Fermt1 (NM_198029) Mouse Untagged Clone

CAT#: MC220378

Fermt1 (untagged) - Mouse fermitin family homolog 1 (Drosophila) (Fermt1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fermt1
• Synonyms: 5830467P10Rik; Kindlin-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220378 representing NM_198029
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGTCATCTGGTGACTTGACATCTGCATCCTGGGAGCTTGTGGTCCGAGTTGACCATGCAAATGGAG
  AGCAGCAGACAGAGATCACGCTGAGGGTGTCTGGGGACCTACATATCGGGGGAGTGATGCTCAAGCTCGT
  GGAACAGATGAACATAGCCCAAGACTGGTCAGACTATGCTCTGTGGTGGGAACAGAAGCGCTGCTGGCTT
  CTGAAAACACACTGGACCCTGGACAAGTGTGGGGTCCAGGCTGACGCCAACCTTCTCTTCACCCCTCAGC
  ATAAGATGCTTCGCCTTCGCCTGCCCAATGCCAAGACCGTGCGCCTGCGCGTCAGCTTTTCGGCTGTGGT
  GTTTAAGGCTGTCGCTGATATCTGCAAAGTGCTGAATATCAGAAGACCAGAAGAACTTTCATTGTTAAAG
  CCTTCTAGTGACTACTGTAAAAAGAAAAAAAAGAAAGAAAAAAATAGTAAAGAGCCAGTGATTGAAGACA
  TCCTGAACCTGGAAAGTTCTTCAACAAGTTCAGGTTCCCCAGTAAGCCCCGGCTTGTACAGTAAGACCAT
  GACACCCACATACGACCCCATCAATGGGACACCAGCCTTATCCACTATGACCTGGTTTGGTGATAGCCCT
  TTGACAGAGCAAAACTGCAGTGTTCTGGCCTTCAGCCAACCTCCTCCATCACCAGATGTCCTTGCCGACA
  TGTTCCAGCCTCGATCTTTGGTTGACAAAGCCAAGATGAATGCAGGCTGGCTGGATTCCTCACGATCCCT
  TATGGAGCAAAGCATTCAGGAGGATGAGCAGCTGCAGTTGAGATTTAAATACTACACCTTCTTTGACTTG
  AATCCTAAATATGACGCTGTCAGAATAAACCAACTCTACGAGCAAGCCAGGTGGGCGGTTCTCTTGGAGG
  AAATCGACTGCACAGAAGAAGAAATGTTGATCTTTGCAGCCCTGCAGTATCACATCAGCAAGCTGTCCCA
  GTGTGCTGAAATACAGGACTTTGCAACCAAGTCTGAGGTTGACGAGGTAGAAGCAGCATTGTCTAGTTTG
  GAAGTGACCCTGGAAGGCGGGAAGGCCGACAACACTTTGGAGGACATAACTGACATCCCTAAACTCGCAG
  ATTATCTCAAATTATTTAGGCCCAAGAAGCTGATGTTAAAAGCTTGCAAGCAATACTGGTTTGTCTTTAA
  AGACACATCTATAGCCTACTTTAAAAATAAGGAGCTTGAACAAGGAGAGCCAATAGAGAAGCTAAACCTT
  AGAGGCTGCGAAATTGTGCCAGATGTGAATGTATCGGGGAGAAAATTTGGAATCAAGTTACTAATCCCCG
  TTGCTGATGGTATGAATGAAGTGTACTTGAGATGTGACCATGAGGATCAATATGCCCGATGGATGGCTGC
  CTGCATCTTGGCATCGAAGGGTAAAACTATGGCAGACAGCTCCTATCAGCCAGAGGTCATCAGCATCCTT
  TCGTTTCTCAAGATGAAAAACCGGAACTCATCACCTCTGGTGGCTTCCAGTCTTGAAAACATGGATATGA
  ACCCGGAATGTTTAGTATCGCCCTGCTGTGCAAAAAAGCACAAGTCTAAACAGCTGGCGGCCCGGATCCT
  GGAAGCTCACCATAACGTAGCCCAGATGCCTCTGGTCGAAGCCAAACTGCAGTTCATCCAGGCCTGGCAA
  TCCTTACCTGAGTTCGGCCTCACCTACTACCTTGTCAGATTTAAAGGAAGCAAAAAAGATGACATCCTGG
  GAGTTGCATACAACAGGTTGATTAGAATCGATGCGGTCACTGGGATACCAGTTACAACGTGGAGATTCGC
  CAATATGAAGCAGTGGAATGTCAACTGGGAAATCCGGCAGGTGGCAATCGAGTTTGACCAAAACGTCTCC
  ATCGCATTCACCTGCCTAAGTGCAGATTGCAAGATTGTCCATGAGTACATTGGTGGCTACATCTTCTTGT
  CCACCCGATCCAAGGACCAAAACGAAACGCTCGATGAGGACCTCTTCCACAAACTGACTGGCGGTCAGGA
  CTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_198029
• Insert Size: 2034 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198029.2, NP_932146.2
• RefSeq Size: 5255 bp
• RefSeq ORF: 2034 bp
• Locus ID: 241639
• UniProt ID: P59113
• Cytogenetics: 2 F2
• Gene Summary: Involved in cell adhesion. Contributes to integrin activation. When coexpressed with talin, potentiates activation of ITGA2B. Required for normal keratinocyte proliferation. Required for normal polarization of basal keratinocytes in skin, and for normal cell shape. Required for normal adhesion of keratinocytes to fibronectin and laminin, and for normal keratinocyte migration to wound sites (By similarity).[UniProtKB/Swiss-Prot Function]