Alox12b (NM_009659) Mouse Untagged Clone

CAT#: MC220562

Alox12b (untagged) - Mouse arachidonate 12-lipoxygenase, 12R type (Alox12b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Alox12b
• Synonyms: 12R-; 12R-LOX; Alo; Aloxe2; e-LO; e-LOX2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220562 representing NM_009659
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCTATAAAGTCAAGGTGGCCACGGGCACTGACTTCTTTTCGGGGACGCTGGATTCAATTTCAC
  TGACCATCGTGGGGACCCAAGGAGAGAGCCATAAGCAGCGGTTGAACCACTTTGGGAGAGACTTTGCCAC
  GGGGGCGGTGGATGACTACACGGTACAGTGCCAGCAGGACCTGGGGGAGCTTATCATCATCCGTCTGCAC
  AAGGAGCCTCATTCCTTCCTAGCCAAGGACCCCTGGTACTGCAACTATGTGCAGATCTGTGCTCCCGACT
  GCCGTGTCTACCACTTCCCAGCTTACCAGTGGATGGATGGCTATGAGACCCTGGCACTCCGGGAGGCCAC
  AGGAAAGATAACAGCAGACGACACACTCCCTATCCTTTTGGAACACAGACAAGAAGAGATCAGAGCCAAG
  AAGGACTTCTACCACTGGAGGGTCTTTGTTCCTGGCCTTCCCAACTATGTGGATATCCCCAGTTACCATC
  CTCCTCCCCGACGGTGCCGCAATCCCAACCGGCCTGAGTGGGATGGTTATATTCCCGGATTCCCAATTCT
  CATCAACATTAAGGCTACCAGGTTCTTAAACTCAAATCTTCGTTTCTCCTTCGTCAAGACAGCCTCCTTC
  TTCTACCGCCTAGGGCCCATGGCGCTGGCCTTCAAACTGCGTGGCCTGGTGGATCGAAAACGCTCCTGGA
  AGAGACTAAAGGACATTAAGAATATTTTCCCTGCTACCAAGTCTGTCGTCTCAGAGTATGTGGCCGAGCA
  CTGGACAGAGGACAGCTTCTTTGGGTACCAGTACCTTAATGGTATTAACCCAGGCCTGATCCGAAGATGT
  ACACAGATCCCAGACAAATTCCCAGTCACAGATGAAATGGTGGCCCCATTCCTGGGTGAAGGAACATGCC
  TGCAAGCAGAGCTGGAGAGGGGGAATATTTACCTGGCAGACTACCGCATCCTGGATGGCATCCCCACCGT
  GGAGCTCAATGGTCAGCAGCAGCATCACTGTGCCCCGATGTGCTTGCTGCACTTTGGTCCTGATGGCAAC
  ATGATGCCCATCGCCATTCAGCTCAGTCAGACTCCTGGGCCTGATTGTCCTATCTTCCTGCCGAATGATT
  CTGAGTGGGACTGGCTGTTGGCTAAAACGTGGGTGCGCTACGCGGAGTTCTACAGCCACGAGGCCGTGGC
  ACACTTGCTGGAGAGCCACCTCATCGGGGAAGCTTTCTGCTTGGCCCTCCTGAGGAACCTGCCCATGTGC
  CACCCCCTGTATAAGCTTCTCATCCCTCACACTCGGTACAACGTCCAGATCAATAGCATTGGGCGGGCCC
  TCCTTCTCAACAAGGGAGGTCTCTCTGCCAGGGCCATGTCCCTGGGCCTAGAGGGCTTCGCACAGGTGAT
  GGTTCGGGGTCTGTCTGAGCTCACCTACAAAAGCCTCTGCATTCCCAACGACTTTGTGGAGCGCGGGGTC
  CAGGACCTGCCTGGGTATTATTTCCGTGATGACAGCCTGGCGGTGTGGTACGCGATGGAGAGGTACGTGA
  CAGAGATCATCACTTATTATTACCCAAATGACGCAGCCGTAGAGGGCGACCCGGAACTGCAGTGCTGGGT
  ACAGGAGATATTCAAGGAGTGTCTCCTGGGGCGTGAGAGCTCAGGCTTCCCCACCTGCTTGCGAACTATA
  CCTGAGCTGATCGAATACGTCACAATGGTCATGTACACCTGCTCGGCCAGGCATGCAGCTGTCAACTCAG
  GCCAGCTGGAGTACACCTCTTGGATGCCCAACTTCCCATCATCCATGAGGAACCCACCGATGCAGACCAA
  GGGGCTAACTACTCTGCAGACATACATGGACACTCTTCCGGACGTGAAGACCACATGCATTGTGCTCTTG
  GTGCTCTGGACTCTCTGTCGGGAGCCTGATGACAGGCGGCCGCTTGGCCACTTCCCAGACATCCATTTTG
  TGGAGGAGGGTCCTCGGAGGAGTATTGAGGCTTTTCGTCAGAACCTCAACCAGATCTCTCACAACATCCG
  ACAGCGCAATAAGTGCCTCACCCTCCCGTACTACTACCTGGACCCAGTGCTGATTGAGAACAGTATTTCC
  ATCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009659
• Insert Size: 2106 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009659.2, NP_033789.1
• RefSeq Size: 2347 bp
• RefSeq ORF: 2106 bp
• Locus ID: 11686
• UniProt ID: O70582
• Cytogenetics: 11 42.38 cM
• Gene Summary: This gene encodes an enzyme involved in the conversion of arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in this gene can prevent the formation of the epidermal permeability barrier and cause an ichthyosiform phenotype. [provided by RefSeq, Sep 2015]