Pip5k1c (NM_008844) Mouse Untagged Clone

CAT#: MC220165

Pip5k1c (untagged) - Mouse phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma (Pip5k1c), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pip5k1c
• Synonyms: AI115456; AI835305; Pip5kIgamma
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NM_008844.2
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTAGAGGTGCCGGACGAGGCGGAGAGCGCCGAAGCGGGGGCCGTGACGGCGGAAGCGGCCTGGT
  CTGCGGAGAGTGGGGCGGCGGCAGGTATGACCCAGAAGAAGGCTGGCCTTGCAGAGGCCCCCTTGGTGAC
  AGGGCAGCCAGGCCCTGGCCATGGGAAGAAGCTGGGCCACCGAGGCGTGGATGCGTCGGGAGAGACTACG
  TATAAGAAGACCACCTCGTCCACCCTGAAGGGCGCCATCCAGCTGGGGATCGGGTACACGGTGGGCAACC
  TGAGCTCTAAGCCGGAGCGGGACGTGCTCATGCAGGACTTCTACGTGGTGGAGAGCATCTTCTTCCCCAG
  TGAAGGGAGCAACCTCACCCCTGCCCACCACTTCCAGGATTTCCGCTTCAAGACCTATGCACCTGTTGCC
  TTCCGCTACTTCCGGGAGCTCTTTGGCATCCGTCCAGATGACTATTTGTACTCCCTGTGCAATGAGCCAC
  TCATCGAGCTCTCCAACCCCGGGGCCAGCGGCTCTGTCTTCTACGTCACCAGCGACGACGAGTTCATCAT
  CAAGACTGTCATGCACAAGGAGGCAGAGTTCCTGCAGAAGCTGCTGCCTGGCTACTACATGAATCTCAAC
  CAGAACCCACGGACGCTGCTGCCCAAGTTCTATGGGCTGTACTGCGTGCAGTCTGGTGGCAAGAACATCC
  GCGTGGTGGTCATGAACAATGTGCTGCCCCGTGTCGTCAAAATGCACCTTAAGTTCGACCTCAAGGGCTC
  CACGTACAAGCGCAGGGCCAGCAAGAAGGAGAAGGAGAAGAGCCTGCCCACCTACAAGGACCTGGACTTC
  ATGCAGGACATGCCCGAGGGGCTGCTGCTGGACTCCGACACCTTTGGCGCCCTGGTCAAGACGCTGCAGC
  GAGACTGCCTGGTGCTGGAGAGCTTCAAGATAATGGACTACAGCCTGCTGCTGGGCGTGCACAACATCGA
  TCAGCAGGAGCGAGAGCGCCAGGCCGAGGGCGCCCAGAGCAAGGCGGATGAGAAGCGGCCCGTGGCCCAG
  AAGGCTCTGTATTCCACGGCCATGGAGTCTATCCAGGGCGGAGCTGCCCGTGGGGAGGCCATTGAGACAG
  ATGACACGATGGGTGGGATTCCAGCAGTGAATGGGCGCGGGGAGCGACTGCTCCTCCACATCGGGATCAT
  TGATATTCTGCAGTCCTACAGGTTCATCAAGAAGTTAGAACACACCTGGAAGGCCCTCGTCCATGATGGG
  GACACTGTCTCAGTCCACCGGCCCAGCTTCTATGCAGAGCGCTTCTTCAAGTTCATGAGCAGCACGGTGT
  TCCGGAAGAGTTCCTCCCTGAAGTCCTCTCCATCCAAGAAAGGACGTGGTGCCCTGCTGGCGGTCAAACC
  CCTGGGGCCCACTGCTGCCTTCTCAGCCAGCCAGATCCCCAGCGAGAGAGAGGATGTGCAGTATGACCTG
  CGGGGGGCCCGCAGCTACCCCACGCTTGAGGATGAAGGCCGGCCTGACCTCCTGCCCTGCACCCCACCGT
  CCTTTGAGGAAGCCACCACAGCCTCCATCGCCACCACCCTGTCGTCCACCTCCCTCTCCATCCCAGAGCG
  GTCCCCTTCAGATACATCGGAGCAGCCCCGGTACAGGCGGCGCACGCAGTCTTCAGGCCAGGATGGCCGG
  CCCCAGGAGGAGCCCCATGCGGAAGACCTGCAGAAGATAACTGTGCAGGTGGAGCCAGTGTGCGGTGTGG
  GGGTTGTCCCCAAGGAGGAGGGTGCAGGAGTGGAGGTCCCCCCATGTGGGGCATCGGCTGCAGCCTCTGT
  GGAAATAGACGCTGCCAGCCAGGCCTCAGAGCCTGCCAGCCAGGCCTCAGATGAGGAGGATGCACCCTCT
  ACAGACATCTATTTTCCCACCGACGAGAGGAGCTGGGTGTACTCCCCGCTTCACTATAGCGCGCGGCCCG
  CCTCCGACGGCGAGAGCGACACATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008844
• Insert Size: 1986 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008844.2, NP_032870.2
• RefSeq Size: 4287 bp
• RefSeq ORF: 1986 bp
• Locus ID: 18717
• UniProt ID: O70161
• Cytogenetics: 10 C1
• Gene Summary: Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as vesicle mediated transport, cell adhesion, cell polarization and cell migration. Together with PIP5K1A is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle attachment by generating the pool of PtdIns(4,5)P2 that induces controlled actin depolymerization to facilitate Fc-gamma-R clustering. Mediates RAC1-dependent reorganization of actin filaments. Required for synaptic vesicle transport. Controls the plasma membrane pool of PtdIns(4,5)P2 implicated in synaptic vesicle endocytosis and exocytosis. Plays a role in endocytosis mediated by clathrin and AP-2 (adaptor protein complex 2). Required for clathrin-coated pits assembly at the synapse. Participates in cell junction assembly. Modulates adherens junctions formation by facilitating CDH1 trafficking. Required for focal adhesion dynamics. Modulates the targeting of talins (TLN1 and TLN2) to the plasma membrane and their efficient assembly into focal adhesions. Regulates the interaction between talins (TLN1 and TLN2) and beta-integrins. Required for uropodium formation and retraction of the cell rear during directed migration. Has a role in growth factor- stimulated directional cell migration and adhesion. Required for talin assembly into nascent adhesions forming at the leading edge toward the direction of the growth factor. Negative regulator of T-cell activation and adhesion. Negatively regulates integrin alpha-L/beta-2 (LFA-1) polarization and adhesion induced by T-cell receptor. Together with PIP5K1A has a role during embryogenesis and together with PIP5K1B may have a role immediately after birth.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) lacks an alternate in-frame exon in the 3' coding region, compared to variant 3. The encoded protein (isoform 1) is shorter, compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.