Nol9 (NM_028727) Mouse Untagged Clone

CAT#: MC220353

Nol9 (untagged) - Mouse nucleolar protein 9 (Nol9), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nol9
• Synonyms: 4632412I24Rik; 6030462G04Rik; AI449622; AW490720
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220353 representing NM_028727
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGTCGGAGGTGTTACACCGGCGGGCTCCGAGCCGCTCTTCCTGGCTGCGGGTACGCAAAGCCC
  GGCCCCACCTCCTCCTCAGCCGCCGGGGCCGCCGTCGGTTTGGGGTCCTGACCCGAGTCGAGCTGCGACG
  CCTGCGACGGCGGCTGCTGCGGGCACACGCGTTAGGCGGGGACTGGAAGCAGGTCGCCCCCGCGGGCGCG
  CACGTGGCGGTAAAGTGCAAACTCCGGGCCCGCAGCCGGCCGGCGCCCCGCAGCCCGCCCACACCGAGCG
  TCCCGCCCGCGCCATGCACCGCGTCCGCGACCTGCAGCCTGCTCAACCCCCGCAACCACTCCACGCCCCA
  GAGCCGGGCCGGGCGCCCCGTGCGGAAGGTGTCCCCGAACGTCACCCAGCCCGTGCGGGACCTGGGCTCG
  GGCCGCGTGTTGATGATGCTGCCGCCGGGAGAGGGTTTCACTTTCAGTGGGATCTGCCGTGTGACTTGCG
  TCTACGGCCAGTTGGAAGTATACGGCCATATCATCAACCAAGGGCAGCCTCCCCAAGATGTCTTTTCTGT
  CTACACCCACTCTTACCTGACCATCAATGGGGTTCCGTACGCAGAACCCGAGAAAAGTGAGAAGGCGATC
  AGGAGGGAGATCCGGGCCCTGCTCAAGCCTTACACGAAACTAGATGACAGAAACTGGGTGGTGCGGTACT
  TCCCTCCTCTGGGCTCCATTATGATTCTGGAACGGATGCAGTCTCGTTTTGTGGACTTCCTGAAGACCTA
  TAAGTGCTCCTCATACGTGCTCCTGCAGGAGAACGCTCCTGTTCGGGTCAATTCTGAGTTTACAACTTTG
  AAGAAAATTGGCATCAGAAGACAGAAGAGGAAGAAAGCCATTTGCTTGAGTGAGAGTGGCCTTTGTGCCC
  TGGAAGAGTTAGTCAGCGTGTCCTGTGATGGCTGCCCTGTCATCCTGTTGTGTGGCGCTTGTGACATTGG
  AAAGTCAACGTTCAATAGAATACTGATTAACCAGTTATTAAATAGCATTCCTGGGGTTGACTATCTGGAA
  TGTGATCTGGGGCAGACGGAGTTCACTCCTCCTGGCTGTGTCGCTTTACTTACCATCACAGAACCACTTC
  TGGGACCGCCGTACACTCACCAGAGGAAACCACAGAGGATGGTGTACTATGGGAAGATGAACTGTTACAA
  TGACTACGAGAATTACATTGACATAGTAAAATACGTGTTCAGAGATTACAAGAGAGAGTTTCCACTTATC
  ATCAACACGATGGGCTGGGTGTCAGATAATGGGCTGCGGCTGCTTGTGGACCTGATCCGGGTGCTGTCTC
  CCAATTATGTCGTTCAGTTATATTCTGATCGCTGTAAATTTACACCGACACTGACCTCCGAATACGTAGA
  GCTCACAGATGGCCTGTATACAAAAAGCAAGATCAAAAGATATCGAGGTTTTGAAATTCCAGAATTTGGA
  GACAATTTAGAATTTACTTATGAAGAGAAAGAGTCTAGTCCACTTCCAGTCTTCACTGGACATGTACTGC
  TGTCTGTTCACTCCGAATTCTTAAGTAGTAAAAATGAAAAGAACAGGGCCAAATACAACAGGATTTTTCG
  AGATCTGGCAGTGTTGGGCTACCTTAGCCAGCTGATGCTTCCCGTGCCAGAGTCACTTAGTCCTTTGCAC
  AGCCTGACGCCCTATCAGGTCCCCTTCAGTGCCGTCGCTATCCGGGTCTTGCATGCCGACGTTGCGCCTA
  CCCACATACTGTATGCTGTGAATGCCAGCTGGGTCGGCCTTTGCAGGATCGTGGATGATATGAAAGGCTA
  CACTCGGGGCCCCATCCTGCTTGCCCAGAACCCCATATGTGACTGTTTGGGCTTTGGTATCTGCAGAGGC
  ATTGACATGGACAAGCGGACCTACCACATCCTCACCCCTCTACCCCCAGAAGAGTTAAAAACTGTGAACT
  GTCTGCTGGTTGGTTCCATTTCCATCCCACATTGTATTTTTCAGAACCAGCTTTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_028727
• Insert Size: 2016 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028727.3, NP_083003.2
• RefSeq Size: 2297 bp
• RefSeq ORF: 2016 bp
• Locus ID: 74035
• UniProt ID: Q3TZX8
• Cytogenetics: 4 E2
• Gene Summary: Polynucleotide 5'-kinase involved in rRNA processing. The kinase activity is required for the processing of the 32S precursor into 5.8S and 28S rRNAs, more specifically for the generation of the major 5.8S(S) form. In vitro, has both DNA and RNA 5'-kinase activities. Probably binds RNA (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate 3' terminal exon, and as a result, lacks a portion of the 3' coding region and has a distinct 3' UTR compared to variant 1. It encodes isoform (2), which has a shorter C-terminus than isoform 1.