Rnf169 (NM_175388) Mouse Untagged Clone

CAT#: MC220526

Rnf169 (untagged) - Mouse ring finger protein 169 (Rnf169), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rnf169
• Synonyms: 2900057K09Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220526 representing NM_175388
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCAGGTCCGAGCACTCGGGCTTCTTCCGCTGCGGCCGCGGCAGCTCTGAGTCGGCGGGGCC
  GGAGGGGCCGCTGTGACGAGATGGCGGCAGCGAAAGCCGGGGCTCCGGGCCCGGCCTCCAGCCCCGCGTT
  GTTAGTGTTGCGGTCAGCGCCACGGCCGGAGGAGTCGGGCTGCACCGGGTGCCTGGAGACCCCGGGGGAA
  GTGGCGGCCCTGCCGTGCAGCCACTCGCGGTGCCGAGGCTGCGCCTCGCGCGCGGCGGGCCCGGGCTGCC
  GTCGCTGCCGTCCCCGCGGTTCGGGCTGGGCCCGCCGTCGGGCGCGCGACGACGGCCAGGCCGCCGCGGA
  GCTGATGGGCGAGCGCGCCCGTCGTGGACAGCCAGAACCCTGCCGCCCGCGCCGGGACGGGGGCGCGGCT
  GCCTCGGGGCCCAGGCCGGAGCCGGAGCCGCTCGCGGAGCCAGAGTTTATATTCAGAACGCCAATCAAGT
  TAAGCAAGCCGGGAGAACTCAGTGAGGAGTATGGATGCCTGAGAAAGCTGAGAGGGGAGAAGTTACAGGA
  AGAAAAAGATTGTGATGATCAGATACACAAGCTACTGCAGGAGGATTCCGAAATGGGGAAGAGGAAAGCT
  GATGAGCAGAAAAAAAGGGATGAAGCAGTGGTGCTGAAAACAAGTCTGGAGCAGTGTCCTGCGCGTCTCT
  CGGATTCAGAGAATGAAGAGCCTTCTCGGGGCCAGATGATGCAGACCCATCGCTCCGCCTTTGTTTCTAA
  GAACAGCTCCTGCTCCTTAGCGTTCCTGGCAGGGAAGTTAAACACCAAGGTGCAGAGAAGTCAGAGCTGC
  AGCGACACAGTTCAGGACAGAGTGAGGAGCAGGCTCAGAACAGCTCCGCCCAACAGAGCCAAGATCACAA
  CTATAACTCCAGGCTCCACCCCCATTATTGGTGTTCTCTTGTCTACTCAAAACAACCGCTGCCTCTCTGC
  CCCTGACTTAACCATCGAAAAGCGCCTGCCCTTCGGCTCCCTTTCATCCTTGGCTTCCTTGCATAAGCCA
  GAGCGCTCCATCAGCCCTGAAAGCAATGACAGCATCTCTGAAGAGCTAAACCACTTCAAGCCCATCGTCT
  GCTCGCCATGCACCCCTCCCAAGAGACTCCCTGATGGCCGGGTGCTCAGTCCTCTCATCATCAAGTCAAC
  CCCTCGCAACCTGACCAGAAGCCTGCAGAAGCAGACTTCATATGAGGCCAGCCCTCGCATCCTCAAGAAG
  TGGGAGCAGATCTTCCAGGAGCGGCAGATCAAGAAGACCCTTTCCAAAGCCACTCTCACCTCCCTGGCTC
  CTGAAGCAGGGGAAGAGTTTCCAGGTTCTGACACAATCCATTCCAGCAAGGAGAGGCCGTCTCTGGCCTT
  TAATACAAGGCTGTCCAGGGTCCAGGTGCTCTCCGAGTGTGCTGGGCCCACCTCCACTGCCCTTGAGTGC
  TTCCCCTCTGTTAACCAGACCAAAGTAGAACAGGACTGTGTTAGAAAACGGAGCCGTGAGTTCTCACTGG
  AAACCTGCCATTCCTCGGAGCACGGAGGAGCCTCCAGTGGGCCTTCTCTGGAACGGGAGCAGTGTGAGGA
  GTCAGGGTCCACCGTGGACGCCACATTAGTCAAAACTTGCATAAGCACAGTCATGAAGACTGCAGCGGTT
  AATTCACTGCTACCCAAAAATGATGTTTTGGGTGGGGTCCTCAAAACAAAACAACAACTGAAGACACTCA
  ATCATTTTGATCTGGGTAATGGCATTCTGGTCAACAGCCTAGGAGAGGAGCCGATTCCTTCCCTGCGCAG
  AGGCCGGAAAAGGCGCTGTAAGACCAAGCACTTGGAACAAAACGGTGTTAAAAAACTGCGACCACCGAGC
  AGTGACATGGATCTGGCCCCCAAGGACCCAGGGCTGCTTGAGGTGGGGCGGAAATTGCAGCAGGAGGAAG
  AGGACCAGCAGCTGGCTCTGCAGTCACATCGCATGTTTGACAGTGAGAGGCGGACTATGAGCCGGCGAAA
  AGGAAGCGTAGATCAGTATCTCTTGAGGTCCAGCAGCTTGGCTGGGGCCAAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175388
• Insert Size: 2085 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175388.3, NP_780597.2
• RefSeq Size: 7157 bp
• RefSeq ORF: 2085 bp
• Locus ID: 108937
• UniProt ID: E9Q7F2
• Cytogenetics: 7 E2
• Gene Summary: Probable E3 ubiquitin-protein ligase that acts as a negative regulator of double-strand breaks (DSBs) repair following DNA damage. Recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSBs repair. E3 ubiquitin-protein ligase activity is not required for regulation of DSBs repair.[UniProtKB/Swiss-Prot Function]