Rfx6 (NM_177306) Mouse Untagged Clone

CAT#: MC220294

Rfx6 (untagged) - Mouse regulatory factor X, 6 (Rfx6), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rfx6
• Synonyms: 4930572O07Rik; Rfxdc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220294 representing NM_177306
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTACAAAACTCACTGCCAGTGCATACTCGACAATGCCATCAATGGGAACTTTGAAGAGATCCAGC
  ATTTCTTACTACACTTTTGGCAAGGAATGCCAGACCATCTCCTTCCCCTGCTTGAAAATCCTGTTATCAT
  TGATATTTTCTGTGTCTGTGACTCAATTCTTTATAAGGTTCTTACAGATGTCCTCATTCCTGCAACAATG
  CAAGAAATGCCTGAAAGTTTGTTAGCAGATATAAGAAATTTTGCTAAAAATTGGGAACAGTGGGTTGTTT
  CATCCTTGGAAAACTTGCCAGAAGCCCTCATTGATAAGAAAATCCCCATTTTGCGAAGATTTGTATCTTC
  CCTGAAGCGACAGACATCTTTCTTGCATCTTGCTCAGATTGCCAGACCAGCTCTCTTTGACCAGCATGTG
  GTGAATGCCATGGTATCTGATATTGAAAAGGTTGACTTAAATAGTATTGGGTCTCAGGCTCTTCTTACCA
  TATCCAACAGCACAGACACGGAATCTGACATCTACAGTGAACATGACTCTATTACTGTGTTCCAAGAACT
  GAAAGATCTCCTTAAGAAGAATGCTACAGTGGAGGCATTTATTGAATGGTTGGACACTGTGGTAGAGCAG
  AGAGTTATTAAGATGAGCAAACAAAATGGAAGATCTCTGAAGAAGAGGGCTCAAGACTTTCTGCTCAAAT
  GGAGCTTTTTTGGTGCCCGCGTGATGCATAATCTTACCTTGAACAACGCATCAAGTTTTGGCTCTTTCCA
  TCTGATCCGAATGCTTCTGGATGAGTACATTCTCCTGGCCATGGAGACTCAATTTAACAATGACAAAGAG
  CAGGAACTACAGAATTTATTGGACAAGTATATGAAGAACTCGGATGCGAGTAAAGCTGCCTTCACAGCTT
  CCCCGAGCTCTTGCTTTCTGGCCAACCGAAATAAGGCTAGCTCACTTGCCAGTGACACTGTGAAGAACGA
  AAGCCACGTGGAGACATCCTATGTCCCTCTGCCTTCCAGCCAGCCTGGAGCCATACCCCCTGCTCTGCAC
  CCATTCTCAACTGAGGACACTGATAACATGCCACTCCCAGGTCAAATAGAGCTTTCACAAAGTACTGGCC
  ATCTGATGACACCACCGATTTCTCCAGCCATAGCAAGCAGAGGAAGTGTTATTAACCAAGGGCCAATGGC
  GAGCAGACCCCCGAGCGTGGGCACAGTTCTCTCAGCTCCAACACATTGCTCAACATATGCAGAACCAATT
  TATCCTACGCTCTCTCCAGCCAACCACGACTTTTATGGGACCAACTCTAACTATCAGACTATGTTTAGGA
  CACAGTCTCACCCTGCATCAAGCCTCTATGCTCACCGTGCAGAGCATGGGCGGTGCATGGCCTGGACTGA
  ACAGCAGCTTTCTAGAGACTTCTTTGGTGGCAGTTGTGCTGGGTCTCCATATAATTGTAGGCCACCTTCC
  AGTTATGGACCATCCACACACACACAAGAGTCACACAGCATGCAGGTTTTGAACACAGGAAGCTTCAATT
  TCCTTAGTAATGCAGGAGCTGGCAGCTGCCAAGGGTCAACATTGCCTTCTAATTCTCCCAATGGATACTA
  TGGAAACAATATAAACTACTCAGAGGCACATAGGCTTGGATCGATGGTGAACCAACATGTTTCAGTCATC
  AGCAGTGTGCGCTCCCTGCCTCCCTACAGTGATATTCATGATCCACTTAACATTTTAGATGACAGCAGCC
  GGAAGCAGAACAACTCGTTTTATGCAGACACATTGTCTCCTGTTGCATGTCGTACTACTGTAGTAGCTTC
  CAACTTGCAAACCCAGATTCCTTCATCTTCATCCCAGTGTATGTATGGAACTTCCAATCAGTATCCAGTG
  CAAGATAGTCTGGACTCCAATGCAGCAAGCAACAGAGAAATGGTGTCCTCTTTACCACCCATCAACACCG
  TGTTTATGGGGACAGCAGCTGGAGACACTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177306
• Insert Size: 1992 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177306.3, NP_796280.1
• RefSeq Size: 3088 bp
• RefSeq ORF: 1992 bp
• Locus ID: 320995
• UniProt ID: Q8C7R7
• Cytogenetics: 10 B3
• Gene Summary: Transcription factor required to direct islet cell differentiation during endocrine pancreas development. Specifically required for the differentiation of 4 of the 5 islet cell types and for the production of insulin. Not required for pancreatic PP (polypeptide-producing) cells differentiation. Acts downstream of NEUROG3 and regulates the transcription factors involved in beta-cell maturation and function, thereby restricting the expression of the beta-cell differentiation and specification genes, and thus the beta-cell fate choice. Activates transcription by forming a heterodimer with RFX3 and binding to the X-box in the promoter of target genes (PubMed:20148032). Involved in glucose-stimulated insulin secretion by promoting insulin and L-type calcium channel gene transcription (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1.