Atf6 (NM_001081304) Mouse Untagged Clone

CAT#: MC220203

Atf6 (untagged) - Mouse activating transcription factor 6 (Atf6), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atf6
• Synonyms: 9130025P16Rik; 9630036G24; AA789574; Atf6alpha; ESTM49
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220203 representing NM_001081304
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCGCCTTTTAGTCCGGTTCTTCCTCATGGACCAGATGAAGACTGGGAGTCGACGTTGTTTGCTG
  AACTTGGCTATTTCACAGACACTGATGATGTGCACTTTGATGCAGCACATGAGGCTTATGAAAATAATTT
  TGATCATCTTAATTTTGATTTGGATTTGATGCCTTGGGAGTCAGACCTATGGAGCCCCGGCAGCCACTTC
  TGCTCAGACATGAAGGCAGAGCCCCAGCCTCTTTCTCCGGCTTCCTCCAGTTGCTCCATCTCCTCTCCTC
  GGTCCACAGACTCGTGTTCTTCAACTCAGCACGTTCCTGAGGAGTTGGATTTGTTGTCTAGTTCTCAGTC
  CCCCCTTTCCTTATATGGCGACAGCTGTAATAGCCCCTCCTCTGTAGAGCCACTGAAGGAAGAGAAGCCT
  GTCACTGGTCCTGGAAACAAAACAGAACATGGACTGACTCCAAAGAAAAAAATTCAGATGAGTTCAAAAC
  CTTCAGTTCAGCCCAAGCCTTTATTACTTCCAGCAGCGCCCAAGACTCAAACCAATGCCAGTGTCCCAGC
  AAAAGCCATCATCATTCAGACACTACCAGCCCTTATGCCACTGGCAAAGCAGCAGTCGATTATCAGCATA
  CAGCCTGCGCCCACCAAAGGCCAGACTGTTTTGCTCTCTCAGCCGACTGTGGTTCAACTTCAGAGCCCTG
  CGGTTCTGTCGTCTGCTCAGCCGGTTCTTGCAGTCACTGGGGGAGCCGCACAGCTACCTAACCATGTGGT
  GAATGTGCTGCCAGCCCCTGTGGTGAGCAGCCCGGTGAATGGAAAACTTTCCGTGACTAAACCTGTTCTA
  CAAAGTGCCACCAGAAGTATGGGTTCGGATATCGCTGTGCTGAGGAGACAGCAGCGGATGATAAAGAACC
  GAGAGTCTGCTTGTCAGTCGCGCAAGAAGAAGAAAGAGTATATGCTAGGACTGGAGGCCAGGCTCAAGGC
  TGCCCTCTCAGAGAATGAGCAGCTGAAGAAGGAGAATGGCTCCCTGAAGCGACAGCTGGACGAGGTGGTG
  TCAGAGAACCAGAGGCTCAAAGTCCCAAGTCCAAAGCGAAGAGCTGTCTGTGTGATGATAGTATTAGCAT
  TTATAATGCTGAACTATGGGCCCATGAGCATGCTGGAGCAAGAATCCCGAAGAGTGAAACCTAGTGTGAG
  CCCTGCCAATCAGAGGAGGCATCTCTTGGAATTTTCAGCAAAAGAAGTTAAAGACACATCAGATGGTGAC
  AACCAGAAAGACAGTTACAGCTATGATCACTCTGTGTCCAATGACAAAGCTTTAATGGTGCTAAGTGAAG
  AGCCATTGCTTTATATGCCTCCACCTCCATGTCAACCCCTGATTAACACAACAGAGTCTCTCAGGTTGAA
  CCATGAACTTCGAGGCTGGGTTCATAGACATGAAGTGGAAAGGACCAAATCTAGAAGAATGACAAATAGC
  CAACAGAAAGCCCGCATTCTCCAGGGTGCTCTGGAACAGGGCTCTAATTCTCAGCTGATGGCTGTCCAGT
  ACACAGAAACCACTAGCATCAGTAGGAATTCTGGGAGTGAGCTGCAAGTGTATTACGCCTCCCCTGGAAG
  TTACCAAGGCTTCTTTGACGCCATCCGCAGGAGGGGAGATACGTTTTACGTTGTCTCATTTCGAAGGGAT
  CATCTGCTATTACCAGCTACCACCCACAACAAGACCACAAGACCAAAAATGTCAATTGTATTACCAGCAA
  TAAACATAAATGATAATGTGATCAATGGGCAGGACTATGAAGTAATGATGCAGATTGACTGTCAGGTGAT
  GGACACCAGGATCCTCCACATCAAAAGCTCCTCGGTTCCCCCTTATCTCCGGGATCATCAGCGGAACCAA
  ACCAGCACCTTCTTTGGTTCCCCTCCAACAACCACAGAGACGACCCATGTGGTCAGCACCATCCCTGAGT
  CGTTGCAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081304
• Insert Size: 1971 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081304.1, NP_001074773.1
• RefSeq Size: 7463 bp
• RefSeq ORF: 1971 bp
• Locus ID: 226641
• UniProt ID: F6VAN0
• Cytogenetics: 1 76.96 cM
• Gene Summary: Transmembrane glycoprotein of the endoplasmic reticulum that functions as a transcription activator and initiates the unfolded protein response (UPR) during endoplasmic reticulum stress. Cleaved upon ER stress, the N-terminal processed cyclic AMP-dependent transcription factor ATF-6 alpha translocates to the nucleus where it activates transcription of genes involved in the UPR. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina (By similarity).[UniProtKB/Swiss-Prot Function]