Ankrd13b (NM_172945) Mouse Untagged Clone

CAT#: MC220627

Ankrd13b (untagged) - Mouse ankyrin repeat domain 13b (Ankrd13b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ankrd13b
• Synonyms: AW124583; B930093C12Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220627 representing NM_172945
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCCCCGCCAACGCCTCCGCCAGGAAGGGGCCCGAGGGCAAGTACCCGCTGCACTACCTCGTGTGGC
  ACAACCGCCACCGGGAGCTGGAGAAGGAGGTCCGCGCCGGCCAGGTGGACATTGAACAACTAGATCCCCG
  AGGCCGGACTCCTCTGCACCTGGCTACCACCCTGGGGCACCTGGAATGTGCCCGTGTGCTCTTGGCACAT
  GGTGCAGATGTGGGCAGGGAGAATCGCAGTGGTTGGACAGTGCTGCAGGAGGCTGTGAGCACCAGGGACC
  TTGAGCTGGTGCAGCTGGTGCTGCGGTACAGGGACTACCAGCGGGTGGTGAAGCGACTGGCGGGTATCCC
  CATGCTCCTGGAGAAGCTACGAAAGGCCCAGGATTTCTATGTGGAGATGAAGTGGGAGTTCACAAGTTGG
  GTGCCTCTGGTATCCAAGATTTGCCCTAGTGACACATACAAAGTGTGGAAGAGTGGGCAGAACCTGAGGG
  TAGACACCACGCTTTTGGGCTTTGACCATATGACCTGGCAGAGGGGGAATCGCAGCTTCGTCTTCAGAGG
  TCAAGACACAAGTGCTGTGGTCATGGAGATTGACCATGACCGCCGCGTGGTGTACATGGAGACCCTGGCG
  CTGGCCGGGCAGGATCGGGAGCTACTGCTGGCTGCCGCCCAGCCCTCGGAGGAACAGGTGCTGAGCAGGC
  TTACTGCGCCCGTTGTCACCACACAGCTCGACACCAAGAACATCTCCTTTGAGAGGAACAAGACTGGCAT
  TCTGGGCTGGCGCAGTGAGAAGACAGAGATGGTGAACGGGTACGAGGCCAAGGTATACGGGGCCTCCAAT
  GTGGAGCTCATCACCCGGACACGGACAGAGCATCTTTCGGAACAACATAAAGGCAAAGTCAAAGGTTGTA
  AGACACCTCTGCAGTCTTTCCTGGGAATTGCCGAGCAGCACGGGGGACCCCAAAATGGGACCCTGATCAC
  TCAGACTCTGAGCCAAGCCAACCCCCCTGCCATCACTGCAGAGGAATACTTCAACCCCAACTTTGAGCTT
  GGCAACCGGGCCATGGGACGTCCCATGGAGCTGACCACCAAGACGCAGAAGTTCAAGGCCAAGCTGTGGC
  TGTGTGAGGAGCACCCCCTGTCCCTGTGTGAGCAGGTGGCTCCCATCATTGACCTCATGGCTGTCAGCAA
  CGCACTTTTTGCCAAGCTCCGGGACTTCATCACGTTGCGCCTGCCTCCTGGCTTCCCAGTCAAGATTGAA
  ATCCCGATTTTTCACATCCTCAACGCCCGCATCACCTTCGGGAACCTCAATGGCTGCGATGAGCCAGTGC
  CCTCGGTGCGAGGTAGCCCCGGCAGCGAGACGCCCTCCCCTGGCAGTGACTCTTCCAGCGTCAGCAGCTC
  CAGCTCTACCACCTCCTGTCGTGCCTGTGAGATCTCCCCTGCACTGTTCGAGGCCCCACGTGGCTACAGT
  GTGCTGGGCGGCCAGCGAGAGGCTGTGCCTCGTGATGAAGATGATGACCTGCTGCGGTTTGCCATCCAGC
  AGAGCCTGCTTGAGGCGGGCAGTGAGTATGACCAGGTCACCATCTGGGAGGCGCTAACCAACAGCAAGCC
  AGGCACCCACCCCATGTCCTATGAAGGCCGCCGACAGGACAGGCCCAGAGAACCCTCAGTGCCTTCAACA
  CCTGGTATCGAGAACCCGTTATCAAGATGGCTTCTGCCAACCCCCTTTCCTGGCACAGGGAGGGTGGCCA
  GTGCTCCAGGCATGTGCAGAGATCAGGGACCTAGCTCCCTGGACCTTCAGACCCTCAACAACAGACAGGT
  GTCCCAGGTGTGCCGGGAGCGGGGAGCGCTCGACAAATCCTCCCCGCCCCCAGGAGCGCCCCACCCACGC
  CGCAGCGCCAGCCCATGCCCCCGGCTCCAGTGCCCAGCCCTCGGCCCAGCCCAGGTCCCGGCTCCAGCAG
  CCATGTGTTCCGGAGCTACGACGAGCAGCTGCGGCTGGCTATGGAGTTGTCCGCTCAGGAGCAGGAGGAG
  AGGCGGCGGCGAGTGCGCCAGGAGGAGGAAGAGTTGGAGCGGATCCTGCGGCTCTCGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172945
• Insert Size: 2091 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172945.2, NP_766533.2
• RefSeq Size: 3336 bp
• RefSeq ORF: 2091 bp
• Locus ID: 268445
• UniProt ID: Q5F259
• Cytogenetics: 11 B5
• Gene Summary: Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Does not bind 'Lys-48'-linked ubiquitin. Positively regulates the internalization of ligand-activated EGFR by binding to the Ub moiety of ubiquitinated EGFR at the cell membrane (By similarity).[UniProtKB/Swiss-Prot Function]