Mapk8ip1 (BC072578) Mouse Untagged Clone

CAT#: MC220692

Mapk8ip1 (untagged) - Mouse mitogen activated protein kinase 8 interacting protein 1 (cDNA clone MGC:99893 IMAGE:6402485), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mapk8ip1
• Synonyms: JIP-1, IB1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC072578
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCTGGTACTGAAGATGGATTCGAGCCCAGACAATGACAGCTGGTTGGAGGATCAGTGGGAGCACT
  GGCTCACCCATGACATCAGCCTGGAGGAGTTTGAGGATGAAGACCTTTCGGAGATCACTGACGAGTGTGG
  CATCAGCCTGCAGTGCAAAGACACCCTGTCTCTCCGGCCCCCGCGCGCCGGGCTGCTGTCTGCGGGTAGC
  AGCGGCAGCGCGGGGAGCCGGCTGCAGGCGGAGATGCTGCAGATGGACCTGATCGACGCGGCAGGTGACA
  TTCCGGGCGCCGAGGACGACGAGGAGGAGGAGGACGACGAGCTCGCTGCCCAACGACCAGGAGTGGGGCC
  TCCCAAAGCGGAGTCCAACCAGGATCCGGCGCCTCGCAGCCAGGGCCAGGGCCCGGGCACAGGCAGCGGA
  GACACCTACCGACCCAAGAGGCCTACCACGCTCAACCTTTTCCCGCAGGTGCCGCGGTCTCAGGACACGC
  TGAATAATAACTCTTTAGGCAAAAAGCACAGTTGGCAGGACCGTGTGTCTCGATCATCCTCCCCTCTGAA
  GACAGGAGAACAGACGCCTCCACATGAACACATCTGCCTGAGTGATGAGCTGCCACCCCAGGGCAGTCCT
  GTTCCCACCCAGGACCGCGGCACTTCCACCGACAGCCCTTGTCGCCGAAGTGCAGCCACCCAGATGGCAC
  CTCCAAGCGGTCCCCCTGCCACTGCGCCTGGTGGCCGGGGCCACTCCCATCGAGACCGAATCCACTACCA
  GGCAGATGTGCGGCTCGAGGCGACTGAGGAGATCTACCTGACCCCAGTGCAGAGGCCCCCAGACCCTGCA
  GAACCCACCTCCACCTTCATGCCACCCACGGAGAGCCGGATGTCAGTTAGCTCCGATCCAGACCCTGCCG
  CTTACTCTGTAACTGCGGGGCGGCCACACCCCTCCATCAGTGAAGAGGATGAGGGCTTCGACTGCCTGTC
  ATCCCCAGAGCGAGCTGAGCCACCAGGTGGAGGGTGGCGGGGAAGCCTCGGGGAGCCACCACCGCCTCCA
  CGGGCCTCACTGAGCTCGGACACCAGCGCACTGTCCTACGACTCGGTCAAGTACACACTGGTGGTGGATG
  AACATGCCCAGCTTGAGTTGGTGAGCCTGCGGCCGTGCTTTGGAGATTACAGTGACGAAAGCGACTCTGC
  CACTGTCTATGACAACTGTGCCTCTGCCTCCTCGCCCTACGAGTCAGCCATTGGTGAGGAGTATGAGGAG
  GCCCCTCAGCCCCGGCCTCCCACCTGCCTCTCAGAGGACTCCACCCCGGATGAGCCTGATGTCCACTTCT
  CTAAGAAGTTTCTGAATGTCTTCATGAGTGGCCGCTCTCGTTCCTCCAGTGCTGAGTCCTTTGGGCTGTT
  CTCCTGCGTCATCAATGGGGAGGAGCATGAGCAAACCCATCGGGCTATATTCAGGTTTGTGCCTCGGCAT
  GAAGATGAACTTGAGCTGGAAGTGGATGACCCCCTGCTGGTGGAGCTGCAGGCAGAAGACTATTGGTATG
  AGGCCTATAACATGCGCACCGGAGCCCGCGGGGTCTTCCCTGCCTACTATGCCATTGAGGTCACCAAGGA
  GCCTGAGCACATGGCAGCCCTTGCCAAAAACAGCGACTGGATTGACCAGTTCCGGGTGAAGTTCCTGGGG
  TCTGTCCAGGTTCCTTATCACAAGGGCAATGATGTCCTCTGTGCTGCTATGCAAAAGATCGCCACCACCC
  GCCGGCTCACCGTGCACTTTAACCCGCCCTCCAGCTGTGTCCTTGAGATCAGTGTCAGGGGTGTCAAGAT
  AGGCGTCAAAGCTGATGATGCTCTGGAGGCCAAGGGAAATAAATGTAGCCACTTCTTCCAGCTAAAGAAC
  ATCTCTTTCTGTGGATACCATCCAAAGAATAACAAGTACTTTGGGTTTATCACTAAGCACCCTGCTGACC
  ACCGGTTTGCCTGCCATGTCTTTGTGTCTGAAGATTCCACCAAAGCCCTGGCGGAGTCTGTGGGGCGTGC
  ATTTCAGCAGTTCTACAAGCAGTTTGTGGAGTATACCTGTCCTACAGAAGATATCTACTTGGAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC072578
• Insert Size: 2097 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC072578, AAH72578
• RefSeq Size: 3138 bp
• RefSeq ORF: 2096 bp
• Locus ID: 19099
• Cytogenetics: 2 E1
• Gene Summary: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Required for JNK activation in response to excitotoxic stress. Cytoplasmic MAPK8IP1 causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and thus inhibiting the JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly, or indirectly, regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. Functions as an anti-apoptotic protein and whose level seems to influence the beta-cell death or survival response (By similarity). Acts as a scaffold protein that coordinates with SH3RF1 in organizing different components of the JNK pathway, including RAC1 or RAC2, MAP3K11/MLK3 or MAP3K7/TAK1, MAP2K7/MKK7, MAPK8/JNK1 and/or MAPK9/JNK2 into a functional multiprotein complex to ensure the effective activation of the JNK signaling pathway. Regulates the activation of MAPK8/JNK1 and differentiation of CD8(+) T-cells (PubMed:23963642).[UniProtKB/Swiss-Prot Function]