Thoc5 (NM_172438) Mouse Untagged Clone

CAT#: MC220451

Thoc5 (untagged) - Mouse THO complex 5 (Thoc5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Thoc5
• Synonyms: 1700060C24Rik; A430085L24Rik; Fmip; PK1.3; Sgk2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220451 representing NM_172438
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCATCAGAATCCAGCAAAAAACGGAAACCCAAAGTGATCCGGAGTGATGGAACCCCAACTGAAGGGA
  AACGGAACCGGTCTGACACGGAGCAGGAAGGCAAATACTACAGTGAGGAGGCTGAAGTTGACCTGCGGGA
  CCCTGGCAGAGACTATGAGTTGTACAAATACACTTGCCAGGAGCTACAAAGGCTGATGGCCGAGATCCAG
  GACCTGAAGAGTAAGGGCAGCAAGGACCTGGCCATTGAAATTGAAGAACGAAGGATCCAGAGCTGTGTGC
  ACTTCATGACTCTAAAGAAGCTTAACCGATTAGCCCACATCAGATTAAAGAAAGGAAGAGACCAGACCCA
  CGAGGCCAAGCAGAAAGTCGATGCCTACCACCTACAGCTACAGAACCTGCTCTACGAAGTGATGCACCTG
  CAGAAGGAGATCACCAAATGTCTGGAGTTCAAGTCAAAGCATGAAGAAATTGATCTGGTCAGCTTAGAGG
  AATTTTATTCAGAGGCTCCACCAAGCATCAGCAAGGCCGAGATCACCATGGGAGACCCTCACCAACAAAC
  ATTGGCACGTTTGGACTGGGAGCTGGAGCAGCGGAAAAGGCTGGCAGAAAAGTACCGAGAATGTCTGTCT
  AACAAGGAGAAGATCCTGAAGGAGATTGAGGTGAAGAGGGACTATCTGAGCAGCCTCCAGCCTCGCCTCA
  ACAGCATCATGCAGGCTTCCCTGCCAGTGCAAGAATACCTGTTCATGCCATTTGACCAGGCGCACAAACA
  GTATGAGACTGCCAGGCACCTGCCGCCTCCCCTCTATGTGCTCTTTGTGCAGGCCACTGCATACGGGCAG
  GCCTGTGATAAGACGCTGTCCGTGGCAATTGAAGGCAGTGTGGACGAAGCCAAAGCTCTTTTCAAGCCCC
  CTGAGGACTCCCAAGATGATGAGAGCGACTCAGATGCTGAAGAGGAGCAGACGACGAAGCGGCGGCGGCC
  CACTCTCGGGGTTCAGTTGGATGACAAGCGCAAGGAGATGCTAAAAAGGCACCCACTGTCTGTCCTGCTG
  GATCTGAAGTGCAAAGACAACAGTGTGCTTCATCTGACTTTCTACTATCTCATGAACCTCAACATCATGA
  CAGTGAAAGCCAAAGTGACGACCGCCGTGGAGCTGATCACCCCCATCAGTGCAGGAGACTTACTGTCTCC
  TGACTCGGTCTTGAGCTGCTTGTATCCGGGAGATCATGGGAAAAAAACTCCAAATCCAGCCAATCAGTAT
  CAGTTTGACAAAGTTGGCATCCTGACTTTGAGAGACTATGTACTTGAGTTAGGTCACCCCTACTTGTGGG
  TACAGAAGCTGGGTGGCCTCCACTTCCCCAAAGAGCAGCCCCAGCAAACAGTGATGCCTGACCACTCACA
  GAGCGCCAGCCACATGGAGACCACCATGAAACTGCTGAAGACCAGGGTACAGTCCCGCCTGGCTCTCCAC
  AAACAGTTTGCATCTCTGGAACATGGCATTGTGCCCGTCACCAGCGATTGCCAGGACCTCTTCCCTGCCA
  AGGTTGTTTCTCGCCTGGTAAAGTGGGTGATAATTACCCATGAAGATTACATGGAGCTGCACTTCACTAA
  GGACATTGTGGAGGCAGGACTGGCCGGAGACACCAATCTCTACTACCTGGCGCTAATTGAAAGGGGAACA
  GCTAAACTTCAGGCTGCTGTGGTACTAAACCCTGGCTACTCCTCCATCCCACCGGTTTTCCGACTCTGTC
  TAAACTGGAAAGGGGAGAAAACCAACAGCAACGATGACAATATTCGGGCTATGGAGAGTGAAGTCAACGT
  GTGCTATAAGGAGTTGTGTGGCCCTCGGCCCAGTCACCAGCTCCTGACCAACCAGCTGCAGAGACTGTGT
  GTGCTGCTGGACGTCTACCTGGAGACCGAGAGCCACGATGACAGCTTCGAGGGCCCCAAGGAGTTTCCCC
  AGGAGAAGATGTGCCTGCGGCTTTTTAGGGGTCCGAGTAGGATGAAGCCGTTTAAATACAACCATCCCCA
  AGGGTTCTTCAGCCACCGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172438
• Insert Size: 2052 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172438.3, NP_766026.1
• RefSeq Size: 2235 bp
• RefSeq ORF: 2052 bp
• Locus ID: 107829
• UniProt ID: Q8BKT7
• Cytogenetics: 11 A1
• Gene Summary: Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. THOC5 in conjunction with ALYREF/THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in transcription elongation and genome stability. Involved in alternative polyadenylation site choice by recruiting CPSF6 to 5' region of target genes; probably mediates association of the TREX and CFIm complexes.[UniProtKB/Swiss-Prot Function]