Pum3 (NM_177474) Mouse Untagged Clone

CAT#: MC220086

D19Bwg1357e (untagged) - Mouse DNA segment, Chr 19, Brigham & Women's Genetics 1357 expressed (D19Bwg1357e), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pum3
• Synonyms: 1110069H02Rik; AA675048; D19Bwg1357e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220086 representing NM_177474
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAAGTCAAAGGGAAGAAAAAGTTTACAGGAAAGAGTCCACAGACATCACAAGGAAAGAATAAAT
  TTCATAAGAACAGTGAGTCTAGTTCTTCAAAGACATTCCCAAGGAAAGCTGTTAAGGAAGGCGGACCTAA
  AGTCACATCTAAGAACTTTGAGAAAGGTGCCACAAAACCTGGCAAGAAAGGTGTGAAGCAGTTCAAGAAC
  AAGCCACAAGGGGGCAAAGGACCGAAGGACAAATTTCAGAAGGCAAATAAATTCAGCAAGAAGCGGAAAT
  TCCAGCCAGATGGTGAAAGTGATGAATCAGGAGCCAAGAAACCCAAGTGGGATGACTTCAAAAAGAAGAA
  GAAAGAGCTGAAGCAGAGCAGGCAGCTCAGTGACAAGACCAACTATGACATCGTGGTTCGAGCAAAGCAC
  ATTTGGGAGAGCTTGAGAAGAAAAGATTGTGACAAGGAAAAACGGGTGAAGCTCATGAGTGATTTGCAGA
  AGTTGATTCAAGGGAAAATTAAAACTATCGCCTTTGCACATGACTCGACGCGCGTGATCCAGTGCTTCAT
  TCAGTATGGGAACGAGGAGCAGAGGAAGCAGGCTTTCCAAGAGTTGCAAGGCGACTTGGTTGAATTAAGT
  AAAGCCAAATATTCCAGGAATATCGTTAAGAAATTTCTCATGTATGGAAGTAAGCCACAAGTTGCGGAGA
  TAATCAGAAGTTTTAAAGGTCATGTGAGGAAGATGCTGCGGCATTCGGAGGCATCGGCCATTGTGGAGTA
  CGCATACAACGACAAAGCCATTTTGGAACAGAGGAACATGCTGACAGAGGAGCTCTATGGGAACACGTTT
  CAGCTTTACAAGTCAGCAGATCACCCAACGCTGGACAAGGTGCTGGAGCTGCAGCCAGCAAAGCTAGAGC
  TTATCATGGACGAGATGAAGCAGATTCTGACACCGATGGCCCAAAAGGAAGCTGTGATTAAGCACTCACT
  CGTTCATAAAGTATTCTTGGACTTTTTTACCTACGCACCCCCAAAACCTCGATCAGAACTAATTGAAGCC
  ATCCGGGAAGCAGTGGTGTACCTGGCCCACACACACGATGGCGCCAGAGTGGCCATGCACTGCCTGTGGC
  ACGGCACACCCAAGGACAGGAAAGTGATCGTGAAAACGATGAAGACGTATGTGGAGAAGGTTGCCAATGG
  CCAGTACTCTCACTTGGTTCTCCTGGCAGCGTTTGACTGTATTGATGATACTAAGCTTGTGAAGCAGATA
  ATCATATCAGAAATCATCAGTTCCTTGCCCAGCATAGTTAATGACAAATACGGAAGGAAAGTCCTCTTGT
  ACTTGATGAGCCCCAGAGACCCCGCCCACACGGTGCCCGAGCTCATCGAGCTTCTGCAGAAGGGTGATGG
  CAACGCGCACAGCAAGAAGGACACTGCAATCCGCCGGCGTGAGCTCTTGGAGTCCATCTCTCCAGCTTTG
  CTGAGCTACCTGCAAGGACACACTCAAGAGGTGGTGCTGGATAAGTCTGCATGTGTGCTGGTGTCTGACA
  TGCTGGGATCTGCTACTGGAGATGTTCAGCCTGCTATGGATGCCATCGCCAGTTTGGCAGCAGCAGAACT
  GCATCCTGGTGGCAAGGACGGAGAGCTTCATGTTGCTGAGCATCCTGCAGGACATCTGGTTCTCAAATGG
  TTACTAGAGCAAGATAAAAAGATGAAAGAAAGCGGAAAGGAAGGTTGTTTTGCAAAAACCCTTGTAGAGC
  GTGTTGGTATGAAGAACCTCAAGTCCTGGGCCAGCATCAACCGAGGTGCCATTATTCTTTCCAGCCTTCT
  TCAGAGTTGTGACCAAGAAGTTGTAAACAAAGTCAAAGGTGGACTGAAACCCCTGATTCCTACCTTGGAA
  AAAAACAAAAGCAGCAGCAGAGGCATCCAGACCCTGCTTGAGAAGCTGACTGCGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177474
• Insert Size: 1947 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177474.5, NP_803425.1
• RefSeq Size: 3596 bp
• RefSeq ORF: 1947 bp
• Locus ID: 52874
• UniProt ID: Q8BKS9
• Cytogenetics: 19 21.96 cM
• Gene Summary: This gene encodes a member of the evolutionarily conserved Pumilio and Fem-3 mRNA-binding factor family of proteins, which are characterized by tandem 36 amino acid pumilio homolog domains and which function in diverse biological processes. This protein belongs to a group of atypical Pumilio and Fem-3 mRNA-binding factor proteins, whose members are distinguished from other Pumilio and Fem-3 mRNA-binding factor proteins by a novel protein fold with 11 pumilio homolog domains and an ability to bind to DNA and single- and double-stranded RNA without sequence specificity. In mouse, lower levels of gene expression have been correlated with increased testicular germ cell tumors. A pseudogene of this gene is found on chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the protein.