P3h2 (NM_173379) Mouse Untagged Clone

CAT#: MC220615

Leprel1 (untagged) - Mouse leprecan-like 1 (Leprel1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: P3h2
• Synonyms: 4832416N06; AW553532; Leprel1; Mlat4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220615 representing NM_173379
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGAGAGCACGTGGGTGTCGCTGCTGTTGCTGCTACTGCTGCCCACACCGCAGCGGGGCGGCCCCC
  AGGACGGTCGCCGGTCCCCGGAGCCAGAGCCCGAGCGCGGCCCGCTGCAGCCCTTCGACCTGCTCTACGC
  CAGCGGCGTGGCTGCCTACTACAGCGGCGACTATGAGCGAGCGGTGCGGGACCTGGAAGCCGCCCTGAGC
  AGTCACCGGCGCCTACGGGACATCCGCACGCGCTGCGCCCGCCACTGCGCGGCGCGCCGTCCGCTCGCGC
  CCCCCGGCGCCGGCCCCGGAGCCGAGCTGCCCTTCTTCCGCGCGGTGCTGGAGCGGGCGCGCTGCTCCCG
  CAGCTGCCAGAGCCAGCGCCTGGGCGGCCCCGCGTCCCGCCACCGCGTCAGCGAGGACGTGCGCAGCGAC
  TTCCAACGCAGAGTTCCCTACAACTACCTGCAGAGGGCCTACATCAAGCTCAACCAGCTGGAAAAGGCCA
  TGGAAGCAGCTCACACGTTCTTCATGGCCAACCCCGAGCACATGGAAATGCAGCAGGACCTAGAGGACTA
  TAAAGCCACAGCACGAGTGGAAGCCCCCCTGCTAGACCGCGAAGCCAAGCCACACCTGGAAAGCTATAAT
  GCTGGAGTCAAACATTATGAAGCTGATGACTTTGAATCCGCAATCAAGTACTTTGAACAAGCTTTAAGAG
  AGTATTTCAATGAAGACATGGAGTGCCGTGCTCTATGTGAGGGGCCTCAGAGATTTGAGGAATATGAGTA
  CTTAGGGTATAAAGGTGGTCTTTACGAGGCAATCGCAGATCACTATATGCAGGTTCTGGTCTGTCAGCAT
  GAATGTGTGAGGGAACTCGCCACCCGCCCAGGCCGCCTCTCACCAATCGAGAACTTTCTTCCCTTGCATT
  ACGACTACCTCCAGTTTGCCTACTACAGAGTTGGTGAATATGTGAAAGCCTTGGAGTGCGCCAAAGCCTA
  CCTGATGTTCCATCCAGACAATGAGGATGTCCTGGACAATGTAGACTTCTATGAGAGTCTGCTGGATGAC
  AGCACTGATCCAGCATCCATTGAGGCCCGGGAGGATTTAACAGCATTTGTCAAACGTCATAAACTTGAAG
  CTGAACTGATCAAGTTAGCTGCAGAGGGCCTGGGATTTTCATATGCTGAACCGAATTACTGGATCAGCTA
  TGGAGGACGGCAGGATGAGAACCGAGTCCCTTCAGGAGTGAATATGGATGGGGCAGAAGTTCATGGATTG
  TCAATGGGAAAGAAGTCACCGCCCAAGATAGGTCGAGACCTAAGAGAAGGTGGCCCTCTGCTCTACGAGA
  ACATCACCTTCGTCTACAACTCTGAGCAGCTGAACGGGACTCAACGGGTTCTTCTCGATAATGTCCTGTC
  GCAAGAGCAGTGTCGGGAGCTGCACAGTGTGGCCAATGGAATCATGCTGGTTGGTGATGGATACCGAGGG
  AAAACGTCACCCCACACACCCAATGAGAAGTTTGAAGGAGCAACTGTTTTAAAAGCACTCAAATTTGGTT
  ATGAAGGCCGGGTGCCACTGAAGAGTGCAAGACTGTTCTATGACATCAGCGAGAAGGCTCGGAAGATTGT
  GGAATCCTATTTCATGCTGAACTCAACCCTGTATTTTTCCTATACACACATGGTCTGCAGAACAGCCCTG
  TCTGGGCAGCAGGATCGAAGAAATGACCTCAGTCACCCCATTCATGCAGACAACTGCCTGCTGGATCCAG
  AAGCCAATGAATGCTGGAAGGAGCCTCCCGCTTACACCTTTCGGGACTACAGTGCTCTCCTGTATATGAA
  TGACGACTTTGACGGAGGGGAATTCATCTTCACAGAAATGGATGCTAAAACCGTGACTGCCTCTATAAAA
  CCAAAGTGTGGCCGAATGATCAGCTTCTCGTCTGGCGGCGAGAACCCTCATGGCGTGAAGGCGGTCACCA
  GAGGCCAGCGCTGTGCTGTGGCACTATGGTTTACACTGGACCCTCTTTACCGAGAACTGGAGCGGATACA
  GGCAGATGAAGTGATTGCCATCTTGGATCAAGAACAGCGCGGGAAGCATGGACTGAATATTAACCCCAAA
  GATGAGCTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_173379
• Insert Size: 2112 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173379.2, NP_775555.1
• RefSeq Size: 2248 bp
• RefSeq ORF: 2112 bp
• Locus ID: 210530
• UniProt ID: Q8CG71
• Cytogenetics: 16 B1
• Gene Summary: Prolyl 3-hydroxylase that catalyzes the post-translational formation of 3-hydroxyproline on collagens (PubMed:24368846, PubMed:25645914). Contributes to proline 3-hydroxylation of collagen COL4A1 and COL1A1 in tendons, the eye sclera and in the eye lens capsule (PubMed:25645914). Has high activity with the type IV collagen COL4A1, and lower activity with COL1A1. Catalyzes hydroxylation of the first Pro in Gly-Pro-Hyp sequences where Hyp is 4-hydroxyproline. Has no activity on substrates that have proline instead of 4-hydroxyproline in the third position (By similarity).[UniProtKB/Swiss-Prot Function]