Foxk2 (NM_001080932) Mouse Untagged Clone

CAT#: MC220094

Foxk2 (untagged) - Mouse forkhead box K2 (Foxk2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Foxk2
• Synonyms: 1110054H05Rik; 5730434B08Rik; 6230415M23Rik; ILF; Ilf1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220094 representing NM_001080932
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGCGGCGGCGCTCTCGGGCGCGGGCGCCCCTCCCGCGGGCGGCGGGGCCGGGGGCGGCGGAT
  CCCCGCCGGGCGGCTGGGCCGTGGCGCGGCTCGAAGGCCGCGAGTTCGAGTACCTCATGAAGAAGCGGTC
  GGTGACCATCGGCCGCAACTCATCGCAGGGCTCGGTAGATGTGAGCATGGGCCACTCGAGCTTCATCTCG
  CGGCGCCACCTGGAGATCTTCACGCCCCCGGGCGGCGGCCATAGCGCGGCCGCCCCGGAACCCGCGCAGC
  CCCGGCCGGACGCGGGCGGCGACTTCTACCTGCGCTGTCTCGGCAAGAACGGCGTGTTCGTGGACGGGGT
  GTTCCAGCGGCGGGGGGCGCCGCCGCTGCAGCTGCCGCGCGTGTGCACTTTCAGGTTTCCGAGCACAAAT
  ATCAAGATAACATTCACAGCCCTATCCAGCGAGAAGAGAGAGAAGCAGGAAGCACCTGAGTCGCCAGTGA
  AGCCTGTGCAGCCACACATCTCACCCCTGACCATCAACATTCCAGACACGATGGCCCACCTCATCAGCCC
  CCTTCCTTCCCCCACGGGAACTATCAGTGCTGCCAACTCCTGCCCATCCAGTCCTCGCGGAGCAGGGTCT
  TCAGGGTACAAAGTGGGCCGTGTGATGCCATCGGACCTCAGTTTAATGGCTGACAACTCCCAGCCAGAAA
  ATGAAAAGGAAGCGTCAGGTGGAGACAGCCCAAAGGATGACTCAAAGCCACCTTACTCCTATGCACAGTT
  GATAGTACAAGCAATTACAATGGCTCCTGATAAACAACTTACCCTAAATGGGATTTATACACACATCACT
  AAAAACTATCCATACTACAGGACTGCGGACAAGGGCTGGCAGAATTCAATTCGCCACAATCTCTCTCTGA
  ACCGTTATTTCATCAAAGTGCCACGTTCCCAGGAAGAACCAGGCAAAGGCTCCTTCTGGAGGATAGACCC
  TGCCTCCGAGAGCAAGCTGGTAGAGCAGGCTTTTAGGAAAAGACGGCCTAGGGGCGTGCCTTGCTTTAGA
  ACCCCCCTGGGACCTCTCTCCTCTAGGAGTGCCCCAGCTTCTCCCAACCATGCCGGAGTGCTGTCTGCTC
  ACTCCAGTGGCGCCCAGACCCCCGAGAGCCTGTCGAGGGAGGGCTCCCCAGCCCCCTTGGAGCCTGAGCC
  TGGCGCCTCACAGCCCAAGCTTGCCGTCATCCAGGAGGCCAGGTTTGCCCAGAGTGCACCAGGGTCACCT
  CTCTCTAGTCAGCCCGTCTTAATTACTGTCCAGCGGCAGCTACCCCCGGCCATTAAGCCTGTCACCTACA
  CTGTTGCAACCCCAGTGACCACACCCACCTCTCAGCCACCTGTTGTGCAGACGGTCCATGTTGTTCATCA
  GATACCAGCAGTGTCAGTCACCAGTGTGGCTGGACTGGCCCCAGCAAACACATACACAGTTGCTGGACAA
  GCTGTGGTCACTCAGGCTGCGGTGCTGGCTCCTCCTAACCCTGAACCACAAGAGAATGGCGACCACAGAG
  AAGTCAGAGTGAAAGTAGAACCTGTCCCTGCAATTAGCCCAGCTACACTAGGCGCTGCTAGCCGAATCAT
  CCAGACATCACAGGGCACCCCTGTTCAGACAGTGACCATAGTGCAGCAGGCGCCTTTAGGTCAACACCAG
  CTTCCAATAAAAACTGTAACGCAAAACGGAGCTCATGTGGTACCCATGCCTACAGCAGTGCACAGCCAGG
  TGAACAATGCAGCAGCAAGTCCTCTCCATATGTTGGCCACCCATGCATCAGCATCGGCATCCCTGCCCAC
  AAAGCGCCAGAACGGTGACCAGGCAGAGCAGCCAGAGCTCAAGCGAGTTAAAGCAGAAGATGGCGAGAGC
  ATTGTCATTGCCCTGAGTGTGGATGCACCACCAGCAGCTGTGAGAGAAAAGGCAATCCAGAACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001080932
• Insert Size: 1956 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080932.2, NP_001074401.2
• RefSeq Size: 5032 bp
• RefSeq ORF: 1956 bp
• Locus ID: 68837
• UniProt ID: Q3UCQ1
• Cytogenetics: 11 E2
• Gene Summary: Transcriptional regulator involved in different processes such as glucose metabolism, aerobic glycolysis and autophagy (PubMed:25402684, PubMed:29861159, PubMed:30700909). Recognizes and binds the forkhead DNA sequence motif (5'-GTAAACA-3') and can both act as a transcription activator or repressor, depending on the context (PubMed:25402684, PubMed:29861159, PubMed:30700909). Together with FOXK1, acts as a key regulator of metabolic reprogramming towards aerobic glycolysis, a process in which glucose is converted to lactate in the presence of oxygen (PubMed:30700909). Acts by promoting expression of enzymes for glycolysis (such as hexokinase-2 (HK2), phosphofructokinase, pyruvate kinase (PKLR) and lactate dehydrogenase), while suppressing further oxidation of pyruvate in the mitochondria by up-regulating pyruvate dehydrogenase kinases PDK1 and PDK4 (PubMed:30700909). Probably plays a role in gluconeogenesis during overnight fasting, when lactate from white adipose tissue and muscle is the main substrate (PubMed:30700909). Together with FOXK1, acts as a negative regulator of autophagy in skeletal muscle: in response to starvation, enters the nucleus, binds the promoters of autophagy genes and represses their expression, preventing proteolysis of skeletal muscle proteins (PubMed:25402684). In addition to the 5'-GTAAACA-3' DNA motif, also binds the 5'-TGANTCA-3' palindromic DNA motif, and co-associates with JUN/AP-1 to activate transcription (By similarity). Also able to bind to a minimal DNA heteroduplex containing a G/T-mismatch with 5'-TRT[G/T]NB-3' sequence (By similarity). Binds to NFAT-like motifs (purine-rich) in the IL2 promoter (By similarity). Positively regulates WNT/beta-catenin signaling by translocating DVL proteins into the nucleus (By similarity).[UniProtKB/Swiss-Prot Function]