Ccdc8 (NM_001101535) Mouse Untagged Clone

CAT#: MC220481

Ccdc8 (untagged) - Mouse coiled-coil domain containing 8 (Ccdc8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ccdc8
• Synonyms: ENSMUSG00000041117
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220481 representing NM_001101535
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCAGATTGGGGAGGATGTTGACTATCTGCTCATCCCCCGGGAGGTCCGCCTGGCTGGGGGCGTGT
  GGAGAGTCATCTCCAAGCCTGCAACGAAGGAAGCTGAGTTTCGGGAGCGGCTAATCCAGTTTTTGCAGGA
  GGAGGGCCGCACCCTGGAAGATGTGGCCCGTATCATCGAAAAGAGCACTCCACACCCACCCCAACCCCGC
  AAAAGAACCAAGGAGCTGAGAGTGAGGAGAGTCCCGCAGATGGTCACTCCTCCACTTCGGCTGGTTGTAG
  GTACCTATGACAGCAGCAATGGCAGCGACAGTGAGTTGAGTGACTTCGATACCTCCAAAGTCAAAGGCAA
  CAGGAGCTCCTCCGGGAGGACCAGGAAGGTGCGCAAAATGCCGGTCAGTTACTTGGGCAGCAAATTCCTG
  GGCAGCGATGTGGAGAGTGAGGATGATCAGGAGCTGGTGGAGGCCTTCCTCCGCCGTGGGGAGAAGCCCA
  GCGCGCCTCCTCCACGACGCCGAGTCAATCTTCCAGTGCCCATGTTTGAGAACAACCTGGGGCCCCAGCC
  ATCCAAGGCCGACAGATGGAGGGAGTATGTTAGCCAGGTGTCCTGGGGGAAGCTGAAACAGAGGGTGAAG
  GGCTGGGCACCCAGATCAGGCTCCGAGGTGGGCCAGACCCAGCAGGCCTCCACAGCAGCTGAGAGGGCTG
  GAGAAATGAGACACAGCCAAGCCAGCTCGGACGATGATAGCTCCAGAAACACAGGAGACCGGAGTGACCA
  GATGCTGGGTACTAGACGCTGGAAGCCCAAGATCAAGTGGGTTTCCCTGAGACGATGCAGGAAGGAGCAG
  GTGCCACCCTTCGCTCAGGGAACAGGCATGCCAGCTGAGGAGCACCCAGAGGCTGCAGAGAACCAGGGGG
  CAGAGGCTGCAGCTAACCAGAGGGCAGAGCCTCTAGCTAGTCCAAGGGCAGAGGCTGCAGCTAGTCCAAG
  GGCAGAGACTGCAGCTGATCCAAGAGTAGAGGCTGTTGCTAGTCCAAGAGCAGAGGCTGCAGCTAGTCCA
  AGGGCAGAGGCTGTAGCTGATCCAAGAGCAGAGGCTGCAGCTAGTCCAAGGGCAGAGGCTGCAGCTAGTC
  CAAGGACTGAGGCTGCAGCTAGTCTCAGGGCAGAGGCTGTTGCTAGTCCCAGGGCAGAGGCTGCAGCTAG
  TCCAAGGGCAGAGGCTGCAGCTGATCCAAGAGCAGAGGCTGCAGCTAGTCCAATAGCAGAGGCTGCTGCT
  AACCAGAAGGCAGAGCTTGTAGATAGTCCAAGGGCAGAGACTGCAGCTGATCCAAGAGCAGAGGCTGCAG
  CTAGTCCAAGGGCAGAGGCTGTAGCTGATCCAAGAGTAGAGGCTGCAGCTAGTCCAATAGCAGAGGCTGC
  TGCTAACCAGAAGGCAGAGCTTGTAGATAGTCCAAGGGCAGAGACTGCAGCTGATCCAAGAGCAGAGGCT
  GCAGCTAGTCCAAGGGCAGAGGCTGCAGCTGATCCAAGAGCAGAGGTTGCAGCTAGTCCAAGGGCAGAGG
  CTGCAGCTAGTCCAAGGGCAGAGGCTGAAGCTAGTCCAAGGGCAGAGGCTGCAGCTAGTCCAAAGGCAGA
  GGCTGAAGCTAATCTGAGGGTAGAGGCTGCAGCTTATCTCAGGGCAGGAGTCCCACCTGACCAGAGGGCA
  GAAGCCATAGATAGTCAGAGGGCAGAGGGCCCAGCTAACCAAAGAACTGGTGCTACAGAAAATCAGAGGG
  TGGAGGTCCTAGCTGACCAAAGGGCAGGGGTCCTCCATGACCAGAGGGAGGAGGCAGGACCTCAGGGTAT
  ACAGGAAGCCTCAGCTGGTTCGGGATCTAGGGCCCAGAAACAGGTAAAGACAGTGAGGTTCCAGACCCCA
  GGACGCTTCTCATGGTTTCGCATGCGCAGGAGAGTCTTCTGGCACACTCCCAGGTTGCCAACCCTGCCCA
  GGAGAGTCCCCAGGGCAGGTGAGGCCAGGAGCCTCCGGGTACTAAGGGCTGACATCAGAGCAGATGTGGA
  ACACAGAGAGCAAGAAGAACAGCTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001101535
• Insert Size: 2058 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001101535.1, NP_001095005.1
• RefSeq Size: 2058 bp
• RefSeq ORF: 2058 bp
• Locus ID: 434130
• UniProt ID: D3YZV8
• Cytogenetics: 7 A2
• Gene Summary: Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer. Required for localization of CUL7 to the centrosome.[UniProtKB/Swiss-Prot Function]