Mid2 (NM_011845) Mouse Untagged Clone

CAT#: MC220427

Mid2 (untagged) - Mouse midline 2 (Mid2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mid2
• Synonyms: FXY2; Trim1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220427 representing NM_011845
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACACTGGAGTCAGAATTGACCTGTCCAATCTGCCTCGAGTTGTTTGAAGACCCCCTTCTGCTTC
  CTTGTGCTCACAGCCTATGTTTTAGCTGTGCCCACCGCATCTTGGTCTCAAGCTGCAGCTCTGGTGAATC
  CATTGAACCCATTACTGCTTTCCAGTGTCCTACATGCAGGTATGTTATCTCGCTGAATCACCGGGGCCTG
  GATGGCCTCAAGAGGAATGTGACCCTGCAGAACATTATTGATCGCTTCCAGAAGGCTTCAGTCAGTGGGC
  CCAATTCTCCAAGTGAGAGCCGCCGGGAGAGGACTTACAGGCCTAGCTCCGCCATGTCGAGTGAGAGAAT
  TGCATGTCAATTCTGTGAGCAGGACCCTCCGAGAGATGCTGTAAAGACGTGCATCACCTGTGAGGTCTCC
  TACTGTGACCGTTGCCTTCGGGCCACACACCCCAACAAGAAACCTTTCACCAGCCATCGCCTGGTGGAAC
  CAGTTTCAGACACACATCTTCGAGGGATTACCTGCCTGGACCACGAGAATGAGAAGGTGAACATGTACTG
  TGTATCTGATGATCAATTGATCTGTGCCTTATGCAAACTGGTGGGTCGTCACCGAGACCATCAGGTCGCT
  TCTCTGAATGATCGATTTGAGAAACTAAAGCAAACTCTCGAGATGAACCTCACCAACCTGGTTAAGCGCA
  ACAGTGAACTAGAAAATCAAATGGCCAAACTAATACAGATCTGCCAGCAAGTTGAGGTGAATACTGCTAT
  GCATGAGGCAAAACTTATGGAAGAATGTGACGAGTTGGTAGAGATCATCCAGCAGAGGAAACAAATGATT
  GCTGTCAAAATCAAAGAGACAAAGGTTATGAAACTGAGAAAGTTGGCACAGCAGGTTGCTAATTGCCGCC
  AGTGTCTTGAGCGGTCAACAGTCCTCATCAACCAAGCTGAGCATATCCTAAAGGAAAATGACCAAGCACG
  GTTTCTGCAGTCTGCAAAAAACATTGCCGAGAGGGTCGCTATGGCAACTGCATCTTCGCAAGTTCTGATT
  CCAGACATCAATTTTAATGATGCCTTTGAAAACTTTGCTTTAGATTTTTCTAGAGAAAAGAAACTATTGG
  AGGGGCTAGACTATTTAACAGCTCCAAACCCACCATCTATCCGAGAAGAGCTCTGTACTGCTTCCCATGA
  CACCATTACAGTCCACTGGATCTCAGACGACGAATTCAGCATCAGCTCCTATGAGCTTCAGTACACCATA
  TTCACTGGCCAGGCTAACTTCATCAGCCTGTACAATTCAGTGGATAGCTGGATGATTGTGCCCAACATTA
  AGCAGAACCATTACACAGTTCATGGACTCCAGAGTGGGACACGCTACATCTTTATCGTGAAAGCCATAAA
  TCAAGCAGGCAGCCGGAACAGCGAACCCACCAGACTAAAAACGAACAGCCAACCATTTAAACTAGATCCC
  AAAATGACTCACAAGAAGTTGAAGATCTCCAATGATGGATTGCAGATGGAGAAGGATGAGAGCTCTCTGA
  AGAAGAGCCACACTCCGGAGAGATTTAGTGGCACAGGGTGTTATGGGGCAGCAGGAAATATATTCATCGA
  CAGTGGCTGCCACTACTGGGAGGTGGTCATGGGTTCTTCAACATGGTATGCTATTGGTATTGCCTACAAA
  TCAGCTCCAAAGAATGAGTGGATTGGCAAGAATGCCTCTTCCTGGGTCTTCTCTCGATGCAACAGTAACT
  TCGTGGTTCGGCACAACAACAAGGAAATGCTGGTGGATGTGCCCCCACAGTTGAAGCGTCTAGGTGTCCT
  TCTGGATTATGACAACAACATGCTGTCTTTCTATGACCCAGCTAACTCTCTCCATCTTCATACTTTTGAT
  GTTACCTTCATTCTTCCAGTTTGTCCAACATTCACAATCTGGAACAAATCCCTAATGATTCTTTCTGGCT
  TGCCTGCCCCTGATTTTATTGATTATCCTGAGCGGCAGGAATGCAACTGCAGGCCTCAAGAATCCCCTTA
  TGTGTCAGGGATGAAAGCTTGCCATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011845
• Insert Size: 2058 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011845.2, NP_035975.1
• RefSeq Size: 5916 bp
• RefSeq ORF: 2058 bp
• Locus ID: 23947
• UniProt ID: Q9QUS6
• Cytogenetics: X 61.35 cM
• Gene Summary: May play a role in microtubule stabilization.[UniProtKB/Swiss-Prot Function]