Acox1 (NM_015729) Mouse Untagged Clone

CAT#: MC220142

Acox1 (untagged) - Mouse acyl-Coenzyme A oxidase 1, palmitoyl (Acox1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Acox1
• Synonyms: A; Acox; AOX; D130055E20Rik; Paox
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220142 representing NM_015729
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCCCGATCTGCGCAAGGAGCGGGCCGCCGCCACCTTCAATCCAGAGTTAATCACGCACATCTTGG
  ATGGTAGTCCGGAGAACACCCGGCGCCGTCGAGAAATCGAGAACTTGATTCTGAACGATCCAGACTTCCA
  ACATGAGGACTATAACTTCCTCACTCGAAGCCAGCGTTACGAGGTGGCTGTTAAGAAGAGTGCCACCATG
  GTGAAGAAGATGAGGGAATTTGGCATCGCAGACCCTGAAGAAATCATGTGGTTTAAAAACTCTGTGCACC
  GAGGGCATCCTGAGCCTTTGGACCTTCACTTGGGCATGTTCCTGCCCACCTTGCTTCACCAGGCCACCGA
  AGAGCAGCAGGAGCGTTTCTTCATGCCGGCCTGGAATCTGGAGATCACGGGCACTTATGCGCAGACAGAG
  ATGGGTCATGGAACTCATCTTCGAGGCTTGGAAACCACTGCCACATATGACCCCAAGACCCAAGAGTTCA
  TTCTCAACAGCCCAACTGTGACTTCCATCAAGTGGTGGCCTGGGGGGCTTGGGAAGACTTCCAATCATGC
  GATAGTCCTGGCTCAGCTCATCACTCGAGGGGAGTGCTACGGGTTACATGCCTTTGTTGTCCCTATCCGT
  GAGATTGGGACCCACAAGCCTCTGCCAGGCATCACTGTTGGGGATATCGGCCCCAAGTTTGGTTATGAAG
  AGATGGATAATGGCTACCTGAAGATGGACAATTACCGTATTCCTAGAGAGAACATGTTGATGAAATATGC
  CCAGGTGAAGCCTGACGGCACGTATGTAAAACCTCTGAGTAACAAGCTGACATATGGGACCATGGTTTTC
  GTAAGGTCCTTCCTCGTGGGAAGTGCAGCTCAGAGTCTGTCCAAGGCATGCACCATTGCCATTCGATACA
  GTGCTGTGAGGCGCCAGTCTGAAATCAAGAGAAGCGAGCCAGAGCCCCAGATTTTGGATTTTCAGACGCA
  GCAGTATAAACTCTTCCCGCTCCTGGCCACCGCCTATGCCTTCCACTTTCTCGGAAGATACATAAAGGAG
  ACCTACATGCGGATTAATGAGAGCATTGGCCAAGGCGACCTGAGTGAGCTGCCTGAGCTTCATGCCCTCA
  CAGCTGGGCTGAAGGCTTTTACTACCTGGACAGCCAATGCTGGTATCGAAGAATGTCGGATGGCTTGCGG
  TGGGCACGGCTATTCTCACAGCAGTGGGATTCCAAATATTTACGTCACGTTTACCCCGGCCTGCACCTTC
  GAGGGGGAGAACACTGTTATGATGCTGCAGACGGCCAGGTTCTTGATGAAAATCTATGACCAGGTTCAGT
  CGGGGAAGCTGGTGGGTGGTATGGTGTCGTACTTGAATGACCTGCCGAGCCAGCGTATCCAGCCGCAGCA
  GGTGGCAGTCTGGCCAACTCTGGTGGACATTAACAGCCTGGACAGCCTGACAGAAGCCTACAAGCTACGT
  GCAGCCAGATTGGTAGAAATTGCTGCAAAAAACCTTCAGGCCCAAGTGAGTCACAGGAAGAGCAAGGAAG
  TGGCGTGGAACTTGACTTCTGTCGACCTTGTTCGCGCAAGTGAGGCGCACTGCCACTACGTGACCGTTAA
  GGTCTTTGCAGATAAACTCCCCAAGATTCAAGACAGAGCCGTGCAAGCCGTGCTGAGGAACCTGTGTCTC
  TTATATTCTCTCTATGGGATCAGCCAGAAAGGAGGGGATTTTCTTGAGGGGAACATCATCACAGGGGCTC
  AGATGTCACAGGTAAACAGTCGGATCCTGGAGCTGCTCACAGTGACTCGCCCCAACGCTGTGGCTTTGGT
  GGATGCCTTTGACTTTAAGGATGTGACCCTTGGCTCTGTTCTCGGCCGCTATGATGGCAATGTGTATGAA
  AACTTGTTTGAGTGGGCCAAGAAGTCCCCACTGAACAAGACAGAGGTCCACGAATCTTACTACAAGCACT
  TGAAGCCCCTGCAGTCGAAGCTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_015729
• Insert Size: 1986 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015729.3, NP_056544.2
• RefSeq Size: 3992 bp
• RefSeq ORF: 1986 bp
• Locus ID: 11430
• UniProt ID: Q9R0H0
• Cytogenetics: 11 E2
• Gene Summary: This gene encodes a member of the acyl-coenzyme A oxidase family. The encoded protein is localized to peroxisomes and is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-coenzyme A to 2-trans-enoyl-coenzyme A. Disruption of this gene results in microvesicular steatohepatitis, spontaneous peroxisome proliferation, and the eventual development of hepatocellular carcinomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]
  Transcript Variant: This variant (1) encodes isoform 1.