Vav1 (NM_001163816) Mouse Untagged Clone

CAT#: MC220694

Vav1 (untagged) - Mouse vav 1 oncogene (Vav1), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vav1
• Synonyms: Vav; vav-T
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220694 representing NM_001163816
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCTGGCGACAGTGCACCCACTGGCTGATCCAGTGTCGGGTGCTGCCTCCCAGCCACCGTGTGA
  CCTGGGAGGGGGCCCAGGTGTGTGAGCTGGCACAGGCACTGCGGGACGGTGTCCTCTTGTGCCAATTGCT
  TAACAACCTGCTTCCCCAGGCCATTAATCTTCGCGAGGTTAACTTGCGGCCCCAGATGTCCCAGTTCCTT
  TGTCTTAAGAACATTCGAACCTTCCTGTCTACTTGCTGTGAGAAGTTCGGCCTCAAGCGCAGTGAACTCT
  TTGAGGCTTTTGACCTCTTCGATGTGCAGGACTTTGGAAAGGTCATCTACACCCTGTCTGCTCTGTCATG
  GACACCCATTGCCCAGAACAAAGGAATCATGCCCTTCCCAACAGAGGACAGCGCTCTGAACGACGAAGAT
  ATTTACAGTGGCCTTTCAGACCAGATTGATGACACCGCAGAGGAAGACGAGGACCTTTATGACTGCGTGG
  AAAATGAGGAGGCAGAGGGGGACGAGATCTACGAGGACCTAATGCGCTTGGAGTCGGTGCCTACGCCACC
  CAAGATGACAGAGTATGATAAGCGCTGCTGCTGCCTGCGGGAGATCCAGCAGACGGAGGAGAAGTATACA
  GACACACTGGGCTCCATCCAGCAGCACTTCATGAAGCCTCTGCAGCGATTCCTTAAGCCTCAAGACATGG
  AGACCATCTTTGTCAACATTGAGGAGCTGTTCTCTGTGCATACCCACTTCTTAAAGGAACTGAAGGATGC
  CCTGGCTGGCCCGGGAGCAACAACACTGTATCAGGTCTTCATCAAGTACAAGGAGAGGTTCCTGGTTTAT
  GGCCGTTATTGCAGTCAGGTGGAGTCAGCCAGCAAGCACTTGGATCAAGTGGCCACAGCACGGGAGGATG
  TGCAGATGAAGCTGGAGGAATGTTCTCAAAGAGCTAACAATGGCCGATTCACCCTACGGGATCTGCTGAT
  GGTACCTATGCAGCGGGTGCTGAAGTACCACCTCCTTCTCCAGGAGCTAGTGAAACACACACAGGATGCT
  ACAGAGAAGGAGAACCTGCGGTTGGCCCTGGACGCCATGAGGGACCTGGCACAGTGCGTGAACGAGGTCA
  AGAGGGACAATGAAACCCTACGGCAGATCACAAACTTTCAGCTGTCCATTGAGAACCTGGACCAGTCTCT
  GGCTAACTATGGCCGGCCCAAGATTGACGGTGAGCTCAAGATTACCTCAGTGGAGCGTCGCTCAAAGACA
  GACAGGTATGCCTTCCTGCTGGACAAAGCACTGCTCATCTGTAAACGCCGCGGGGACTCTTACGACCTCA
  AAGCCTCGGTGAACTTGCACAGCTTCCAAGTTCGAGATGACTCCTCCGGGGAGCGAGACAACAAGAAGTG
  GAGCCATATGTTCCTTCTGATTGAGGATCAAGGCGCCCAGGGCTATGAGCTGTTCTTCAAGACTCGGGAG
  CTGAAGAAGAAGTGGATGGAACAGTTCGAAATGGCCATCTCCAACATTTACCCAGAGAATGCTACAGCCA
  ATGGGCATGATTTTCAGATGTTCTCCTTTGAGGAGACCACTTCCTGCAAGGCCTGCCAGATGTTACTCAG
  AGGCACATTCTACCAGGGATATCGCTGTTACAGGTGCCGGGCACCTGCACACAAGGAGTGTCTGGGGAGA
  GTGCCTCCCTGTGGTCGCCATGGGCAAGATTTCGCAGGAACCATGAAGAAGGACAAGCTCCATCGAAGGG
  CCCAGGACAAGAAAAGGAATGAATTGGGTCTGCCTAAGATGGAAGTGTTTCAGGAATACTATGGGATCCC
  TCCTCCACCTGGAGCCTTTGGGCCATTTTTACGGCTCAACCCTGGGGACATTGTGGAGCTCACTAAGGCA
  GAGGCTGAGCACAACTGGTGGGAGGGAAGGAATACTGCTACAAATGAAGTCGGCTGGTTTCCCTGTAACA
  GAGTGCATCCCTATGTCCACGGCCCTCCTCAGGACCTGTCTGTGCATCTCTGGTATAACGTGGAGGTCAA
  GCATATTAAAATCATGACGTCAGAGGGGTTGTACCGGATCACAGAGAAGAAGGCTTTCCGGGGCCTTCTG
  GAACTGGTAGAGTTTTATCAGCAGAATTCCCTCAAAGATTGCTTCAAGTCGTTGGACACCACCTTGCAGT
  TTCCTTATAAGGAACCTGAGAGGAGAGCCATCAGCAAGCCACCAGCTGGAAGCACCAAGTATTTTGGCAC
  TGCCAAAGCCCGCTACGACTTCTGTGCCCGGGACAGGTCGGAACTGTCCCTTAAGGAGGGTGATATCATC
  AAGATCCTCAATAAGAAGGGACAGCAAGGCTGGTGGCGTGGGGAGATCTACGGCCGGATCGGCTGGTTCC
  CTTCTAACTATGTGGAGGAAGACTATTCCGAATATTGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001163816
• Insert Size: 2421 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163816.1, NP_001157288.1
• RefSeq Size: 4051 bp
• RefSeq ORF: 2421 bp
• Locus ID: 22324
• Cytogenetics: 17 29.76 cM
• Gene Summary: Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (3) that is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.