Pja2 (NM_144859) Mouse Untagged Clone

CAT#: MC220119

Pja2 (untagged) - Mouse praja 2, RING-H2 motif containing (Pja2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pja2
• Synonyms: AI447901; AL022700; mKIAA0438; Neurodap1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220119 representing NM_144859
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACAGTACACTGAAAAAGAGCCATCAGTAATGGATCAAGAATCCAGCAAGGCTGCTTGGCCTAAAC
  CAGCAGGAGGATATCAGACTATCACAGGCAGGAGATATGGAAGAAGACATGCGTATGTCAGTTTTAAGCC
  ATGTATGACCAGGCATGAAAGAAGCTTGGGTCGGGCTGGTGATGACTATGAAGTGTTGGAACTAGACGAT
  GTTCCAAAGGAGAACACCTCAGGTTCCAGTTCATTGGATCAAGTCCATCCTGCTTTACCAAATGAACCTA
  CAGTTGAAAAAAGTGAAACAGAAATTTCTACTTGTGGTCCAGCACTGAATCAAAGCACTGAGAGCAGTCC
  ATCCATTGCCACAGTATGTCATAGTGAGGAAGTCAGGGAGACCTTAGAAAGCAATACAAACCTTCATAAT
  CGCACTGAGACAGAGCATACTCCAGCAGTTTGTAATGTCTCAAGTGTCCAGAATGGAATTATGTTGGTTC
  ATACTGACTCTTATGATCCAGACAGCAAGCATGATGAGAATGGCTCTCTTCAACTTGGCGCTGAAGCTGT
  GGAAGGTGGTAGACATCAGAAGGGGTTAGGCCGTGCAGTCTTTGAGCTGGAAAATGGAGAGGCAGAGATC
  TACGCTGACCTGTCACCATCAGTCCCCTCTCTCAACGGTGAAATAAGTGAGGCGTTTGAAGAGCTAGATT
  CAGCACCTTTAGAGAAAAGTTCTACTGCCGATGCTGAGCTTGTCCATCAGAATGGGCAGGAATTTCAGAG
  GTCTTCTGAAGATGGCGTTGTTAGAAAGAGGCGACAAGACGATACTGATCAGGGAAGACAGACAGAAAAT
  TCAACTGAAGATGCAGACTGTGCTCCAGGCCATGTTGAGCAAAATACTAGTGATAGAGCCAACCACCATG
  GAAGTTCTCCTGAACAGGTAGTAAGGCCCAAAGTTAGAAAAGTGATAAGTTCAAGCCAGGTGGACCAAGA
  AATTGGTTTTAATAGGCACGAGGCTAAGCAGAGAAGCGTTCAGAGATGGAGAGAGGCTTTGGAAGTGGAG
  GAGTGCAGTTCAGATGACCCTATAATAAAGTGTGACGATTATGATGGAGACCACGACTGCATGTTCCTAA
  CTCCAACTTACTCAAGAGTTACACAAAGGGAGACAGAACGGAATCGTGTAACATCAGAAAATGGAGCTAC
  AGCTTCAGGACGGCAAGAATCTCGGGACAATGCCTTTTGGAATGCCTGCGGAGAGTATTACCAACTCTTT
  GACAAAGATGAAGACAGGGAACAGACATCCTTGGAAGAGGGAGAGATCCCTTGGTTACAGTACAATGAGG
  TCAATGAGAGCAGCAGCGATGAAGGGAATGAGCCTGCCAATGAGTTTGCACAGCCGGAAGCTTTCATGTT
  GGATGGGAACAACAACCTTGAGGACGACTCGAGCGTGAGTGAAGACTTGGATGTGGACTGGAGCCTGTTT
  GATGGTTTTGCAGACGGACTTGGAGTTGCTGAAGCTATTTCTTATGTGGATCCTCAGTTCCTTACCTACA
  TGGCACTAGAAGAGCGCTTAGCCCAGGCTATGGAGACTGCGCTGGCACATTTAGAGTCCCTTGCAGTAGA
  CGTTGAGGTGGCCAATCCACCTGCCAGTAAGGAAAGCATTGATGGGCTTCCAGAGACTCTGGTTCTAGAG
  GACCACACCGCCATCGGCCAGGAGCAGTGCTGTCCCATCTGCTGCAGCGAGTACATCAAGGATGACATTG
  CCACAGAGCTGCCTTGCCATCACTTCTTTCATAAGCCCTGTGTCTCCATCTGGCTACAGAAGTCCGGAAC
  ATGCCCTGTGTGCCGCCGTCACTTCCCACCTGCAGTTATTGACGCATCTGCAGCTGCTTCCTCTGACCCT
  GACCCTGATGCCTCGCCTGCAAATGACAATGCTGAAGAAGCCCCATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_144859
• Insert Size: 1938 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144859.2, NP_659108.1
• RefSeq Size: 4520 bp
• RefSeq ORF: 1938 bp
• Locus ID: 224938
• UniProt ID: Q80U04
• Cytogenetics: 17 E1.1
• Gene Summary: Has E2-dependent E3 ubiquitin-protein ligase activity. Responsible for ubiquitination of cAMP-dependent protein kinase type I and type II-alpha/beta regulatory subunits and for targeting them for proteasomal degradation. Essential for PKA-mediated long-term memory processes. Through the ubiquitination of MFHAS1, positively regulates the TLR2 signaling pathway that leads to the activation of the downstream p38 and JNK MAP kinases and promotes the polarization of macrophages toward the pro-inflammatory M1 phenotype.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (b) lacks an internal segment, compared to isoform a.