Ripor2 (NM_001080381) Mouse Untagged Clone

CAT#: MC220363

Fam65b (untagged) - Mouse family with sequence similarity 65, member B (Fam65b), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ripor2
• Synonyms: 1700108N18Rik; 6330500D04Rik; AI225904; E430013J17Rik; Fam65b; RP23-461O24.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220363 representing NM_001080381
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACCCGGTACTAAGCGTCTCCGGGCACTAGGAGCGTTCTCGGCAGGACTGCCAACCAGACTCCCAG
  AAATCATGCTGGTGGGATCCCAGTCCTTCTCTCCTGGCGGGCCCAATGGCATCATCCGGAGCCAGTCCTT
  CGCAGGTTTCAGTGGGCTGCAGGAAAGGCGGTCCAGGTGTAACTCCTTCATTGAAAATGCCTCGGCTCTC
  AAGAAGCCTCAGGCCAAGCTGAAGAAAATGCATAATCTTGGACACAAAAATAACAACACTCCCAAGGAGC
  CTCAGCCGAAGAGAGTGGAGGAGGTCTACCGGGCCTTGAAGAACGGGCTTGATGAGTACCTGGAGTTTCA
  CCAGACGGAGCTGGACAAATTGACTGCTCAATTAAAAGATATGAAGAGAAACTCTCGCCTGGGTGTGCTG
  TATGACCTAGACAAGCAAATTAAAACGATTGAGAGATACATGCGACGCCTGGAGTTTCACATTAGTAAGG
  TAGACGAGCTCTATGAAGCCTATTGTATCCAACGGCGTCTCCAAGATGGTGCCAGCAAAATGAAGCAAGC
  CTTCGCGACGTCCCCTGCCAGCAAGGCTGCCCGGGAGAGTCTGTCTGAGATCAACCGCAGCTACAAGGAA
  TACACGGAGAACATGTGTGCCATTGAAGCGGAGTTGGAGAGTCTGCTGGGAGAATTCTCCATCAAGATGA
  AAGGTCTGGCTGGCTTTGCCCGCCTCTGTCCTGGAGATCAATATGAAATTTTCATGAAGTATGGTCGGCA
  GAGGTGGAAACTGAAGGGCAAAATAGAAGTGAATGGTAAACAGAGCTGGGATGGAGCAGAAACCGTCTTC
  CTGCCCCTCATAGTTGGGTTCATTTCAATCAAGGTCACAGAACTCAAAGGACTGGCGACTCACATCCTGG
  TAGGCAGTGTGACCTGCGAGACCAAAGAGCTGTTTGCAGCCCGACCCCAGGTGGTGGCTGTTGACATCAA
  TGACCTTGGTACCATCAAACTGAACCTGGAAATCACCTGGTATCCCTTTGACGTGGAGGACACAACCCCA
  TCGTCGGGCCCTGGGAACAAGACAGCTGCTCTCCAGCGAAGGATGTCCATGTACAGCCAGGGCACCCCAG
  AAACACCTACCTTCAAAGACCAGTCCTTCTTTAGATGGTTGCGGCTGTCTGTCTTGAGTGCCTTGCGAGA
  CACTTTCTTTGCCACGTTGCACCACAACCACTCTGTCGGTGACCTGCCTTCCCTCAGCCTGAACCCCAAG
  GCCCTGCTAGAATTCTATTCAAATCTGCCAGATGACATCTTTGAGAGTGGAAAGGCTGCAGAGGAGAAAA
  GGCCACTGTCCCTCAGCTTCAGTGACCTGCAGGATGGAGACTGTGTCTTCACCTCCAGCTCAGCCACCTC
  CCCCTCCAGTTCACACTCAGCCCATCCAGAGATTACCATCACCCCCGCAGAGCTTACCCACAGCAGCCTG
  TCCTCACAAAATGAGGGCACCGAGGACAGCAGCTCAGCGTCTTCCAGGAATTCCTTGGGTGAGGACCATG
  AGCCCAAGTCCCACCCAAAGAGTGATACGGTAGAGCCCGGGAAGCCGGGTGTGGCTACAAGGTCTGGTAC
  CGAGAGCCTGTTTCTGGAGAGTAGTGTGGCCGAGGCTCTCCTGCAGGAATCGGATGAGGCCTCTGAGCTC
  AAGCCCGTGGAACTGGACACTTTTGAAGGAAACATCACAAAGCAACTGGTGAAGAGGCTCACCTCGGCTG
  AGGGGCCCGTCACTACTGACAAGCTTTTCTTTGAAGGCTCTGTTGGTAGCGAGTCTGAGGCTGGCCGGTC
  CTTTCTGGATGGCAGCCTGGAAGATGCCTTCAATGGACTCTTCCTTGCATTAGACCCACACAAGAAGCAG
  TACAAAGAGTTCCAGGATCTGAACCAAGAAGTCACTCACTTGGATGATGTTCTCAAAGATGCTAAACATC
  TTGAGGATCAGAGACTCAATGATGCTGCTTCCCGGATGGAGATCACAGAGGGTGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001080381
• Insert Size: 2019 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080381.1, NP_001073850.1
• RefSeq Size: 3434 bp
• RefSeq ORF: 2019 bp
• Locus ID: 193385
• UniProt ID: Q80U16
• Cytogenetics: 13 A3.1
• Gene Summary: Acts as an inhibitor of the small GTPase RHOA and plays several roles in the regulation of myoblast and hair cell differentiation, lymphocyte T proliferation and neutrophil polarization (PubMed:25588844, PubMed:27269051). Plays a role in fetal mononuclear myoblast differentiation by promoting filopodia and myotube formation (PubMed:17150207). Maintains naive T lymphocytes in a quiescent state and prevents chemokine-induced T lymphocyte responses, such as cell adhesion, polarization and migration (By similarity). Involved also in the regulation of neutrophil polarization, chemotaxis and adhesion (PubMed:25588844). Required for normal development of inner and outer hair cell stereocilia within the cochlea of the inner ear (PubMed:27269051). Plays a role for maintaining the structural organization of the basal domain of stereocilia (PubMed:27269051). Involved in mechanosensory hair cell function (PubMed:27269051). Required for normal hearing (PubMed:27269051).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks several exons, and uses an alternate 3'-terminal exon, compared to variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1.