Fbxo34 (NM_001146085) Mouse Untagged Clone

CAT#: MC220602

Fbxo34 (untagged) - Mouse F-box protein 34 (Fbxo34), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fbxo34
• Synonyms: 2900057B08Rik; 5830426G16Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220602 representing NM_001146085
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCTTAAGCTGTACTGGAAACTCCAGAAGAAAGAGCGCCCTCTTGAGGTCAGCAGGGACACCCTGA
  GAACGCCTATGAGCCACGGAAAGGCCAACGGTGATGTGAAGGCCAGAGCCAGCTACATGAAACCCACCGT
  CCTTCCTTCAGCCTCCCTTGTCAAAGCATCGTCTAGAAAACCTTTTGGGATCCTTTCTCCAAATGTTCTG
  TGCAGTATGAGTGGGAAGAGCCCTGTGGAGAACAGCTTGAATGTTAAAGCCAAAAAGAACGTGCTGTCTG
  CAGCCGTGCACCAGAGTGAGGAGGGGCTGCCTGGGACCTGGGCTATTGTCAAACCTGGGAACACGAAGGA
  GAAAATTGCATTCTTTGCAGCCCACCAGTATAGCAATAGGATAGGCTCTATGAAAATTAAAAGTTCCTGG
  GATATCGATGGGAGAGCCACTAAAAGAAGGAAAAAATCAGGAGATCTTAAGAAAGCCAAGTTACAGCTAG
  AAATGATGAGAGAGATCAACAGCCAGTGCTACCAGTCTGAGCCGTTTGTGTGTGGCGTGGAGCACTGTTC
  TGTGCACTACATGAGTGACAGCGGGGACGGTGTCTGTGCTGCGAGGCCTCTGTCGGTGATACAGATGGTT
  GCCTTCCTTGAGCAGAAGGCCACTGCTCTGCTAGCTAGCTGCACAAAAAACTGCACAAATTCGCCTGCCA
  TTGTGAAGATTTCTGGCCAGTCCCGAGGTATACCGCCTGCACCTGAGCCCTTCTCTGCTCCGGAAACTTG
  TGAAGAACCCAAGGAACAAGAAAATCCCGAGACTGGCAGATCCCAGGGTGAGCCAGTCCGTGTCCTTGAC
  ATGGTAGCCAGGTTGGAGTCTGAGTGCCTGAAGCACCAGGGCCAGCGGGAACCCGGGAGCTTGTCACGCA
  ATAACAGTTTCCGTCGAAATGTAGGCAGGGTGTTGCTCACTAATGGTTCTCAGGCCAGCGACAAAAGTGA
  AGAGGGCTCTGCAGACACAGCCGACCCTCAGGAGAATCCCTTGCAGCCGGTATCTGTGGGTGAAGAACCC
  TCCGTAACTGAGCATCATTCTGTAGGGGAGCAGGCCTGGGATGGCACTTCTCAAAGCTGTCCCTCATTGC
  CAGCCACTGTGAGCTTCCACATGGACAGCACAGATCTAGAGCCGGGTCAGCAAACAGCTATGAAGAGCTG
  CAGCCGGGATGACGTAGAAATGGTGGAGGAATTTGATGAATTGCCTACAGATGCTGTCCGTCGCATAAGA
  AGAGAGCTTGTAACAGTCACAAAGCACAGTCCTGAGCAGAGGCAGGACCCGCTGTGCATCAGCATCACTG
  TGTGCACGGTAGAGAAAGACCGGCCGGCGGCTTTAGACTCCCTTGAGGAGCCTCTTCCCGGGATGCTCTT
  CTTTCTGTCATCTGGACAGGACCAGCAAGCGCACCCCCAGCTGAGGGAACACCCAGCACCAGAGGCCTCA
  GAGGCCAGTCAGCCTCAAGATGCTGCTGAGGGCAGCAGTGCAGGTGAGGAGAAAGATGCCTCTGTGGAGC
  CGCTCCTCCCAGCAGCCTCTCCGGGAGGGAGCACCTCGCAGGTGCTTGAGGCAGCCACGTGCAAGAAGCA
  GGTGTCACAGGACTTTCTGGAGACCAGGTTTAAGATCCAGCAGCTTCTGGAGCCTCAGCAGTACATGGCA
  TGCCTGCCCCACCACATCATAGTGAAAATCTTCAGGTTACTCCCTACCCTGAGTTTAGCCATTCTTAAAT
  GTACCTGTCGCTACTTCAAGTCCATCATTGAATACTACAACATCAGGCCAGCAGATTCTCGGTGGGTTCG
  AGACCCACGCTATAGGGAAGACCCTTGCAAGCAGTGCAAGAAAAAGTATGTGAAAGGGGATGTGTCCCTG
  TGCCGGTGGCACCCCAAACCCTATTGCCAGGCTTTGCCATATGGGCCTGGCTACTGGATGTGCTGCCACC
  AGTCTCAGAAGGGCTTTCCTGGCTGTAAGTTAGGCCTTCATGACAATCACTGGTTACCTGCCTGCCATAG
  CTTTAACCGGGCAATCCATAAGAAGACGAGAGGGAGTGAGACGGAAGAGGAGTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001146085
• Insert Size: 2088 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146085.1, NP_001139557.1
• RefSeq Size: 3132 bp
• RefSeq ORF: 2088 bp
• Locus ID: 78938
• UniProt ID: Q80XI1
• Cytogenetics: 14 C1
• Gene Summary: Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) includes two alternate segments which result in the use of a downstream start codon, compared to variant 1. Variants 2 and 3 encode the same protein (isoform 2) which is shorter when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.