Fbxl5 (NM_001159963) Mouse Untagged Clone

CAT#: MC220539

Fbxl5 (untagged) - Mouse F-box and leucine-rich repeat protein 5 (Fbxl5), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fbxl5
• Synonyms: Fbl4; Fir4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220539 representing NM_001159963
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCCTTCCCCGATGAAGTGGACGTCTTCACCGCCCCGCACTGGCGGATGAAGCAGCTGGTGGGGC
  GCTACTGCGACAAGCTTTCTAAAACCAACTTTTCCAACAACAATGACTTCCGTGCTCTTCTCCAGTCTTT
  ATATGCTACTTTCAAGGAGTTTAAAATGCATGAACAAATTGAAAATGAATACATTATCGGTTTACTTCAG
  CAGCGCAGTCAGACCATATATAATGTACACTCTGACAATAAGCTCTCCGAGATGCTGAGTCTCTTTGAAA
  AGGGGCTGAAGAATGTCAAGAATGAGTACGAACAGTTAAATTATGCAAAACAGCTGAAAGAGAGATTGGA
  GGCTTTTACAAGAGATTTTCTTCCTCACATGAAAGAGGAAGAAGAGGTTTTTCAGCCCATGTTAATGGAA
  TATTTTACCTACGAGGAGCTTAAAGATATCAAAAAGAAAGTGATTGCACAGCACTGCTCACAGAAGGACA
  CGGCAGAGCTCCTCAGAGGGCTCAGTCTGTGGAACCAAGCTGAGGAGCGACAGAAAGTTCTCAAGTACTC
  TGTGGATGAGAAGGCAGATACAGAAGCGGAAGTGTCAGAACACTCCACAGGTATAACCCATCTTCCTCCT
  GAGGTAATGCTGTCCATTTTCAGTTACCTTAATCCTCAAGAATTGTGTCGGTGTAGTCAAGTCAGTACTA
  AGTGGTCTCAGCTGGCAAAAACAGGATCTTTGTGGAAACATCTTTACCCTGTTCATTGGGCAAGAGGTGA
  CTGGTATAGTGGTCCTGCCACTGAACTTGATACTGAGCCTGATGAGGAATGGGTGAGAAATAGGAAAGAT
  GAAAGTCGTGCTTTCCAGGAGTGGGATGAAGATGCCGATATAGATGAATCTGAAGAGTCTGCAGAGGAAT
  CGGTTGCTATCAGCATTGCACAAATGGAAAAACGTGTACTCCATGGCTTAATTCATAATGTTCTGCCATA
  TGTTGGTACCTCTGTAAAAACTTTAGTGTTAGCGTACAGCTCTGCAGTCTCCAGCAAAATGGTTAGGCAG
  ATTTTAGAGCTTTGTCCTAACCTGGAACATCTGGATCTTACCCAGACCGACATTTCTGATTCTGCATTTG
  ACAGTTGGTCTTGGCTCGGTTGCTGCCAGAGTCTTCGGCATCTTGATCTGTCTGGATGTGAAAAAATCAC
  AGACATGGCTCTAGAGAAGATTTCTAGAGCTCTTGGAGTTCTGACATCTCATCAGAGTGGCTTTCTGAAA
  AGTGCAGGCAAGGCTGCTTCAACTCCATGGACAAGTAAAGACATTACCATGCCATCCACCACGCAGTATG
  CCTGTTTGCACAATTTAACTGACAAAGGCATTGGTGAAGAAATAGACAACGAGCACTCTTGGACTGAACC
  TGTGTCTTCTGAAAGTTTGACTTCTCCTTATGTGTGGATGCTAGATGCTGAAGATTTGGCTGATATTGAA
  GATGCTGTAGAGTGGAGACACAGAAATGTTGAGAGTCTCTGTGTGATGGAAACAGCCTCCAACTTTGGTT
  GTTCCTCATCTGGTTGTTACAGTAAGGACATTGTGGGATTAAGGACTAGTGTCTGTTGGCAGCAGCATTG
  TGCTTCTCCAGCCTTTGCATATTGTGGTCATTCATTCTGTTGTACAGGGACAGCTTTAAGAACTATGACT
  ACACTCCCAGCGACTTCTGCAATGTGTAGAAAAGCATTAAGGACTACATTGCCCAGGGGGAAAGACTTAA
  TTTATTTTGGGAGTGAAAAGTCTGACCAAGAGACTGGACGAGTACTTTTGTTCCTCAGTCTGTCTGGATG
  TTACCAGATCACAGACCATGGTCTCAGGGCTCTGACTCTGGGAGGAGGGCTGCCTTACCTGGAGCACCTG
  AATCTCTCTGGCTGTCTGACTGTAACTGGTGCAGGGCTGCAGGATTTGGTCTCAGCGTGTCCTTCCCTAA
  ATGACGAATACTTTTACTACTGTGACAACATTAACGGTCCTCATGCTGACACCGCCAGTGGATGCCAGAA
  TTTGCAGTGTGGTTTTCGAGCCTGCTGCCGCTCTGGCGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001159963
• Insert Size: 2073 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159963.1, NP_001153435.1
• RefSeq Size: 2855 bp
• RefSeq ORF: 2073 bp
• Locus ID: 242960
• UniProt ID: Q8C2S5
• Cytogenetics: 5 B3
• Gene Summary: Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (A).