Med17 (NM_144933) Mouse Untagged Clone

CAT#: MC220124

Med17 (untagged) - Mouse mediator complex subunit 17 (Med17), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Med17
• Synonyms: 77kDa; C330002H14Rik; Crsp6; Trap80
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220124 representing NM_144933
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGGCGTGCGAGCCGTGCGCATCAGCATCGAGTCGGCCTGCGAGAAGCAGGTGCAGGAGGTGGGCC
  TTGATGGCACCGAGACGTACCTGCAGCCGCTGTCCATGTCGCAGAACCTGGCGCGCCTGGCACAGCGCAT
  CGACTTCAGCCAGGGCTCTGGCTCTGAGGAGGAGGAGGCGGCAGGGCCTGACGGCGATGCGCCGGACTGG
  GGGGGCGCGGGAGCCGACCAGGACGACGAGGAGGGGTTGGTAAAATTCCAACCTTCTTTGTGGCCTTGGG
  ACTCTGTGAGGAACAATTTGCGAAGTGCCCTTACAGAGATGTGTGTTCTCTATGATGTCCTCAGTATTGT
  CAGGGACAAAAAGTTCATGACTCTGGACCCCGTCTCTCAGGATGCTCTTCCTCCCAAACAGAGTCCACAA
  ACGCTGCAGCTGATCTCCAAGAAGAAGTCCCTCGCTGGAGCAGCTCAGATCCTACTGAAGGGGGCAGAAA
  GACTGACCAAGTCAGTGGCTGAGAACCAGGAAAACAAGCTCCAGCGAGACTTCAATTCTGAGCTCCTGAG
  ACTCCGGCAGCACTGGAAGCTGAGGAAGGTGGGCGACAAGATCCTGGGTGACCTGAGCTACAGAAGTGCA
  GGTTCTCTCTTTCCTCATCATGGTACCTTTGAAGTGATAAAGAATACAGACATTGACTTGGATAAGAAGA
  TACCAGAAGATTACTGTCCTCTGGATGTCCAAATTCCTAGTGATTTAGAGGGGTCTGCGTATATCAAGGT
  TTCTATTCAGAAACAGGCTCCAGACATTGGTGATCTTGGCACAGTTAACCTTTTTAAGAGACCTTTGCCC
  AAATCCAAGCCAGGTTCCCCACACTGGCAGACAAAATTAGAAGCAGCACAAAATGTTCTTCTTTGTAAAG
  AAATTTTTGCACAGCTCTCCCGGGAAGCCGTTCAGATTAAGTCTCAGATCCCTCACATTGTGGTGAAAAA
  CCAGATCATCTCTCAGCCCTTTCCAAGCTTGCAGTTGTCCATTTCTCTGTGCCACTCCTCAGATGATAAG
  AAGTCGCAGAAGTGTGCCGCAGAGAAGCCTGGGCAAGAGGATCACCTCTACGTCCTGGAGCACAACCTGC
  ACCTGCTGATCAGAGAGTTTCATAAACAGACCTTGAGTTCCATTGTGATGCCACACCCAGCAAGTGCTCC
  CTTTGGCCACAAGAGGATGAGACTCTCAGGTCCTCAAGCTTTTGATAAAAATGAAATTAATTCAATACAG
  TCCACAGAAGGACTCCTAGAAAAAATAATTAAACAGGCAAAGCATATTTTTCTAAGGAGCAGAACTGCTG
  CAACCATTGACAGCTTGGCAAGTCGCATTGAGGACCCCCAGATCCAGGCCCACTGGTCAAACATCAATGA
  TGTGTATGAGTCTAGTGTAAAAGTTTTGATCACATCTCAGGGTTATGAACAGATCTGCAAGTCCATCCAG
  CTGCAGTTGAACATTGGTGTGGAGCAGGTCCGTGTTGTGCACAGAGATGGTCGGGTAATCATGTTGTCTC
  ATCAGGAGCAGGAGCTACAGGACTTCCTTCTGTCTCAGATGTCACAGCACCAGGTGCACGCGGTTCAGCA
  GCTTGCCAAGGTTATGGGCTGGCAGGTCCTCAGCTTCAGTAACCATGTGGGACTTGGGCCCATAGAGAGC
  ATTGGCAATGCTTCGGCCATCACCGTGGCCTCGCCAAGTGGAGACTATGCGATTTCAGTTCGTAACGGAC
  CTGAGAGTGGCAGCAAGATTATGGTTCAGTTTCCCCGTAACCAGTGCAAAGACCTTCCGAAGAGTGATGT
  TTTACAAGACAGCAAGTGGAGTCATCTCCGAGGACCATTCAAGGAGGTTCAGTGGAACAAGATGGAAGGC
  CGAAACTTCGTTTATAAAATGGAGCTGCTTATGTCTGCCCTGAGCCCCTGTCTGCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_144933
• Insert Size: 1950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144933.1, NP_659182.1
• RefSeq Size: 3914 bp
• RefSeq ORF: 1950 bp
• Locus ID: 234959
• UniProt ID: Q8VCD5
• Cytogenetics: 9 A2
• Gene Summary: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).[UniProtKB/Swiss-Prot Function]