Rnf111 (BC069835) Mouse Untagged Clone

CAT#: MC220666

Rnf111 (untagged) - Mouse ring finger 111 (cDNA clone MGC:76599 IMAGE:30094289), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rnf111
• Synonyms: ARK, Arkadia
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC069835
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCAATGGACTCCTGAATTTAACGAGCTCTACACCCTAAAAGTGGCTATGAAGAGTGGGACTCCTG
  ATGCGCCCACTACACAGGAGAGTCTGAAGGCGGTCCTTCTGCACCCACAGCCCCTAGGGGCCACCAAGAG
  CTTCCCTGCAGAAGTGGAGATGATTAATAGCAAAGTGGGGAATGAATTCTCTCATCTGTGTGATGATTCT
  CAAAAACAAGAGAAGGACATGACTGGCAACCAGCAAGAACAAGAAAAAAGTGGTGTTGTGAGAAAAAAGC
  GCAAAAGCCAGCAGGCTGGCCCCTCATATGTGCAGAACTGTGTCAAAGAAAATCAGGAAATCCTAGGGCG
  CAGACAACAGCTAGAGACACCGAGTGATGAGGATAATGACTCTTCCTTAAGTGAGTGTCTGTCTTCTCCC
  TCATCTAGTCTCCATTTTGGGGGCTCTGATACAGTGACTTCAGATGAGGATAAAGAAGTCTCGGTGAGAC
  ATACCCAGCCTGTTCTGAGTGCTAAAAGTAGAAGTCACAGTGCAAGGTCTCACAAGTGGCCTCGAACTGA
  GGCAGATCCTGTGCCAAGTTTGTTAATGAAAAGACCTTGTTTTCATGGCAGTGCACTAAGGAGAGTCACA
  TGCAGAAAGAGGCTGGTGAAGAGCAGCTCCTCGCAGAGGACACAGAAGCAGAAGGAGAGGATGCTAGTGC
  AGAGGAAGAAGCGGGAGGCGCTAGCTCAGAGGAAGTATGCTCTTCTCTCCAGCTCCAGTAGCTCCAGTGA
  GAATGACCTCAGCAGCGATTCCTCGTCCAGCTCCTCCACCGATGGAGAAGAGGACCTGTGTGCATCTGCC
  AGTGAGAACCCCAGCAACCCCGCTGCTCCCTCAGGAAGTATTGATGAAGATGTTGTGGTGATAGAAGCCT
  CCTTCACTCCCCAGGTCACTGCCAATGAAGAAATTAATGTTACCTCAACTGACAGTGAAGTGGAGATTGT
  TACAGTTGGAGAAAGCTATCGGTCTCGTTCAACCCTTGGACACTCCAGATCTCATTGGAGCCAAGGTTCA
  AGTTCGCATACAGGACGGCCACAGGAGTCTCGAAACCGCAGTAGGATCTCTACTGTTATACAGCCCCTGA
  GGCAGAATGCAGCAGAAGTTGTGGACCTTACTGTTGATGAAGATGAACCTACTATAGTACCAACCACTTC
  TGCTAGAATGGACTCACAAACTACTAGTGCTTCCATTAACAATTCAAATCCATCTACCTCTGAGCAAGCC
  TCTGATACTACTTCAACTGTGGCCAGTAGCCAGCCTTCCACAGTGTCAGAGACTGAAGCTACTCTTACAA
  GCAATAGTGCAACTGGTTCTTCTGTGGGCGATGACGTGAGGAGAACTGCATCTAGTGCTGTCCCAGAAAG
  TGGTCCTCCTGCAATGCCAAGGCTACCTTCTTGCTGCCCCCAGCACTCACCATGTGGAGGGACATCACAG
  AGCCATCATGCATTAGCACACCCACACTCAAGTTGTTTTCAGCAGCATGGCCACCATTTTCAGCATCACC
  ACCATCACCACCATACTCCCCACCCAGCTGTCCCAGTTTCTCCTTCCTTCAGTGACCCTGCTTGCCCAGT
  GGAAAGACCTCAAGTACAGGCGCCTTGTGGAGCAAATAGCAGCTCTGGTTCCAGCTACCATGACCAGCAG
  GCATTGCCAGTCGATCTGAGCAACAGTGCTCTCAGGACTCATGGAAGTGGGGGTTTCCATGGTGCATCTG
  CTTTTGACCCCTGCTGCCCTGTCACATCTTCCCGGGCTGCAGTCTTTGGCCATCAGGCTGCTGCTGCCCC
  GACTCAGCCATTATCAATAGATGGCTATGGCTCCAGCATGGTTGCCCAGCCCCAGCCCCAGCCCCCTCCA
  CAGCCTTCTCTTTCGTCATGTCGGCACTACATGCCACCTCCGTATGCTTCCTTGACAAGACCACTGCACC
  ATCAAGCCTCAGCTTGCCATCACTCTCATGGAAATGCCCCTCCCCAGACTCAGCCTCCCCCTCAAGTGGA
  TTACGTGATTCCGCATCCTGTCCATGCTTTCCATTCTCAGATATCTTCTCATGCAGCATCTCACCCTGTG
  GCACCACCGCCGCCAACACATTTAGGCAGTACAGCGGCACCCATCCCTCAGCATCTTCCTCCTGCTCACC
  AGCCGATTTCACACCATATTCCAGCCCCAGCACCTTCAGCCCAGAGACTGCACCCTCATGAAGTGATGCA
  GAGGATGGAAGTTCAGAGGAGGAGGATGATGCAGCATCCAACGCGGGCACATGAACGGCCCCCGCCCCAT
  CCACATAGGATGCACCCAAACTATGGCCATGGGCATCATATTCATGTGCCTCAAACCATGTCTTCACATC
  CTCGCCAGGCTCCAGAGAGAACTGCCTGGGAACTGGGGATTGAAGCTGGAGTAACTGCAGCTACCTACAC
  ACCTGGTGCTCTGCACCCTCACCTGGCCCATTACCACGCCCCGCCTCGACTTCATCACCTCCAGTTAGGA
  GCACTTCCTTTAATGGTTCCTGACATGGCAGGCTATCCTCACATCCGTTATATTTCTTCAGGATTGGATG
  GAGCATCATTCAGAGGTCCTTTTAGGGGCAATTTTGAGGAATTGATTCATCTGGAAGAAAGATTAGGAAA
  TGTCAATCGTGGAGCCTCCCAGGGAACAATTGAAAGATGCACATATCCACATAAATATAAAAAGAGGAAA
  CTGCACTGCAAACAAGATGGGGAAGAAGGGACTGAGGAAGACACAGAGGAAAAATGTACTATCTGTTTGT
  CTATTTTAGAGGAAGGTGAAGATGTGAGGCGCCTTCCATGTATGCACCTTTTCCACCAAGTGTGTGTTGA
  TCAGTGGCTGATCACCAATAAGAAGTGCCCCATATGCAGAGTGGACATCGAGGCCCAGCTGCCAAGTGAA
  AGTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC069835
• Insert Size: 2946 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC069835, AAH69835
• RefSeq Size: 4725 bp
• RefSeq ORF: 2945 bp
• Locus ID: 93836
• Cytogenetics: 9 39.53 cM
• Gene Summary: E3 ubiquitin-protein ligase required for mesoderm patterning during embryonic development (PubMed:11298452). Acts as an enhancer of the transcriptional responses of the SMAD2/SMAD3 effectors, which are activated downstream of BMP (PubMed:14657019). Acts by mediating ubiquitination and degradation of SMAD inhibitors such as SMAD7, inducing their proteasomal degradation and thereby enhancing the transcriptional activity of TGF-beta and BMP (PubMed:14657019). In addition to enhance transcription of SMAD2/SMAD3 effectors, also regulates their turnover by mediating their ubiquitination and subsequent degradation, coupling their activation with degradation, thereby ensuring that only effectors 'in use' are degraded (By similarity). Activates SMAD3/SMAD4-dependent transcription by triggering signal-induced degradation of SNON isoform of SKIL (By similarity). Associates with UBE2D2 as an E2 enzyme (By similarity). Specifically binds polysumoylated chains via SUMO interaction motifs (SIMs) and mediates ubiquitination of sumoylated substrates (PubMed:23530056). Catalyzes 'Lys-63'-linked ubiquitination of sumoylated XPC in response to UV irradiation, promoting nucleotide excision repair (By similarity). Mediates ubiquitination and degradation of sumoylated PML (PubMed:23530056). The regulation of the BMP-SMAD signaling is however independent of sumoylation and is not dependent of SUMO interaction motifs (SIMs) (PubMed:23530056).[UniProtKB/Swiss-Prot Function]