Trap1 (NM_026508) Mouse Untagged Clone

CAT#: MC220656

Trap1 (untagged) - Mouse TNF receptor-associated protein 1 (Trap1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trap1
• Synonyms: 2410002K23Rik; HSP75
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220656 representing NM_026508
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTGCGAGCTGCGAGCTGTGTTGCTGTGGGGCCGCGGGCTGCAGACTGTACTGCGGGCCCCCGCGC
  TGGCTGGAGTTCGGCGAGGAAAGCCAGTTCTGCACCTTCAGAAGACTACAGTCCAGTTTAGGGGCCCCAC
  ACAAAGTCTGGCTTCAGGGATCTCTGCAGGACAGTTATACAGCACACAGGCAGCCGAGGACAAGGAGGAG
  GAGAGCCTGCACTCCATCATCAGCAACACTGAGGCAGTGCGGGGTTCTGTCTCCAAACATGAGTTCCAGG
  CAGAGACAAAGAAACTTTTGGACATCGTAGCCCGTTCTCTGTACTCAGAAAAAGAGGTGTTCATACGAGA
  GCTCATCTCCAATGCCAGTGATGCCTTGGAGAAACTGCGGCACAAGCTGGTGTGTGAAGGCCAGGTGCTG
  CCAGAAATGGAGATTCACCTTCAGACGGATGCCAAGAAGGGCACTATTACCATTCAGGACACTGGCATTG
  GGATGACACAGGAGGAGCTGGTGTCCAACCTTGGCACAATTGCCAGATCGGGGTCAAAGGCCTTCCTGGA
  AGCACTGCAGAACCAGGCAGAGACCAGCAGCAAGATCATTGGTCAGTTTGGAGTGGGTTTCTATTCAGCC
  TTCATGGTAGCTGACAAGGTTGAAGTCTATTCTCGATCAGCAGCTCCAGAGAGCCCAGGTTACCAGTGGC
  TTTCAGATGGTTCTGGAGTGTTTGAAATTGCCGAAGCTTCAGGAGTTAGACCTGGGACCAAAATAATCAT
  CCACCTCAAGTCAGACTGTAAAGATTTTGCCAGCGAGTCCCGGGTACAAGATGTGGTAACAAAGTACAGT
  AACTTTGTCAGCTTCCCCTTGTACCTTAATGGAAAGCGGATTAACACTTTGCAGGCCATCTGGATGATGG
  ACCCAAAGGACATCAGTGAATTTCAGCATGAGGAATTCTACCGTTATATTGCTCAGGCTTATGATAAGCC
  CCGCTTCACTTTGCACTACAAGACGGACGCACCACTCAACATCCGCAGCATCTTCTATGTGCCAGAGATG
  AAACCATCCATGTTTGATGTGAGCAGGGAGCTGGGCTCCAGCGTGGCACTGTATAGCCGCAAGGTCCTCA
  TCCAGACCAAGGCTGCAGACATCCTGCCCAAGTGGCTGCGCTTCATTCGAGGTGTGGTGGATAGTGAGGA
  CATTCCCCTGAACCTCAGCAGAGAGCTCCTGCAGGAGAGTGCGCTCATCCGGAAACTCCGGGATGTTCTA
  CAACAGAGATTGATCAAGTTCTTCATTGACCAGAGTAAAAAAGATGCTGAAAAATACGCAAAGTTTTTTG
  AAGATTATGGCTTGTTCATGAGGGAGGGCATTGTGACCACTGCAGAGCAAGACATCAAGGAGGATATTGC
  AAAACTGCTACGGTATGAGTCCTCAGCCCTGCCTGCTGGGCAGCTGACCAGCTTACCAGACTATGCCAGC
  CGAATGCAGGCTGGCACCCGCAACATCTATTACCTGTGTGCCCCTAACCGTCACCTGGCTGAACATTCAC
  CCTATTACGAAGCCATGAAGCAGAAACATACTGAGGTGCTCTTCTGCTATGAGCAGTTCGATGAGCTTAC
  TCTGCTGCACCTGAGGGAGTTTGACAAGAAGAAGCTCATCTCTGTGGAAACAGACATCGTCGTTGATCAC
  TACAAGGAGGAAAAGTTTGAGGACACATCTCCAGCTGATGAGCGCCTCTCGGAGAAGGAAACAGAAGATC
  TAATGGCGTGGATGAGAAATGCACTAGGGTCCCGTGTCACCAATGTGAAGGTGACTTTCCGCCTAGACAC
  CCACCCTGCCATGGTGACCGTGCTGGAGATGGGGGCTGCTCGGCATTTCTTGCGTATGCAGCAGCTGGCC
  AAGACCCAGGAGGAACGTGCCCAACTGCTACAGCCCACACTGGAGATCAACCCCAGGCACACACTGATAA
  AGAAGCTCTGCCAGCTGAGGGAGAGCGAGCCGGAGCTGGCCCAGCTGCTCGTGGATCAGATCTATGAGAA
  TGCCATGATAGCAGCAGGACTCGTTGATGACCCCCGGGCCATGGTCGGCCGCCTGAACGACCTTTTGGTC
  AAGGTCCTGGAGAAACACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026508
• Insert Size: 2121 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC022912, AAH22912
• RefSeq Size: 2297 bp
• RefSeq ORF: 2121 bp
• Locus ID: 68015
• UniProt ID: Q9CQN1
• Cytogenetics: 16 2.38 cM
• Gene Summary: Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, downstream of PINK1 and mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA.[UniProtKB/Swiss-Prot Function]