Gls (NM_001081081) Mouse Untagged Clone

CAT#: MC220314

Gls (untagged) - Mouse glutaminase (Gls), nuclear gene encoding mitochondrial protein, transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gls
• Synonyms: 6330442B14; AI314027; B230365M23Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220314 representing NM_001081081
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCGGCTGCGAGGCTCGGCGATGCTACGGGAGCTGCTCTTAAGGCCGCCCGCCGCCGTCGGAGCCG
  TCCTGCGGCGCGCGCAGCCCCTCGGCACGCTGTGCCGGCGCCCCCGGGGCGGGAGCCGGCCGACCGCTGG
  CCTGGTGGCCGCTGCGCGACTCCACCCGTGGTGGGGCGGGGGCGGCCGCGCGAAGGGCCCCGGCGCGGGC
  GGCCTGTCCAGTTCGCCCTCGGAGATCCTACAGGAGCTGGGGAAGGGGGGTACGCCGCCACAGCAGCAGC
  AGCAGCAGCAGCAGCAGCCAGGGGCGTCGCCACCCGCAGCCCCGGGCCCCAAGGACAGCCCGGGGGAGAC
  CGACGCGTTCGGCAACAGCGAGGGCAAGGAGATGGTGGCCGCGGGCGACAATAAAATAAAACAGGGTCTG
  TTACCTAGCTTGGAAGATTTGCTGTTCTATACAATTGCAGAAGGACAAGAAAAGATACCTGTTCACAAGT
  TTATTACAGCACTCAAATCTACAGGATTGCGAACATCTGATCCCAGGTTGAAAGAGTGTATGGATATGTT
  AAGATTAACTCTTCAGACAACGTCAGATGGTGTCATGCTAGACAAAGATCTTTTTAAAAAGTGTGTTCAA
  AGCAACATTGTTTTGTTGACACAAGCATTTAGAAGAAAGTTTGTCATTCCTGACTTTATGTCTTTTACCT
  CACACATCGATGAGTTATATGAAAGTGCTAAAAAGCAGTCTGGAGGGAAGGTTGCTGATTATATTCCTCA
  GCTGGCCAAATTCAGTCCTGATTTGTGGGGTGTATCTGTCTGTACTGTAGATGGGCAAAGGCATTCTATT
  GGAGATACCAAAGTTCCTTTTTGTCTTCAGTCCTGTGTAAAACCCCTGAAATATGCAATTGCTGTTAATG
  ACCTGGGAACTGAGTATGTACATCGCTATGTTGGGAAGGAGCCAAGTGGATTAAGATTCAACAAACTCTT
  TTTGAATGAAGATGATAAACCACATAATCCTATGGTAAATGCTGGAGCAATTGTTGTGACTTCTCTAATA
  AAGCAAGGAGTAAATAATGCTGAGAAGTTTGACTACGTGATGCAATTTTTGAATAAGATGGCTGGTAATG
  AATATGTTGGATTCAGTAATGCAACGTTTCAGTCTGAACGAGAAAGTGGAGATCGAAATTTTGCAATAGG
  ATATTACTTAAAAGAAAAGAAGTGTTTTCCAGAAGGCACAGACATGGTTGGGATACTAGATTTTTACTTC
  CAGCTGTGCTCTATTGAAGTGACATGTGAATCAGCAAGTGTGATGGCTGCCACCTTGGCTAATGGTGGTT
  TCTGCCCAATTACTGGTGAAAGAGTCCTCAGTCCTGAGGCAGTTCGGAATACACTGAGCTTGATGCATTC
  TTGTGGCATGTATGACTTCTCAGGGCAGTTTGCGTTCCATGTTGGTCTTCCTGCAAAATCTGGAGTTGCT
  GGGGGTATTCTTTTAGTTGTCCCCAACGTCATGGGCATGATGTGTTGGTCTCCTCCTCTTGACAAGATGG
  GCAACAGTGTTAAGGGAATTCACTTTTGTCACGATCTTGTTTCTCTGTGTAACTTCCATAACTATGATAA
  TTTGAGACACTTTGCAAAAAAACTTGATCCTCGGAGAGAAGGAGGTGATCAAAGGGTGAAGTCGGTGATA
  AACCTTCTGTTTGCCGCATACACTGGAGATGTGTCTGCCCTCCGAAGGTTTGCTCTGTCAGCCATGGACA
  TGGAGCAGCGGGATTATGACTCCAGAACAGCCCTCCATGTCGCAGCAGCAGAGGGTCATGTTGAAGTTGT
  CAAGTTTTTGCTGGAAGCTTGCAAAGTAAACCCTTTCCCCAAGGACAGGTGGAATAATACCCCCATGGAT
  GAAGCACTACACTTTGGACACCATGATGTTTTTAAAATCCTTCAGGAATACCAAGTTCAGTACACACCTC
  AAGGGGATTCTGATGATGGAAAGGGAAACCAGACTGTCCACAAGAATCTCGACGGGTTGCTATAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001081081
• Insert Size: 2025 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081081.2, NP_001074550.1
• RefSeq Size: 4974 bp
• RefSeq ORF: 2025 bp
• Locus ID: 14660
• UniProt ID: D3Z7P3
• Cytogenetics: 1 26.86 cM
• Gene Summary: Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.