCGACCCTGCTCCTTTTTACCTGTTTGA
  TGAGATCGACCAAGCTTTGGATGCTCAGCACAGAAAAGCCGTGTCAGATATGATTATGGAGCTAGCCGTG
  CATGCCCAGTTTATTACCACTACTTTCAGGCCTGAACTGCTTGAGTCAGCTGACAAATTCTATGGTGTAA
  AGTTCAGAAATAAGGTTAGTCACATTGATGTGATCACAGCAGAGATGGCCAAAGATTTTGTAGAAGACGA
  TACCACGCATGGTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC057345
• Insert Size: 2046 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057345, AAH57345
• RefSeq Size: 2711 bp
• RefSeq ORF: 2045 bp
• Locus ID: 13006
• Cytogenetics: 19 D2
• Gene Summary: Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.[UniProtKB/Swiss-Prot Function]