Capn8 (NM_130890) Mouse Untagged Clone

CAT#: MC220609

Capn8 (untagged) - Mouse calpain 8 (Capn8), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Capn8
• Synonyms: nCL-2; nCL-2'
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220609 representing NM_130890
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCCTGGCAGCTGGAATATCCAAGCAACGTGCAGCTGCTCAGGGCCTTGGCTCTAACCAGAATG
  CTGTGAAGTATCTGGGCCAGGACTTCGAGACCCTGAGAAAGCAATGCTTGAACTCAGGGGTCCTATTTAA
  AGACCCGGAATTCCCGGCATGTCCGTCAGCTTTGGGCTACAGGGATCTTGGACCAGGCTCTGCAGAAACT
  CAAGGCATCATATGGAAGCGACCCACGGAATTGTGTTCCAACCCTCAGTTTATCGTTGGTGGAGCCACAC
  GCACAGACATCCGCCAGGGGGGTCTTGGAGACTGTTGGCTTCTGGCAGCCATTGCCTCCCTCACCTTGAA
  TGAAAAGCTGCTTTATCGAGTGGTTCCTAGAGACCAGAGCTTCCAGAAGAACTATGCTGGCATTTTTCAT
  TTCCAGTTCTGGCAGTATGGAGAGTGGGTGGAGGTGGTCATTGATGACCGGCTCCCCACCAAGAATGGAC
  AGCTGCTCTTCCTACACTCCGAAGAAGGCAATGAGTTCTGGAGTGCTCTGCTGGAGAAAGCCTATGCCAA
  GCTCAATGGTTCGTATGAGGCTCTTGCTGGAGGCTCCACAATTGAGGGGTTTGAGGATTTCACGGGTGGC
  ATCTCTGAGTTTTATGACTTGAGGAAGCCTCCGGGCAATCTGTACTACACCATCCAGAAGGCCCTGCGCA
  AAGGCTCTCTGCTGGGCTGCTCCATTGATGTCTCAAACGCAGCCGAAGCAGAAGCCACCACCAGGCAAAA
  GCTGGTTAAGGGCCACGCATACTCTGTGACTGGAGTCGAAGAGGTGGATTTCCGTGGCCTCCCAGAGAAG
  CTGATCAGGTTGAGGAACCCGTGGGGTGAAGTGGAGTGGACGGGAGCCTGGAGTGATAGTGCACCTGAGT
  GGAATTACATAGATCCACAGAAGAAGGGAGAACTGGACAAGAGAGCAGAAGATGGGGAGTTCTGGATGTC
  CTTTTCGGATTTCTTGAAGCAGTTCTCCCGACTAGAGATATGCAACCTGTCCCCAGACTCTCTGAGCAGT
  GAGGAGATACACAAATGGAACCTGGTACTCTTCAACGGCCGCTGGACACGGGGTTCTACAGCCGGGGGCT
  GCCAGAACTACCCAGCCACTTACTGGACCAACCCCCAGTTTAAAATCCATTTGGACGAGGTGGATGAGGA
  CCAGGAGGAGGGCACCAGTGAACCCTGCTGTACAGTGCTGCTGGGTCTGATGCAGAAGAATCGCAGACGT
  CAGAGGAGGATCGGCCAGGGCATGCTCAGCATAGGCTATGCTGTCTACCAGATTCCCAAGGAGCTGGAGA
  ATCACACCGATGAGCACCTGGGCCGGGATTTCTTCCAGGGCCGCCAGCCCTCCACCTGCTCCAGCACCTA
  CATGAACCTTCGAGAGGTGTCCAGCAGGGTCCAACTCCCCCCAGGACAGTACCTGGTGGTGCCATCCACC
  TTTGAGCCCTTCAAGGACGGTGACTTCTGCCTGAGGGTGTTCTCGGAGAAGAAGGCCCAGGCTCTAGAAA
  TTGGGGACGCTGTTGCTGGAGACCCACATGAGCCACATCCCCGTGACATGGATGGAGAAGATGAGCACTT
  CTGGAGCCTGTCTGAGGAGTTTGCGGACAAGGATTCTGAAATCAGTGCTCACCAGCTCAAGAGGGTCCTG
  AATGGATTGCTTTCTAAACGAACAGACATGAAATTTGACGGATTCAACATCAACACTTGCAGAGAAATGA
  TCAGCCTGCTGGATGGTGATGGGACAGGAAGCCTGCGACCGGTGGAGTTCAAGACTCTCTGGCTGAAGAT
  CTGCAAGTATCTGGAGATCTACCAAGAAATGGACCACAGCCGTGCAGGGACCATTGATGCTCACGAGATG
  AGGACAGCTCTCAAGAAAGCAGGTTTCACCCTCAACAACCAGGTGCAGCAGACCATTGCCACGAGGTATG
  CATGCAGCAAGCTTGGTGTTGACTTTGACGGCTTTGTGGCTTGTATGATCCGCCTGGAGATCCTGTTCAA
  ATTGTTCAGACTCTTGGATAAGGACCAGAATGGCATTGTACAGCTCTCCCTGGCTGAGTGGCTGTGCTGT
  GCACTGGTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_130890
• Insert Size: 2112 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130890.2, NP_570960.2
• RefSeq Size: 2411 bp
• RefSeq ORF: 2112 bp
• Locus ID: 170725
• UniProt ID: Q91VA3
• Cytogenetics: 1 H5
• Gene Summary: Calcium-regulated non-lysosomal thiol-protease (By similarity). Involved in membrane trafficking in the gastric surface mucus cells (pit cells) and may involve the membrane trafficking of mucus cells via interactions with coat protein. Proteolytically cleaves the beta-subunit of coatomer complex.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).