Kcna4 (NM_021275) Mouse Untagged Clone

CAT#: MC220156

Kcna4 (untagged) - Mouse potassium voltage-gated channel, shaker-related subfamily, member 4 (Kcna4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcna4
• Synonyms: Kv1.4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220156 representing NM_021275
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGTGGCGATGGTGAGTGCCGAGAGCTCAGGGTGCAACAGCCATATGCCTTATGGTTATGCAGCCC
  AGGCCAGGGCCCGAGAGCGGGAGAGACTCGCTCACTCCAGGGCAGCTGCAGCTGCTGCTGTCGCAGCTGC
  CACGGCTGCTGTCGAAGGCACTGGGGGTTCTGGTGGAGGCCCCCACCACCATCATCAGACACGTGGGGCC
  TACTCCTCCCATGATCCTCAAGGTAGCCGTGGAAGTAGAAGGAGGAGGCGTCAGCGAACTGAGAAGAAGA
  AACTCCACCACAGGCAGAGCAGTTTTCCTCATTGCTCAGACCTGATGCCCAGTGGCTCTGAAGAGAAGAT
  CCTGAGGGAGCTAAGCGAGGAAGAGGAAGACGAGGAGGAGGAAGAGGAGGAGGAGGAGGAGGGAAGGTTT
  TACTATAGTGAAGAAGACCATGGGGATGGGTGTTCGTACACAGACCTGCTGCCACAGGATGATGGGGGTG
  GTGGCGGCTACAGTTCAGTCCGCTATAGTGACTGCTGTGAACGTGTGGTGATAAATGTGTCTGGTCTACG
  CTTCGAAACCCAAATGAAAACTTTGGCCCAGTTTCCAGAAACTCTGTTGGGAGACCCTGAGAAGAGGACT
  CAGTACTTCGACCCTTTGCGCAATGAGTATTTTTTTGATCGGAACCGACCCAGCTTTGATGCCATTTTGT
  ATTATTACCAGTCAGGAGGCCGCCTGAAGAGACCAGTCAATGTCCCCTTTGATATCTTCACCGAGGAGGT
  GAAGTTCTATCAGTTGGGAGAGGAAGCCCTGCTCAAGTTCCGGGAGGATGAGGGCTTTGTGAGAGAAGAG
  GAGGACAGGGCTCTGCCAGAAAATGAATTTAAAAAACAGATTTGGCTTCTCTTTGAATATCCAGAGAGTT
  CTAGCCCTGCCAGGGGTATAGCCATTGTGTCTGTCCTGGTCATCTTAATCTCTATTGTCATATTTTGCCT
  GGAAACCTTGCCGGAGTTCAGGGATGATAGGGACCTTATTATGGCCCTCAGTGCAGGCGGGCACAGCAGA
  TTGCTGAATGACACCTCGGCACCCCACCTGGAGAACTCAGGGCACACAATATTCAATGACCCTTTCTTCA
  TCGTGGAGACAGTGTGTATTGTGTGGTTTTCCTTTGAGTTTGTGGTTCGATGCTTTGCTTGTCCCAGCCA
  AGCACTCTTCTTCAAAAACATCATGAACATCATTGATATCGTCTCCATTTTGCCTTACTTCATCACTCTG
  GGCACTGACCTGGCCCAACAGCAGGGGGGTGGCAATGGCCAGCAGCAGCAGGCCATGTCCTTTGCCATCC
  TTAGGATCATTCGTCTGGTCCGAGTATTCCGGATCTTCAAGCTCTCCAGACACTCCAAAGGCCTGCAGAT
  CCTGGGCCACACCCTAAGAGCCAGCATGCGGGAACTGGGCCTTCTTATCTTTTTCCTCTTCATCGGGGTT
  ATCCTCTTTTCCAGCGCTGTGTATTTTGCAGAGGCGGATGAACCCACTACCCATTTCCAAAGCATTCCAG
  ATGCGTTTTGGTGGGCTGTGGTAACCATGACAACTGTGGGCTATGGGGACATGAAGCCCATCACAGTCGG
  GGGAAAGATTGTGGGGTCCCTGTGTGCCATTGCGGGTGTCTTAACCATTGCTTTGCCTGTGCCGGTGATT
  GTGTCTAACTTTAACTATTTCTACCACAGAGAGACTGAAAATGAAGAACAGACCCAGCTGACACAAAACG
  CAGTCAGTTGTCCATACCTACCTTCTAATTTGCTCAAGAAATTTCGGAGCTCCACTTCTTCTTCCCTGGG
  GGACAAGTCAGAGTATCTAGAGATGGAAGAAGGGGTCAAGGAATCATTATGTGGAAAGGAGGAGAAGTGT
  CAGGGAAAGGGAGATGAGAGCGAGACAGATAAAAACAACTGTTCTAATGCAAAGGCTGTGGAGACTGATG
  TGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021275
• Insert Size: 1965 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021275.4, NP_067250.2
• RefSeq Size: 4844 bp
• RefSeq ORF: 1965 bp
• Locus ID: 16492
• UniProt ID: Q61423
• Cytogenetics: 2 56.12 cM
• Gene Summary: Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane (PubMed:8020965). Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel (By similarity). Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation. In vivo, membranes probably contain a mixture of heteromeric potassium channel complexes, making it difficult to assign currents observed in intact tissues to any particular potassium channel family member. Homotetrameric KCNA4 forms a potassium channel that opens in response to membrane depolarization, followed by rapid spontaneous channel closure (PubMed:8020965). Likewise, a heterotetrameric channel formed by KCNA1 and KCNA4 shows rapid inactivation (By similarity).[UniProtKB/Swiss-Prot Function]