Arhgap22 (NM_153800) Mouse Untagged Clone

CAT#: MC220625

Arhgap22 (untagged) - Mouse Rho GTPase activating protein 22 (Arhgap22), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arhgap22
• Synonyms: B230341L19Rik; p68RacGAP; RHOGAP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220625 representing NM_153800
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCGACGGCTTCCAGCAAAAGAAGAACCTTCGCTGCCAGATACTTCACTCGCTCGAAGAGCCTGG
  TGATGGGAGAACAGAGCCGGAGCCCAGGGAGGCCCCTGGTCCCCCACAAGCTGGGCCCTGTACTCAAGGC
  CGGCTGGCTGAGGAAGCAGAGGAGTATCATGAAGAACTGGCAGCAGCGATGGTTTGTGCTACGAGGGGAT
  CAGCTCTTCTACTACAAAGACAAAGATGAAAGCAAGCCCCAGGGATTTATTTCTCTACAAGGGACACAAG
  TGACCGAACTTCTTCCAGACCCTGAGGACCCAGGAAAGCACCTGTTTGAGATTACGCCAGGTGGTGCCAC
  AGAGCGGGAGAAGGTGCCAGCCAACCCGGAGGCGCTCCTGCTCATGGCCAGCTCCCAGCGGGATATGGAG
  GACTGGGTGCAGGCCATCCGCCGTGTCATCTGGGCCCCGCTGGGCAGAGGGATCTTTGGCCAGCGGCTAG
  AGGACACAGTGCACCATGAGCGGAAGTTTGGTCCCCGGCTGGCACCTCTGCTGGTGGAGCAGTGTGTGGA
  CTTCATCCGGGAGCGAGGACTCAGTGAAGAGGGCCTCTTCCGGATGCCTGGCCAGGCCAACCTGGTGAGG
  GACCTGCAGGATTCCTTCGACTGTGGGGAGAAGCCGCTGTTTGACAGCACCACAGACGTGCACACAGTGG
  CCTCCCTGCTGAAGCTCTACCTTCGGGAGCTCCCAGAGCCCGTCATCCCTTTCGCCAGGTATGAGGACTT
  CCTCAGCTGCGCCCAGCTGCTCACCAAGGACGAGGGGGAGGGGACTGTAGAGTTGGCTAAACAAGTGAGC
  AACCTTCCTCAGGCCAACTACAACCTGCTCAGATACATCTGCAAGTTTCTGGATGAAGTGCAGGCCCACT
  CAGATGTCAATAAGATGAGTGTCCAGAACTTGGCAACTGTCTTTGGTCCTAACATTCTTCGGCCACAGAT
  AGAGGATCCAGTAACCATCATGGAAGGCACGTCTCTGGTTCAGCATCTGATGACTGTCCTCATCCGCAAA
  CATGGACAGCTCTTTGCTGCAACCTCCCTTGAAGAACCTGCTTCACCACATGGCACAGTAGAGTGGGGCT
  CTGAAGAGGTCACCAGGGATCACCGGGGTGAGCCTGGCAGCCCTGGCTTGCCCACACATAGGACTTCATC
  TCTGGATGGGCCAGCTGCAGCAGTGCTATCTAGAACTTCGCCGCCGCGCCTTGGCAGCCAAACTGGCCCC
  GCAGCCACCAGCCCTGGGAAGAAGATGCACACTTTGCCTGTCTGGAAGTCTTCTTTTCGGCAGCAGGGAT
  CCCGGTCAGAGAGCCCCAAAGGGGTCAACTCATCCTTAGAGGTACCCATCATCTCCTCTGGTGGGAACTG
  GCTCATAAACGGGCTATCGTCTCTTCGCAGCCACCGTCGGGCCTCATCGGGGGACCGCCTTAAAGATACA
  GGCTCGGCACAGAGACTCTCCACCTATGACAATGTGCCGCCATCTAGCCAATTTTCTAGCACTGCCAGCG
  TGGCCAGCACGTCATGGTCTGTAGCCTCGTCCTCCCGAGAGGCCTCTGTCAGCAGCTGCACAGCCTGCCG
  GGCCAGCAACTCTTCTGCCTGCAGCTCCTTACACACCGAGTGGGCTCTCGAGCCCTCTCCTCTCCCCAGC
  AGCAGTGAGGGCCACCAGTCCCCGGACCTGGGCCACAGCCTGGATGAGCCATGTGTGGGCAGTGGCAGCA
  GTGAGCCCAATGACCCAGGCAGCCCCACACAGGCCCATGTTCGCCGCTGCCGGGCTCTCCAGGGCCAGGT
  GGCCGAGCTCAGGGCAGAGTTGTGTCAGCAGAGGACTGAGTACAAGAGGAGCCTGAAAAGCATCGAGGAG
  GGCAGTGCTGACCTGCGGAAGCAGATGTCTCGGTTGGAAGAGGAGCTGGACCAGGAGAGGAAGAAGTATG
  CCATGCTGGAGATCAAGCTCCGGAACTCGGAGCGAGCCCGGGAGGATGCGGAGCGCAGGAACCAGCTGCT
  GCAGAGGGAGATGGAGGAGTTCTTCTCCACCCTGGGGAGCTTGACTACAGGGACCAAGGGGTCCAGAGCC
  CCGGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153800
• Insert Size: 2109 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153800.4, NP_722495.3
• RefSeq Size: 2598 bp
• RefSeq ORF: 2109 bp
• Locus ID: 239027
• UniProt ID: Q8BL80
• Cytogenetics: 14 B
• Gene Summary: Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. Acts as a GTPase activator for the RAC1 by converting it to an inactive GDP-bound state. Inhibits RAC1-dependent lamellipodia formation. May also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter.[UniProtKB/Swiss-Prot Function]