Raph1 (NM_001045513) Mouse Untagged Clone

CAT#: MC220447

Raph1 (untagged) - Mouse Ras association (RalGDS/AF-6) and pleckstrin homology domains 1 (Raph1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Raph1
• Synonyms: 9430025M21Rik; C730009O10Rik; Lpd
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220447 representing NM_001045513
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAACTATCCGATGAAGAGATTGACCATGGTGCTGAAGAAGACAGCGACAAGGAAGATCAGGACC
  TGGATAAAATGTTTGGGGCCTGGCTTGGAGAACTGGACAGGCTTACTCAGAGTTTGGATTCTGACAAGCC
  CAAAGAACCAGTAAAAAGATCTCCTCTTCGCCAGGAAACAAATATGGCCAACTTTTCTTACCGCTTCTCC
  ATATACAACTTGAATGAAGCTCTGAATCAGGGTGAGACTGTGGACCTGGATGCCCTGATGGCTGACCTCT
  GCTCTATAGAGCAGGAGCTCAGCAGCATTGGCTCAGGAAATAGTAAGCGTCAAGTCACAGAACCAAAAGT
  TCCTCAGAAGCTACCTGCTGGCCGACATACGTCAAAACATGGCACCCTGAGAGGACCGTCATCTTCCTCT
  AATAGAACAACTAAACCTTCCCATGCTAACTACTCCCTGGATGACATCACTGCTCAGTTAGAACAGGCTT
  CCTTGAGCATGGATGAGGCTGCTCAGCAATCTCTGGTAGAAGATCCTAAGCCTTTAGTGACAAATCAGCA
  CAGAAGGACTGCATCAGCAGGCACTGTGAGTGATGCTGAAGCGCGCTCCATCAGTAACTCCTCCCGGTCA
  AGCATCACCTCTGCAGCCTCCAGCATGGATTCTCTGGATATTGATAAAGTGACACGCCCTCAAGAGCTTG
  ATTTGACCACACACCAGGGGCAGCCAATTACTGAGGAAGAACAGGCAGCAAAACTGAAAGCTGAGAAGAT
  CCGAGTTGCTCTGGAGAAAATTAAAGAGGCTCAAGTGAAAAAGCTGGTAATCAGGGTCCACATGTCTGAT
  GACAGTTCTAAGACAATGATGGTGGATGAGAGACAGACAGTGAGACAAGTGCTGGATAACCTGATGGACA
  AATCTCACTGTGGCTACAGTTTAGACTGGTCGCTGGTGGAAACCATTTCCGAGTTACAAATGGAAAGAAT
  CTTTGAAGACCATGAAAACTTGGTTGAAAATCTTCTTAATTGGACAAGAGACAGCCAAAATAAGCTTATA
  TTTATGGAGCGTATAGAAAAATATGCACTTTTTAAAAACCCCCAGAATTATCTTCTAGGGAAAAAGGAAA
  CAGCTGAAATGGCAGACAGAAACAAAGAAGTGCTGTTAGAGGAATGTTTTTGTGGAAGTTCTGTAACTGT
  CCCAGAAATTGAAGGAGTCCTTTGGTTAAAAGATGATGGCAAGAAGTCCTGGAAAAAGCGTTATTTTCTC
  TTGCGAGCATCTGGTATCTACTATGTCCCCAAAGGAAAAGCAAAGGTATCTCGGGATCTAGTGTGCTTTC
  TCCAGCTGGATCATGTAAATGTATACTATGGACAGGACTATCGGAACAAATACAAAGCACCCACAGACTG
  CTGCCTGGCATTAAAGCACCCACAGATCCAGAAGAAGTCTCAGTATATCAAGTACCTTTGCTGTGATGAT
  GCAAGGACCCTGCATCAGTGGGTCAATGGAATCCGCATTGCCAAGTATGGGAAGCAGCTCTACACGAATT
  ACCAAGAAGCCTTGAAGAGGACAGAGTCCGCTTATGATTGGACTTCGTTATCCAGCTCCAGCATTAAATC
  CGGATCCAGTTCTTCCAGTATCCCGGAGTCTCAGTCAAACCACTCTAATCAGTCTGACAGCGGAGTGTCT
  GACACCCTGCCAACTGGACATATCCGTTCCCAAAGTACCGTGAGCTCCATCTTCTCGGAAGCCTGGAAGC
  GAGGCACTCAGCTGGAAGAGTCCAGCAAGGCCAGAATGGAGTCTATGAATCGGTCCTACACTTCACTTAT
  GCCCCCTCTGTCCCCTCAAACTAAGATCATCACCCCCTATACTGCCTCACAACCTTCACCGCCTCTTCCT
  CCTCCTCCGCCACCACCTCCTCCTCCTCCACCTCCGCCACCACCACCTCCTCCTCCTCTTCCCAGCCAGT
  CTGCACCATCTTCAGGCTCAGCAGCTACCATGTTTGTCAAGTACAGCACAATCACCAGGCTGCAAAATGC
  AGCTCAGCATTCAGGGACCCTGTTTAAGTCTCCAACACCACCAGCGATGCAGCCTCTTCCTTTGGCTTCA
  CAGTCCAAGGCTCAAATTGTGGTGCCCCCCAATGGAGTTATTCCTCCACCCCCTCCTCCGCCACCACCCC
  CAACCCCTGGCTCAGCCATGGCCCAGTTAAAGCCAGTGCCATGTGCCCCATCCCTACCACAGTTCAGCCC
  CCAACCTCCTCCACTGAAGATTCACCAGGTTCAGCACCTTCCTCAGACAGCCCCTCCTACACCCCCACCA
  GCTCCTCCCATCACTGCAACTGTCCCTCCTCAAGCACCCCCTAAACCTCTGGTGACCATCCCTCCTTCAG
  CCAGCACAAAGACTGTGTCACCAGTAGTAGCACAAGCTGTACCACCAACCCCTGCACCTCCTGTGCCCCC
  TGCAAAGAAACAGCCAGTTTTCCCTGTTTCTCACATTCCACCCACTCCCCCTGTGCAGCCTACCCCACAT
  CCCCCCCCTACACTACCCAAACAGCAGAGCTTTGGGGTAAAGCCACCCCCCTCTCCACTGTCCCCTGTGC
  CCTCCGTTGTGAAGCAGATAGCCAGTCAGTTTCCACCCCCTCCCACTCCTCCTCCTGTGGACTCTCAGCC
  CCTAAAGTCCCTGCCAGCAAGTGGCACCCCACAGTCCCCTCCTGCAGTAAAAGCAAAACCCAAATGGCAG
  CCCAGCTCAATCCCTGTCCCTTCTCCAGATTTCCCTCCTCCCCCTCCTGAAAGCAGCCTGGTGTTTCCTC
  CACCACCTCCTCCAGCCCCAGCCCCAGCCCCACCACCACCACCACCACCACCTCCACCACCGCCACCAGT
  GCCACCCACGGCTTCACCTACCCCTGACAAAGGTGGATCTCCAGGCAAAAAGACAAGTAAGATGTCCAGC
  CCTGGGGGAAAGAAACCACCCCCAACCCCGCAGCGCAACTCCAGCATTAAGTCCGGCAGTTGTGCAGAGC
  ACCCTGAGCCCAAGCGACCCTCGGTAGACAGTCTAGTCAGCAAGTTTGCACCGCCAGCAGAATCAGGGTC
  TCCCAGCAAAGAGACCCCACCACCTCCTGCAGCACCCCCCAAACCTGGGAAACTACACCTTTCTGGAGTC
  AATCTCCCTGGAATCCACCAGCAAGGGATTGTGTCGGCAAAAGCCTCTGTTCTGAGTGGGCGTGGAAAGG
  ACGTTGTAGTGGAATTTCCATCGCCACCATCTGATTCTGACTTTCCACCCCCTCCACCTGAAATAGAGCT
  TCCTCTGCCCCCTATTGAGATTCCAGCTGTATTCTCGGGGAACACCTCCCCCAAAGTGGCAGTTGTTAAT
  CCTCAGCCACAGCTATGGTCTAAAACATCAGTGAAGAAGGCCCCCCCACCTACCCGGCCCAAACGCAATG
  ACAGCACCCGCCTCACTCAAGCTGACATATCTGAACAGCCAGCAATGACCACAGTTGTGCCACAAGTGCC
  CACCTCTCCTAAATCCAGCCTTAGCGTCCAACCTGGATTCCTAGCTGATCTTAACAGGACATTGCAGCGC
  AAGTCTATCACGCGGCATGGCTCCCTGTCCTCCTCACGTATGTCCAGAGCAGAACCCACCGCCACAATGG
  ACGACATGGCACTACCCCCACCACCACCAGAACTGCTGTCTGACCAGCAGAAGGCTGGCTTTGGAGGTAG
  TCATATTTCGGGCTATGCAACATTGCGAAGAGGACCCCCTCCCGCTCCTCCTAAGAGAGACCAGAATACT
  AAGCTCTCAAGAGACTGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001045513
• Insert Size: 3801 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001045513.3, NP_001038978.3
• RefSeq Size: 9117 bp
• RefSeq ORF: 3801 bp
• Locus ID: 77300
• Cytogenetics: 1 C2