Zbtb33 (NM_020256) Mouse Untagged Clone

CAT#: MC220344

Zbtb33 (untagged) - Mouse zinc finger and BTB domain containing 33 (Zbtb33), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zbtb33
• Synonyms: AW260253; E130014G12Rik; Kaiso; Znf-kaiso
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220344 representing NM_020256
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGTAGAAAACTGATTTCTGCTACAGACATTCAGTACTCTGCCAGTCTGCTGAACTCCTTGAATG
  AACAGCGTGGCCATGGTCTCTTTTGTGATGTTACTGTTATTGTGGAAGACCGAAAATTCCGGGCCCATAG
  GAACATCCTTTCAGCCTCGAGTACATACTTCCATCAGCTCTTCTCAGTTGCTGGGCAAGTTGTTGAACTA
  AGCTTTATAAGAGCTGAGATTTTTGCAGAAATTCTGAACTATATCTACAGTTCCAAAGTTGTCCGTGTTA
  GAGCAGATTTGCTTGACGAGTTAATTAAATCAGGGCAGTTACTAGGCGTGAAATTTATAGCAGAGCTTGG
  TGTCCCACTGTCACAGGTTAAAAGCATATCAGGTACAGAACAGGATGGTACTGCTGAGACCTTACCTTCT
  AGTTCTAGTGACAAAAGCCTTGACATGGAAAAATCAAAAGATGAAGCCCAAGATAATGGGGCTACAGTAA
  TGCCCATTATAACTGAGTCTTTTTCACTATCTGCTGAAGATAATGAAATGAAAAAGATCATTGTTACTGA
  CTCAGATGATGATGATGATGACGATGTCATTTTCTGCTCTGAAATTTTGCCTGCAAAGGAGGATTTGCCA
  AGTAACAACACAGCAACACAGGTCCAGCCTAACCCAGCCTCTGTTGCTATTTCGGAAGTGACACCTTGCG
  CTAGCAATAACTCGCCCCCTGTAACAAACATCACACCTACTCAACTTCCCACTCCTGTGAATCAGGCAAC
  TCTAAGCCAAACACAAGGAAGTGAAGAATTGCTAGTGTCTTCAGCTTCAACACATCTGACTCCTAACATT
  ATTTTGTTAAATCAGGCTCCACTTACTGCACCACCGAGTGCCAGTTCTTCACTCCCAAATCATATGTCCT
  CTTCAGTCAATGTCCTTGTACAGAATCAGCAGACGCCTAACAGTGCTGTTTTAACAGGAAACAAGGCTGA
  GGAAGAGGAAGAAATTATAGATGATGATGATGACATCATTAGCTCCAGTCCAGACTCAGCAGTCAGTAAT
  ACATCTCTGGTCCCTCAGGCTGATAACTCCAAAAGTACCACTTTAGATGGATCATTGACACAGAAGATGC
  AGATTCCTGTACTTCCTCAAGAGCCACCTTCTAATTCTTTAAAAATTTCAGATGTCATTACTAGAAACAC
  TAATGATCCAGGCTTAAGGTCAAAACATGTAATGGAGGGTCAGAAGATCATTACATTAGATACAGCTACT
  GAAATCGAAGGCTTGTCAACTGGTTGCAAGGTTTATGCAAATATCGGTGAAGATACTTACGATATAGTGA
  TCCCTGTCAAAGATGACCCTGATGGAGGGGAGGCCAAACTTGATAATGAGCTACCAAAAACATCTGGCAG
  TGAGCCACCAAACAAGCGTATGAAAGTAAAACATGATGATCACTATGAGTTAATAGTAGATGGAAGGGTC
  TATTATATTTGCATTGTATGCAAAAGGTCCTATGTCTGTCTGACAAGCTTGCGAAGACATTTTAACATTC
  ATTCTTGGGAGAAGAAGTATCAGTGCCGTTACTGTGATAAAGTATTTCCTCTTGCAGAATACCGCACCAA
  GCATGAAATCCATCACACAGGGGAGCGAAGGTATCAGTGTTTAACGTGTGGCAAATCTTTCATCAACTAT
  CAGTTTATGTCTTCACACATAAAGTCAGTTCATAGTCAAGATCCTTCTGGGGACTCAAAGCTTTATCGCT
  TACATCCATGCAAGTCTTTACAGATCAGACAGTATGCATACCTTTCTAATAAGTCAAGTGCTATGCCTGT
  CATGAAGGATGATGCTGTTGGATACAAGGTTGATGCTGGAAAAGAGCCTCCAGTAGGGACCACATCTACT
  CCTCCTCAGAACAAGTCAACGTTCTGGGAAGATATTTTTATTCAGCAAGAAAATGATTCCATTTTTAAAC
  AGAATGTAACCGATGGCAGTACTGAGTTTGAATTTATAATACCAGAATCTTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_020256
• Insert Size: 2016 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020256.2, NP_064652.2
• RefSeq Size: 5122 bp
• RefSeq ORF: 2016 bp
• Locus ID: 56805
• UniProt ID: Q8BN78
• Cytogenetics: X A3.3
• Gene Summary: Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). May recruit the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. Contributes to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. Represses expression of MMP7 in conjunction with transcriptional corepressors CBFA2T3, CBFA2T2 and RUNX1T1 (By similarity).[UniProtKB/Swiss-Prot Function]