Prdm8 (NM_029947) Mouse Untagged Clone

CAT#: MC220521

Prdm8 (untagged) - Mouse PR domain containing 8 (Prdm8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prdm8
• Synonyms: 4930565F05Rik; PFM5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220521 representing NM_029947
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGATTCAGGCATCCAGAGAGGCATCTGGGATGGAGATGCCAAGGCTGTCCAACAGTGTCTGACAG
  ACATTTTTACCAGTGTATATACAACCTGCGACATCCCAGAGAACGCCATATTCGGTCCCTGTGTACTGAG
  CCATACTTCCCTGTATGACAGCATAGCCTTCGTAGCCCTCAAGTCCACGGACAAGAGAACAGTACCTTAC
  ATCTTCCGGGTAGACACTTCGGCGGCAAATGGTTCCTCAGAAGGTCTCATGTGGCTGCGGCTGGTCCAAT
  CAGCCCGAGATAAGGAAGAACAGAATCTCGAAGCTTATATAAAAAATGGACAGCTGTTCTACCGCTCTCT
  CCGCAGGATTGCCAAAGATGAAGAGTTACTAGTTTGGTACGGGAAAGAACTGACTGAATTGCTCTTGCTC
  TGCCCGTCTAGAGCTCACAAAATGAACGGGTCGTCTCCTTACACATGCCTGGAATGCAGCCAACGTTTCC
  AGTTTGAGTTCCCCTATGTGGCACATCTGCGATTCCGCTGCCCCAAGAGACTTCACAGCACTGATGCGAA
  TCCCCAAGACGAGCAAGGGGGCGGCTTGGGCACTAAGGACCACGGCGGCGGCGGCGGTGGTAAAGAGCAA
  CAGCAGCAGCAACAGCAGCAGCAACAGGAGGCGCCGTTGATCCCGGGCCCCAAGTTCTGCAAAGCCGGCC
  CCATACACCACTACCCCGCGTCATCCCCGGAGGCGAGCAACCCGCCGGGCTCCGCGGGTGCCGGCAGCGC
  CAAGCCGTCCACGGACTTCCACAACTTGGCTCGGGAACTGGAAAACTCCCGGGGAAGCAGCAGTTGCGTA
  GCGGCCCCGGGCGTCGGCAGTGGCGGCAGCGGCCACCAGGAGGCGGAGCTGAGTCCCGACGGCGTCGCCA
  CCGGCGGCTGCAAAGGCAAGAGGCGGTTCCCAGAGGAGGCGGCGGCGGAGGGCGGCGGCGCGGGGCTGGC
  GGGCGGCCGTGCGCGCTTCTCCGAGAGGCCGCTGGCGACCTCCAAGGAGGAGTTGGTGTGCACGCCGCAG
  CAGTACCGCGCCGCCGGCAGCTACTTCGGCCTGGAGGAGAACGGCCGGCTCTTCGCGCCGCCCAGCCCGG
  AGACTGGCGAGGCGAAGCGCAGCGCCTTCGTGGAGGTGAAGAAGGCGGGCCGCGCGGTGGGCTTGCAGGA
  GGAGGCGGCGGCGACAGACGGCGCAGGGGGCACGGCCGAGGACCCCGACGCGGGAGGCGGTGTCGCCGGC
  GGCGGCAGCAACGGCTCGTCCACCCCCGCGGCGGGGTCGCCTGGGGCCCCCGAGAAGTTGCTGGCTCCGC
  GTCCCGGAGGCTCTCTGCCCGGACGGCTGGAGGGCGGGAGCCCCGCGCGCGGCAGCGCCTTCACCTCGGT
  GTCGCAGCTGGGCGGCGGTGGCGGCGCGGGGACCGCGGGGACTGCGGGGGGCTCGGGGGGCGGCCAAACG
  GCCGCATCGGACGAGCGGAAGAGCGCCTTCTCGCAGCCCGCGCGCTCCTTCTCGCAGCTGTCCCCGTTGG
  TCCTCGGCCAGAAGCTGGGCGCTCTCGAGCCGTGTCACCCGGGAGACGGCGTGGGTCCCACCAGACTCTA
  CCCGGCCGCCGCCGATCCGCTGGCTGTGAAGCTGCAGGGGGCAGCGGACCTGAACGGAGCCTGCGGGCCC
  CTGGCGAGCGGCGGTGGCGGTGGCTTGCCCAAGCAGAGCCCCTTCCTCTACGCCACCGCCTTCTGGCCCA
  AGAGCTCGGCTGCGGCGGCAGCGGCGGCGGCGGCCGCCGCCGGGCCCCTGCAGCTGCAACTGCCCTCCGC
  GCTCACCCTGCTGCCGCCCTCCTTCACTTCGCTGTGTCTGCCCGCGCAGAACTGGTGCGCCAAATGCAAC
  GCCTCCTTCCGCATGACCTCCGACCTGGTGTACCATATGCGGTCTCACCACAAAAAGGAGTATGCCATGG
  AGCCCCTGGTGAAGCGGCGGCGGGAGGAGAAACTCAAGTGCCCCATTTGCAACGAGTCCTTCAGGGAGCG
  TCACCACCTGTCCCGGCACATGACCTCGCATAATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029947
• Insert Size: 2067 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029947.2, NP_084223.2
• RefSeq Size: 3119 bp
• RefSeq ORF: 2067 bp
• Locus ID: 77630
• UniProt ID: Q8BZ97
• Cytogenetics: 5 E3
• Gene Summary: Probable histone methyltransferase, preferentially acting on 'Lys-9' of histone H3 (PubMed:19646955). Histone methyltransferase activity has not been confirmed in other species. Involved in the control of steroidogenesis through transcriptional repression of steroidogenesis marker genes such as CYP17A1 and LHCGR (PubMed:19646955). Forms with BHLHE22 a transcriptional repressor complex controlling genes involved in neural development and neuronal differentiation (PubMed:22284184). In the retina, it is required for rod bipolar and type 2 OFF-cone bipolar cell survival (PubMed:26023183).[UniProtKB/Swiss-Prot Function]