Taf6 (NM_009315) Mouse Untagged Clone

CAT#: MC220328

Taf6 (untagged) - Mouse TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor (Taf6), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Taf6
• Synonyms: 80kDa; AW549759; p80; TAF(II)80; Taf2e; TAFII70
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220328 representing NM_009315
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGGAGAAGAAACTGAAACTCAGCAATACTGTGTTGCCCTCTGAGTCCATGAAGGTGGTGGCAG
  AATCTATGGGCATTGCTCAGATTCAGGAGGAGACTTGCCAGCTACTGACAGATGAGGTCAGCTACCGCAT
  CAAGGAGATTGCCCAGGATGCCCTGAAGTTCATGCACATGGGGAAACGACAGAAGCTCACTACCAGTGAC
  ATTGACTATGCATTGAAGTTAAAGAATGTGGAGCCACTGTATGGCTTCCATGCTCAGGAGTTCATCCCTT
  TCCGGTTTGCCTCTGGCGGGGGCCGGGAGCTGTACTTCTATGAAGAGAAGGAAGTGGACCTGAGTGACAT
  CATCAACACCCCTCTGCCTCGGGTGCCTCTGGATGTCTGCCTCAAAGCTCATTGGCTAAGCATCGAAGGC
  TGCCAGCCAGCTATACCAGAGAATCCACCCCCAGCTCCCAAAGAGCAGCAGAAGGCTGAGGCCACAGAAC
  CCCTGAAATCAGCAAAGCCAGGCCAAGAAGAAGATGGACCCCTGAAAGGCAAAGGTCAAGGGGCAGCTGC
  AGCTGATGGCAAAGGAAAAGAGAAGAAGGCACCTCCCTTGCTGGAGGGGGCCCCTTTCCGCCTGAAGCCC
  CGCAGCATCCATGAGCTGTCTGTGGAGCAGCAGCTGTATTACAAGGAGATCACGGAAGCCTGTGTGGGAT
  CCTGTGAGGCCAAGAGAGCGGAAGCTCTGCAGAGCATTGCCACGGACCCAGGGCTCTATCAGATGCTACC
  ACGGTTCAGTACCTTCATCTCAGAGGGGGTCCGTGTGAATGTAGTTCAGAATAACCTGGCCTTGCTTATC
  TACCTGATGCGTATGGTGAAGGCCCTGATGGACAACCCCACACTGTATCTAGAGAAATATGTGCATGAGC
  TCATTCCAGCAGTAATGACCTGCATTGTGAGTAGACAGCTGTGCTTGCGGCCAGATGTGGACAATCACTG
  GGCACTCCGGGATTTTGCTGCCCGTCTAGTGGCCCAAATCTGTAAGCATTTCAGCACAACCACCAACAAC
  ATCCAATCCCGAATCACTAAGACCTTTACCAAGAGCTGGGTCGACGAGAAGACTCCATGGACAACTCGAT
  ATGGCTCCATTGCAGGCCTAGCAGAGCTGGGACATGATGTGATTAAGACGCTAATTCTGCCAAGGCTGCA
  GCAGGAAGGGGAACGGATCCGCAGTGTCTTGGATGGGCCTGTGCTGAGCAACATCGACCGTATTGGAGCA
  GACCATGTGCAGAGCCTTCTCCTGAAACACTGTGCCCCAGTACTGGCCAAGCTGCGCCCTCCACCTGACA
  ATCAGGATGCCTACCGGGGAGAATTTGGGTCCCTTGGGCCCCTTCTCTGTTCCCATGTGGTGAAGGCCAG
  GGCCCAGGCTGCTCTACAAGCTCAGCAGGTCAACAGGACCACACTCACCATCACACAGCCTCGGCCCACA
  CTGACCCTTTCACAGGCCCCTCAGCCAGGCCCTCGCACGCCTGGCTTATTGAAGGTCCCTGGCTCCATTG
  CCTTGCCTGTTCAGACACTGGTGTCTGCGAGAGCTGCTGCTCCTCCTCAGCCTTCTCCTCCTCCAACCAA
  GTTTATTGTAATGTCCTCATCTTCCAGTGCCTCATCAACCCAGCAGGTTCTGTCCCTCAGCACCTCAGCT
  CCTGGCTCAGGCTCTACTACTACCTCTCCTGTCACCACCACGGTTCCCAGCGTGCAGCCCATCGTCAAGC
  TGGTCTCCACTGCTACCACTGCACCCCCAAGCACAGCCCCTGCTGGTTCTGGCAGTGTTCAGAAATACAT
  TGTGGTCTCTCTTCCCCCAACAGGGGAAGGCAAAGGAGGCCCTCCTTCTCATCCTTCTCCAGTCCCCCCT
  TCATCTTCTTCACCATCCCCACTTGGGGGAAGTACCCTCTGTGGGGGGAAGCAGGAAGCTGGGGACAGCC
  CCCCTCCAGCTCCAGGGACTCCAAAGGCTAATGGCTCGCAGCCTACTGGACCTGGCTCTCCCCAGCCTGC
  TCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009315
• Insert Size: 2037 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009315.3, NP_033341.1
• RefSeq Size: 2264 bp
• RefSeq ORF: 2037 bp
• Locus ID: 21343
• UniProt ID: Q62311
• Cytogenetics: 5 G2
• Gene Summary: TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All four transcripts encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.