Gtf3c4 (NM_001166033) Mouse Untagged Clone

CAT#: MC220384

Gtf3c4 (untagged) - Mouse general transcription factor IIIC, polypeptide 4 (Gtf3c4), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gtf3c4
• Synonyms: 5330400C03; AI426938; AU014771; AU017413; KAT12; TFIIIC90
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220384 representing NM_001166033
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTAGACAGGATGTTCAACCCCGAGGGGAAGGCCTTGCCCCCAATGCGGGGTTTCAAATACACTAGCT
  GGTCTCCAATGGGTTGTGATGCAAATGGCAGGTGCCTCTTGGCAGCACTGACCATGGACAATCGCCTGAC
  TGTGCAGGTGAACCTCAACAGACTCCAGTGGGTTCAGCTGGTTGATCTGACGGAGATTTATGGAGACCGT
  CTTTATGAGACTAGTTATAGACTGTCTAAAAATGAGGCTCCTGAGGGAAATCTCGGGGATTTTGCTGAGT
  TTCAGAGGAGACACAGTATGCAGACCCCAGTCAGAATGGAATGGTCGAGTATCTGTACCACTCAGCAGGT
  CAAGCACAACAACGAGTGCCGGGATGTGAGCAGCGTGCTGCTGGCTGTCCTCTTTGAGAACGGGAATATA
  GCTGTGTGGCAGTTCCAGCTGCCATTCGTGGGGAAGGAGTCCATCTCTTCATGCAACACTATTGAGTCAG
  GAATCAGCTCCCCCAGTGTTTTGTTTTGGTGGGAATATGAGCACAATAATAGGAAAATGAGTGGCCTTAT
  TGTGGGCAGTGCTTTTGGACCTGTAAAAATTCTCCCTGTCAATCTCAAAGCAGTGAAAGGCTACTTTACT
  TTAAGGCAGCCTGTTGTCTTGTGGAAAGAAATGGACAAGTTACCTGTGCACAGCATCAAATGTGTGCCAC
  TTTACCACCCCTACCAGAAGTGTAGCTGTAGCTTAGTGGTAGCTGCAAGAGGCTCCTATGTGTTTTGGTG
  TCTTCTTCTGATCTCCAAGGCAGGTCTGAATGTTCACAATTCCCACGTCACAGGCCTCCACTCACTGCCC
  ATCGTCTCCATTACTGCAGACAAGCAGAATGGAACAGTGTACACCTGCTCTAGTGATGGGAAGGTGAGGC
  AGTTGATTCCCATTTTCACAGATGTTGCATTAAAGTTTGAACACCAGCTGATTAAGCTCTCAGATGTCTT
  TGGCTCAGTGAGGACTCACGGCATTGCAGTGAGTCCCTGTGGTGCTTATCTGGCCATCATCACCACTGAG
  GGCATGATGAATGGCCTCCATCCTGTGAATAAGAACTACCAGGTCCAGTTTGTTACTCTGAAGACCTTTG
  AAGAGGCAGCTGCTCAGCTCTTGGAGTCTTCAGTTCAGAATCTCTTTAAGCAGGTAGACTTGATCGACCT
  GGTCCGCTGGAAAATTTTAAAAGATAAACATATTCCTCAGTTTTTACATGAAGCATTGGAAAAAAAGATT
  GAAAGCAGCGGGGTTACCTATTTTTGGCGTTTTAAGCTTTTCCTTCTAAGGATTTTGTATCAGTCAATGC
  AGAAATCCCCTTCCGAGGCCTTATGGAAACCCACCCATGAGGACTCAAAAATCTTACTGGTTGACTCACC
  TGGGATGGGCGACGGTGAGGATGAGCAGCAAGAAGAAGGTACTTCCAAGCAGGGGACTAAGGCTGGCCTG
  CAGGAGAAGAGCAAAGAAGGTGACACAGAGGAGACCCCTGAGGACTCACTCACTGCAGGGGGAGACACCG
  GAGGCCGTGAACCTGTAGAGGAAAAGCTCCTTGAGATCCAAGGGAAGATTGAGGCTGTGGAGATGCACCT
  GACCAGGGAGCACATGAAGCGGGTCCTGGGAGAAGTGTACCTGCACACCTGGATCACAGAGAACACCAGC
  ATACCCACCAGAGGGCTCTGTAACTTCCTGATGTCTGATGAGGATTATGATGACAGGACAGCACAGGTCT
  TGATTGGACATATCTCCAAGAAGATGAACAAACAGACCTTCCCTGAGCGCTGCAGTCTGTGTAAGGAGAT
  CTTACCATTCACAGATCGCAAGCAAGCTGTTTGTTCCAATGGGCACATCTGGCTCCGGTGCTTTCTAACC
  TACCAGTCCTGCCAGAGCTTGATATACAGACGGTGTTTGCTCCATGACAGCATCGCCAGGCATCCTGTTC
  CAGAAGATCCTGACTGGATTAAGAGGTTGCTGCAGAGTCCTTGTCCTTTCTGTGATTCTCCTGTCTTCTG
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001166033
• Insert Size: 2031 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166033.1, NP_001159505.1
• RefSeq Size: 7127 bp
• RefSeq ORF: 2031 bp
• Locus ID: 269252
• UniProt ID: Q8BMQ2
• Cytogenetics: 2 A3
• Gene Summary: Essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate exon compared to variant 1, that causes a frameshift and a downstream translation start site to be used. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.