Dennd1b (NM_181347) Mouse Untagged Clone

CAT#: MC220549

Dennd1b (untagged) - Mouse DENN/MADD domain containing 1B (Dennd1b), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dennd1b
• Synonyms: 4632404N19Rik; 4930467M19Rik; 6820401H01Rik; F730008N07Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220549 representing NM_181347
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAGTAAGCAGCGATTTGGGTTCTGCAGGCTCACATCTGGAGGCAGAATTTGTTTATGTATTCTTA
  GTTACTTGCCATGGTTTGAAGTATATTATAAACTTCTAAATACTCTGGCAGATTACTTGGCCAAGGAACT
  GGAAGAGGATTTGAATGAAACTCTTAAATCACTCTATAACCACCCAGTACCAAAGGCAAACACTCCTGTC
  AACTTGAGTGTGCACTCCTGTTTCATCACACCTGACATAACTGGACTCCCAACGATACCGGAGAGTAGAA
  ACCTGACCGAGTACTTTGTTGCCGTGGATGTCAACAACATGCTGCGACTGTACGCCAGCATGCTGCATGA
  GAGGCGCATCATTATTACCTCGAGCAAATTAAGCACTTTAACTGCCTGTCTCCATGGATCGGCTGCGCTA
  CTCTACCCGATGTACTGGCAACACATATATATCCCCGTGCTTCCTCCACACCTGCTGGACTACTGCTGTG
  CCCCAATGCCATACCTGATTGGAATACACTCCAGTCTCATAGAGAGAGTGAAAAATAAATCATTGGAAGA
  TGTGGTTGTGTTAAATGTTGACACAAACACGTTAGAATCACCATTTAATGACTTGAGCAGCCTGCCCAGT
  GATGTGGTCTCGGCCTTGAAAAATAAGCTGAAGAAGCAGTCCACAGCTACGGGAGATGGAGTGGCTCGGG
  CCTTTCTGAGGGCTCAGGCGGCTTTGTTTGGCTCCTACCGAGATGCTCTGAGATACAAGCCTGGCGAGCC
  CATCACCTTCTGTGAGGAGAGCTTTGTAAAGCACCGCTCAAGCGTGATGAAACAGTTCCTGGAGACTGCA
  GTTAACCTTCAGCTCTTTAAGCAGTTTATTGATGGTCGACTGGCAAAGCTTAACGCAGGAAGAGGTTTCT
  CTGATATCTTCGAAGAAGAGATCACTTCGGGTGGCTTCTGTGGAGGGAGCCCGAGGTCATATCAGCAGTG
  GGTGTATACAGTCAAGAAAGGAGGTGCATTGTTCAACACAGCAGTGACCAAAGCAACTCCCGCTGTGCGG
  ACTGCATACAAATTTGCAAAGAGTCACGCCAGACTGGGACTAAAGGAGGTGAAGAGCAGGCTGAAACACA
  AGGACAATGAGGAAGAGTATGGGACCTGTTCTGGTTTGGTGCAGTATACACCAGTGTACACTTTACACAA
  TGAAAAGGGAGAGAACAGAGAGAAGCACAAGCTTTCCCAGACACACTTGAAAAGGCCTCATAAGAGCCTA
  GATGGTACCCTGTATGATGATGATGATGATGACGATGACATTGAACGGGCAAGCAAGATATCTTCTGAAG
  ACGGGGAAGAAACCCGTGCTTATTTCTACGAAAGCGATGACTCCGTTGAAGCCCAGGTGAAGGCTCCTTA
  CTCAGGGGAGATGGACTTACTGGGTGAGATCCTGGACACGCTGAGCACGCACAGCTCTGATCAAGGAAAG
  CTGGCGCCTGCCAAGAGCCTGGATTTCTTCAGATCCATGGATGACATTGATTACAAGCCTACGAACAAGT
  CCAACGCTCCCAGTGAGAATAACCTGGCCCTGCTGTGTGCTTCTGGAGACCAGGGAGAGTGGAATCTCGG
  GCAGGACGACAGCGCCCTCCATGGCAGGCAACTCCCTCCATCCCCAAGGAAACGCGTTTCCTCTGGTGGG
  CTGACAGAGTCTCTGTTTATCCTGAAAGAGGAGAGCAGGGAGAAGCCCCTCTGTGCTGACAGCGTAAGTG
  GCCCTACTGTGGTGGGAAAGCCAGCTCCGACTTCAGGACTGCGTTCCCAGCCTGCTGCTCCTGAAGCTTC
  CCAAACAGAGAGAGGAAGAGCAGAAGTGAAGCAAACACCAGGTCAGGCTCCACTTCAGTCAGAGGATCTG
  TCGGTGCCCGGCCCCGGGAGCCGCCAGTCGACTTTTGTTCCCTGGGAGAAGGCTGGCAAAGAGGACACGA
  AGCCTTCAAAGGATGTCGGACTTCTCCAAGAAGTAGTGTCATTGTGTCACATGTCATGTGACTTCCAACA
  AGATTTAAACATTTCAGAAGAAAGCAGAAGTGGAAACCAAACTTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_181347
• Insert Size: 2076 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181347.3, NP_851992.1
• RefSeq Size: 8072 bp
• RefSeq ORF: 2076 bp
• Locus ID: 329260
• UniProt ID: Q3U1T9
• Cytogenetics: 1 E4
• Gene Summary: Guanine nucleotide exchange factor (GEF) for RAB35 that acts as a regulator of T-cell receptor (TCR) internalization in TH2 cells. Acts by promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Plays a role in clathrin-mediated endocytosis. Controls cytokine production in TH2 lymphocytes by controlling the rate of TCR internalization and routing to endosomes: acts by mediating clathrin-mediated endocytosis of TCR via its interaction with the adapter protein complex 2 (AP-2) and GEF activity. Dysregulation leads to impaired TCR down-modulation and recycling, affecting cytokine production in TH2 cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR and uses a downstream start codon, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.