Man1a (NM_008548) Mouse Untagged Clone

CAT#: MC220160

Man1a (untagged) - Mouse mannosidase 1, alpha (Man1a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Man1a
• Synonyms: Man1a1; PCR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220160 representing NM_008548
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGTGGGGGGCCTGTTGCCGCTCTTCAGTAGCCCTGGGGGCGGCGGCCTGGGCAGTGGCCTGGGCG
  GGGGGCTTGGCGGCGGGAGGAAGGGGTCTGGCCCCGCTGCCTTCCGCCTCACCGAGAAGTTCGTGCTGCT
  GCTGGTGTTCAGCGCCTTCATCACGCTCTGCTTCGGGGCAATCTTCTTCCTGCCTGACTCCTCCAAGCTG
  CTCAGCGGGGTCCTGTTCCACTCCAACCCTGCCTTGCAGCCGCCGGCGGAGCACAAGCCCGGGCTCGGGG
  CGCGTGCGGAGGATGCCGCCGAGGGGAGAGTCCGGCACCGCGAGGAAGGCGCGCCTGGGGACCCTGGAGC
  TGGACTGGAAGACAACTTAGCCAGGATCCGCGAAAACCACGAGCGGGCTCTCAGGGAAGCCAAGGAGACC
  CTGCAGAAGCTGCCGGAGGAGATCCAAAGAGACATTCTGCTGGAGAAGGAAAAGGTGGCCCAGGACCAGC
  TGCGTGACAAGGATCTGTTTAGGGGCTTGCCCAAGGTGGACTTCCTGCCCCCCGTCGGGGTAGAGAACCG
  GGAGCCCGCTGACGCCACCATCCGTGAGAAGAGGGCAAAGATCAAAGAGATGATGACCCATGCTTGGAAT
  AATTATAAACGCTATGCGTGGGGCTTGAACGAACTGAAACCTATATCAAAAGAAGGCCATTCAAGCAGTT
  TGTTTGGCAACATCAAAGGAGCTACAATAGTAGATGCCCTGGATACCCTTTTCATTATGGGCATGAAGAC
  TGAATTTCAAGAAGCTAAATCGTGGATTAAAAAATATTTAGATTTTAATGTGAATGCTGAAGTTTCTGTT
  TTTGAAGTCAACATACGCTTCGTCGGTGGACTGCTGTCAGCCTACTATTTGTCCGGAGAGGAGATATTTC
  GAAAGAAAGCAGTGGAACTTGGGGTAAAATTGCTACCTGCATTTCATACTCCCTCTGGAATACCTTGGGC
  ATTGCTGAATATGAAAAGTGGGATCGGGCGGAACTGGCCCTGGGCCTCTGGAGGCAGCAGTATCCTGGCC
  GAATTTGGAACTCTGCATTTAGAGTTTATGCACTTGTCCCACTTATCAGGAGACCCAGTCTTTGCCGAAA
  AGGTTATGAAAATTCGAACAGTGTTGAACAAACTGGACAAACCAGAAGGCCTTTATCCTAACTATCTGAA
  CCCCAGTAGTGGACAGTGGGGTCAACATCATGTGTCGGTTGGAGGACTTGGAGACAGCTTTTATGAATAT
  TTGCTTAAGGCGTGGTTAATGTCTGACAAGACAGATCTCGAAGCCAAGAAGATGTATTTTGATGCTGTTC
  AGGCCATCGAGACTCACTTGATCCGCAAGTCAAGTGGGGGACTAACGTACATCGCAGAGTGGAAGGGGGG
  CCTCCTGGAACACAAGATGGGCCACCTGACGTGCTTTGCAGGAGGCATGTTTGCACTTGGGGCAGATGGA
  GCTCCGGAAGCCCGGGCCCAACACTACCTTGAACTCGGAGCTGAAATTGCCCGCACTTGTCATGAATCTT
  ATAATCGTACATATGTGAAGTTGGGACCGGAAGCGTTTCGATTTGATGGCGGTGTGGAAGCTATTGCCAC
  GAGGCAAAATGAAAAGTATTACATCTTACGGCCCGAGGTCATCGAGACATACATGTACATGTGGCGACTG
  ACTCACGACCCCAAGTACAGGACCTGGGCCTGGGAAGCCGTGGAGGCTCTAGAAAGTCACTGCAGAGTGA
  ACGGAGGCTACTCAGGCTTACGGGATGTTTACATTGCCCGTGAGAGTTATGACGATGTCCAGCAAAGTTT
  CTTCCTGGCAGAGACACTGAAGTATTTGTACTTGATATTTTCCGATGATGACCTTCTTCCACTAGAACAC
  TGGATCTTCAACACCGAGGCTCATCCTTTCCCTATACTCCGTGAACAGAAGAAGGAAATTGATGGCAAAG
  AGAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008548
• Insert Size: 1968 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008548.4, NP_032574.1
• RefSeq Size: 3889 bp
• RefSeq ORF: 1968 bp
• Locus ID: 17155
• UniProt ID: P45700
• Cytogenetics: 10 B3
• Gene Summary: Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2).[UniProtKB/Swiss-Prot Function]