Nol11 (NM_001161329) Mouse Untagged Clone

CAT#: MC220590

Nol11 (untagged) - Mouse nucleolar protein 11 (Nol11), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nol11
• Synonyms: 1500002M01Rik; AU015220; AU019874
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220590 representing NM_001161329
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAACCCTGGAAGAAGAATTCACGTTGTCTACTGGAGTCTTAGGCGCCGGGCCTGAGGGATTCCTAG
  GTGTAGAGCAGACTGACAAAGCCGACCAGTTCCTAGTGACGGACAGCGGCAGGACCGTCGTCCTTTACAA
  GGTTTCTGACCAGAAACCGTTGGGCAGCTGGTCGGTGAAGCAAGGTCAGAGTATAACATGCCCTGCTGTG
  TGCAACTTTCAGACTGGAGAGTATATTATGGTACATGACCATAAGGTTTTGAGAATATGGAATAATGATG
  ACGTAAACGTGGATAAAGTATTCAAAGCTACACTGTCAGCTGAAGTCCATAGAATCCACTCAGTACAAAG
  AACAGAACCCTTGGTGCTGTTCCGAGGAGGCGCTGCTCGTGGGCTAGAGGCCTTGCTTGTAGAGCCCCAG
  CAGAACATCGAATCTGTCATACCTGATGAGGAAGTGATCGTGTGGTCAGAGGTTTTCATGTTATTTAAGC
  AACCAGTTTTAATTTTTATTACTGAAAATCATGGTCATTATTATGCTTATGTACGATTATGCAAATCACA
  CAGCTTAAGCAAATACACACTCTTACTTGAAAAAGAAGAAAAATCTGTTAAACCAAATTTTACTGCACGT
  GTGGATGGGAAATTCATCTCCCTGGTGTCGCTAAGCTCTGATGGGTGTATATATGAAACCTTGATACCAA
  TATATTCAAGTGACACAGAACAAAACCAGAGGTTAGTTAGAGCATTGATGCTCAAGTCAGTTGTGTCTGG
  CGGTGTTCGAAATGGTGTTGCCCTCACCATCCTGGATCAAGACCACATAGCTGTCCTGGGACCTCCACTT
  TCAGCTTCTAAGGAGTGCCTCTCCATATGGAACATAAAGTTTCAAACATTACAGACGTCAAAAGAGCTGC
  CACAAGGAACCAGTGGGCAGCTCTGGTATCATGGGGAAGTACTATTCATGCTACATGGAAAAAGTCTAAC
  TGTGATTCCATACAAGTGTGAAGAATCGTCTCTAGCAGGCGCTCTTGGAAAACTCAAGCACACACAAGAG
  TCAGGCACTCATTCTGTGCCCCACTTTGTAAACTGGGAAACATGTTCAGGATATGAACTTGGGTCCTCCG
  GTGCAGAGCAGTCAAGAACTCTTAGGAGAAAAAAAGTTGAAACAAATTTACAGCCAGAAGTTCCAGGATT
  CAAACAACTTTTATCAATAATAAAGAAAGATTCAGAAAAGCACATTGAAGTAGAACTCCGTAAGTTTTTG
  GCGAAGTCAACACCTGCCTTTCATACTATAATTGGAGACTTAGTAGCAGGGCTTGTGGGAAGATGTAAAG
  CCGAGCCATCGTTTTACCCCCGGAACTGTCTGACACAGCTCATCCAAACACACGTGCTTTCCTACAGCTT
  ATGCCCTGACTTGATGGAGATTGCCCTAGAGCACACAGATGTGCAGATGTTACAGCTGTGTCTACAGCAG
  TTCCCTGACATTCCTGAGTCCACCACCTGTGCTTGCTTAAAACTTTTCTTGAGTGATTCCACACAAGATG
  AGAAAAAGGAAATGGAAGAGCAAATTGAAATTGTTCAGAATGGCTTTGGACCTGAAGATGGGAACTGCAG
  TGAAGATAGTCAGCAGTTAAATGATAAGCCTGCAGACACAGCACACGAGCCCGGCTCCTTCCCTGTGACC
  TCATGTCCTGTGGCACCAAAGCGAGCAGCTCTGCTAAATGCAGTCCTTCATTCCGCATACAGTGAGCCCT
  TCCTCCTGCCGCACTTGAAGGACATCCCTGCGAAGCATGTCACGCTGTTTCTCCAGTATTTGTATTTCCT
  CTATTTGAAGTGTACTGGCAGTGCTACCATGACTCTCCCTGGAGTGAACCCTCCAACCGTGAGCCAGATT
  ATGGATTGGATATGCCTACTTCTAGATGCTAATTTTACTGTCCTATTAATGATACCAGAAGCAAAAAGAC
  TTTTACTTATTCTTTACAATTTTGTGAAATCTCAGATCTCCATCTATTCCGAGCTCAACAAGATCGCAGT
  CAGCTTCCGGGAGCTGCAGAGATTAAATCGGGAGAAGAGCAGTAGAGGACTGTACTCCATCGAAGTGCTG
  GAACTCTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001161329
• Insert Size: 2112 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161329.1, NP_001154801.1
• RefSeq Size: 3108 bp
• RefSeq ORF: 2112 bp
• Locus ID: 68979
• UniProt ID: Q8BJW5
• Cytogenetics: 11 E1
• Gene Summary: Ribosome biogenesis factor. May be required for both optimal rDNA transcription and small subunit (SSU) pre-rRNA processing at sites A', A0, 1 and 2b (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame segment in the CDS, as compared to variant 1. The resulting isoform (2) is shorter but has identical N- and C-termini, as compared to isoform 1.