Efhc1 (NM_027974) Mouse Untagged Clone

CAT#: MC220098

Efhc1 (untagged) - Mouse EF-hand domain (C-terminal) containing 1 (Efhc1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Efhc1
• Synonyms: 1700029F22Rik; mRib72-1; myoclonin1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220098 representing NM_027974
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACCAACCCGGTGCACGGCCTGCCCTTTCTCCCGGGCTCGTCCTTTACAGACTCCACGAAAACAG
  CATTCCACCGAAGCCAGACACTCAACTACAGGAACGGCTATGCAGTTGTCCGACGTCCCACCATGGGGAT
  AGGTGGAGACCGGCTCCACTACAACCAGCTGTCTCAGGCCGAGCTGGATGAACTGGCCAATAAGGCACCC
  ATCTTAACTTACGGGCCACTCAAGCAAGCCCCACTTGCAGAGTTTGTTCCTGCGCATGTGGCTTTTGACA
  AAAAGGTGCTGAAATTTAGTGCCTACTTCCAAGAAGATGTTCCCATATCAATGGAGGAGCATTACCGAAT
  CCGCCACGTGAACATTTACTACTACCTAGAAGATGACAGCATGTCTGTCATCGAGCCCGTAGTGGAGAAC
  TCTGGGATCCCACAAGGCAAGCTAATCAAACGCCAGCGGTTCACCAAGAATGATATGGGAGACCACTACC
  ACTGGAAGGACCTCAACCGTGGAATCAACCTTACAGTGTACGGCAAAACCTTCCGAATTGTTGACTGTGA
  CCGATTCACTCAGGACTTTTTGGAAAGCCAAGGCATTGAATTAAATCCATCAGAGAAGATACCCCTGGAT
  CCTTACACTCAACTCCGGAAAGAACCTGTGCGCAAATATGTCACCCCGTCAGACTTTGACCAACTGAAAC
  AGTTTCTCACCTTTGACAAACAGGTCCTTCGATTCTATGCCATCTGGGACGACACGGATAGCTTGTTCGG
  GGAATGTCGTCACTACATCATTCATTACTACCTCATGGATGACACCGTGGAGATCAGAGAGGTCCACGAA
  CGGAACAACGGGAGAGACCCCTTCCCACTCCTCATGAACCGACAGCGTATGCCCAAAGTTTTGGTGGAGA
  ATGCAAAGAATTTCCCGAAGTGTGTGCTGGAAATCTCAGACCAAGAGGTGCTGGAGTGGTACACTGCCAA
  GGACTTCATTGTGGGGAAGCCGCTCACCATCCTGGGGAGGACCTTCTTCATCTACGACTGTGACCCATTC
  ACACGACAGTTCTACAAAGACAAGTTTGGAATGCCTGACTTACCACCAGTTGATGTGACCAAGAAGGAGC
  CACCTCCAGTAAAACAGGAGTTGCCTCCTTATAATGGCTATGGACTAATTGAAGACTCTGCTCAGAATTG
  TTTTGCTCTCATTCCTAAAGCTCCCAGAAAAGATGTCGTTAAAATGCTGATGAATGACAATAAGGTGCTC
  CGCTACCTGGCGGCCCTGGAATCCCCTATCCCAGAAGACAAAGACCGTCGGTTTGTCTTCTCTTATTTTC
  TTGCCACGGATATGATCAGTATCTTTGAGCCACCTGTTCGAAATTCTGGGATCATCGGGGGCAAATTCCT
  GGGCAGGACTAAAGTTGTTAAATCATTCTCTCCAGTTGACAACCCCATCTACTACTCACCAAGTGACTTC
  TTCATCGGTGCCGTGATTGAGGTGTTCGGCCATCGGTTCGTCATCCTCGATACAGATGAGTATGTTTTAA
  AATACATGGAGAGCAACGCTTCCCAGTATTCACCAGAAGCCCTGGCCTCAATTCAGAACCGGATCCAGAA
  GCCGGAGCTTCCCGCACCAGAACTGGAAAGCAAGCAAGCTACAGGAGAACCCATGGTGCAGGGTACAGAG
  GAATCCAAGGTGCAGGACTTGGATGCACTGATAGACCAAATCCATATGCACCTGAAATACAATTCATGCA
  AAGAAAACCTCCGTGAAACATTTCAAATGTATGACAAGGATGAATCGGGATATGTGGACAGAGAGACGTT
  CTTTAAAATCTGTGAAACTCTTAATGTCCCGGTCGATGACTCGTTGATTAAGGAGTTGATCAGGCTGTGC
  ACCCACGGAGAAGGCAGGATTAACTACTATAACTTTGTTCGAGCTTTCTCGAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027974
• Insert Size: 1947 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027974.1, NP_082250.1
• RefSeq Size: 2208 bp
• RefSeq ORF: 1947 bp
• Locus ID: 71877
• UniProt ID: Q9D9T8
• Cytogenetics: 1 A4
• Gene Summary: Microtubule-associated protein which regulates cell division and neuronal migration during cortical development. Necessary for mitotic spindle organization. Necessary for radial and tangential cell migration during brain development, possibly acting as a regulator of cell morphology and process formation during migration (By similarity). May enhance calcium influx through CACNA1E and stimulate programmed cell death. Overexpression of EFHC1 in hippocampal primary culture neurons induced apoptosis.[UniProtKB/Swiss-Prot Function]