Arhgef7 (NM_017402) Mouse Untagged Clone

CAT#: MC220088

Arhgef7 (untagged) - Mouse Rho guanine nucleotide exchange factor (GEF7) (Arhgef7), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arhgef7
• Synonyms: betaPix; betaPix-b; betaPix-c; Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220088 representing NM_017402
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGATAACACCAACAGCCAACTGGTAGTACGAGCCAAGTTTAACTTCCAGCAGACCAATGAAGATG
  AACTCTCCTTCTCAAAGGGTGATGTCATCCATGTCACACGAGTGGAGGAAGGAGGCTGGTGGGAGGGCAC
  ACACAATGGCAGGACCGGCTGGTTCCCCAGCAACTACGTTCGAGAGATCAAGCCAAGTGAGAAGCCCGTG
  TCACCCAAATCAGGGACCTTGAAGAGCCCTCCCAAAGGGTTCGATACGACTGCCATCAACAAGAGCTATT
  ACAACGTGGTGCTACAGAACATCCTGGAAACAGAGCATGAGTATTCCAAGGAGCTGCAGTCTGTGCTGTC
  CACCTACCTGCGGCCACTGCAGACCAGTGACAAGTTGAGTTCAGCAAACACTTCATATTTAATGGGAAAT
  CTAGAGGAAATATCTTCCTTCCAGCAAGTGCTTGTACAGTCCCTAGAAGAATGCACGAAGTCTCCTGAAG
  CCCAACAGAGAGTTGGTGGCTGCTTCCTGAGCCTGATGCCGCAGATGAGGACCCTGTACCTCGCTTACTG
  TGCCAACCACCCATCTGCTGTGAGCGTCCTCACAGAGCACAGTGAGGACCTAGGAGAGTTCATGGAAACA
  AAAGGTGCCAGCAGCCCTGGGATCCTGGTGCTGACCACCGGCCTGAGCAAGCCCTTCATGCGCCTGGACA
  AGTACCCCACACTGCTAAAGGAGCTGGAGAGACACATGGAGGATTATCATCCTGATAGACAAGATATTCA
  GAAGTCTATGACGGCCTTCAAAAACCTTTCAGCTCAGTGTCAAGAAGTTCGCAAGAGGAAGGAGCTGGAG
  CTGCAGATCCTGACGGAGCCCATCAGGAGCTGGGAGGGGGATGACATAAAGACCCTGGGCAGTGTCACAT
  ACATGTCCCAAGTCACCATTCAGTGTGCGGGAAGCGAGGAGAAGAATGAGAGATACCTCCTGCTCTTCCC
  AAACCTTCTGCTCATGTTGTCTGCAAGTCCCAGGATGAGTGGTTTCATCTATCAGGGGAAGCTGCCAACA
  ACAGGAATGACAATCACAAAGCTTGAGGACAGTGAAAACCATAGGAATGCATTTGAGATATCAGGGAGCA
  TGATCGAGCGGATTCTGGTGTCCTGCACCAGCCAGCAGGACTTACACGAGTGGGTGGAACACCTGCAGAA
  GCAGACGAAGGTCACATCTGTGAGCAACCCCACCATCAAACCCCACTCGGTGCCATCACACACACTTCCT
  TCCCATCCTCTCACTCCATCCAGCAAACACGCGGACAGCAAGCCCGTGGCACTGACGCCTGCGTACCACA
  CACTCCCCCACCCCTCTCACCATGGCACCCCACACACCACCATCAGCTGGGGACCCCTGGAGCCTCCGAA
  GACCCCCAAGCCTTGGAGCCTGAGTTGCCTGCGGCCTGCACCTCCCCTCCGGCCCTCAGCTGCTCTCTGC
  TACAAGGAGGATCTCAGTAAGAGCCCCAAGACCATGAAAAAGCTGCTGCCGAAGCGCAAGCCCGAGCGGA
  AGCCTTCGGACGAGGAGTTCGCTGTGCGCAAGAGCACAGCGGCGCTGGAAGAAGACGCTCAGATCCTGAA
  GGTTATCGAAGCTTATTGCACAAGTGCAAAGACGCGCCAGACCCTGAACTCAAGTTCACGCAAAGAATCT
  GCTCCACAAGTGTTGCTTCCAGAAGAAGAAAAAATTATAGTCGAAGAAACCAAGAGCAATGGGCAGACAG
  TGATAGAGGAAAAGAGCCTCGTGGATACGGTGTATGCGTTAAAGGATGAAGTCCAGGAGTTAAGACAGGA
  TAACAAGAAGATGAAGAAGTCCTTAGAGGAGGAACAGAGGGCCCGCAAGGACTTAGAGAAGCTGGTGAGG
  AAGGTTCTAAAGAATATGAACGACCCTGCCTGGGATGAGACCAATCTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_017402
• Insert Size: 1941 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017402.4, NP_059098.2
• RefSeq Size: 4488 bp
• RefSeq ORF: 1941 bp
• Locus ID: 54126
• UniProt ID: Q9ES28
• Cytogenetics: 8 A1.1
• Gene Summary: Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. May function as a positive regulator of apoptosis. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR and contains multiple coding differences including translation initiation at a downstream start codon, compared to variant 1. The resulting isoform (c) is shorter than isoform a.