Tcf3 (NM_001164153) Mouse Untagged Clone

CAT#: MC220076

Tcf3 (untagged) - Mouse transcription factor 3 (Tcf3), transcript variant 7, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tcf3
• Synonyms: A1; AA408400; ALF2; AW209082; bHLHb21; E2A; E12; E12/E47; E47; KA1; ME2; Pan1; Pan2; TCF-3; Tcfe2a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220076 representing NM_001164153
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAACCAGTCTCAGAGAATGGCACCCGTGGGCTCTGACAAGGAACTGAGTGACCTCCTGGACTTCA
  GCATGATGTTCCCGCTACCTGTGGCCAATGGGAAGAGCCGGCCCGCCTCCCTCGGGGGAACCCAGTTTGC
  AGGCTCAGGACTGGAGGACCGACCCAGCTCAGGCTCCTGGGGCAGCAGTGACCAGAACAGTTCTTCCTTT
  GACCCTAGCCGGACATACAGCGAAGGTGCCCACTTCAGTGACTCCCACAGCAGCCTGCCGCCTTCCACGT
  TCCTAGGAGCTGGGCTTGGAGGCAAGGGCAGTGAGCGGAATGCCTATGCCACCTTTGGGAGAGACACCAG
  TGTTGGCACCTTGAGTCAGGCTGGCTTCCTGCCAGGTGAGCTGAGCCTCAGCAGTCCCGGGCCACTGTCC
  CCATCGGGCATCAAGAGCAGCTCCCAGTATTACCCCTCATTCCCCAGCAACCCTCGTCGGAGAGCTGCAG
  ATGGTGGCCTGGATACTCAGCCGAAGAAGGTCCGGAAGGTTCCGCCTGGTCTCCCTTCCTCGGTGTATCC
  GCCCAGCTCAGGTGACAGCTACAGCAGGGATGCTGCAGCCTACCCCTCCGCCAAGACCCCCAGCAGCGCT
  TACCCCTCCCCCTTCTACGTGGCAGATGGCAGCCTGCACCCATCAGCTGAGCTCTGGAGTACGCCTAGCC
  AGGTGGGCTTTGGGCCCATGCTAGGTGACGGCTCTTCCCCTCTGCCCCTTGCACCGGGCAGCAGCTCCGT
  GGGCAGTGGTACCTTTGGGGGCCTCCAGCAGCAGGATCGCATGGGCTACCAGCTGCATGGATCTGAGGTT
  AATGGCTCGCTCCCAGCTGTATCCAGCTTTTCGGCTGCCCCTGGCACTTACAGTGGGACTTCCGGCCACA
  CGCCCCCTGTGAGTGGGGCCGCAGCTGAAAGCCTCCTAGGCACCCGAGGGACTACAGCCAGCAGCTCAGG
  GGATGCCCTTGGGAAGGCACTGGCCTCGATCTACTCCCCGGATCACTCCAGCAATAATTTCTCACCTAGC
  CCCTCAACGCCTGTGGGTTCACCCCAGGGCCTGCCAGGGACATCACAGTGGCCCCGGGCAGGAGCGCCCA
  GTGCCTTATCCCCCAACTACGATGCAGGTCTCCATGGCCTGCAGAGCAAGATGGAGGACCGCTTGGACGA
  GGCCATCCATGTCCTGCGAAGCCACGCTGTTGGCACCGCTAGCGATCTCCATGGGCTTTTGCCTGGCCAT
  GGCGCACTGACCACGAGCTTCACCGGCCCCATGTCACTGGGCGGGCGGCATGCCGGCCTGGTCGGGGGAA
  GCCATCCTGAGGAGGGCCTCACAAGTGGGGCCAGTCTTTTGCATAACCATGCCAGCCTCCCCAGCCAGCC
  CAGTTCCCTCCCTGACCTCTCACAGAGACCTCCCGACTCCTATAGTGGACTCGGGAGGGCAGGCACAACA
  GCGGGTGCCAGCGAGATCAAGCGGGAGGAGAAAGAGGATGAGGAAATCGCATCAGTAGCCGACGCCGAAG
  AGGACAAGAAGGACCTGAAGGTCCCACGCACGCGCACCAGTACAGATGAGGTGCTGTCCCTGGAGGAGAA
  GGACCTGAGGGACCGGGAGAGGCGTATGGCCAATAACGCTCGGGAGCGGGTGCGCGTGCGGGACATTAAC
  GAGGCCTTCCGGGAGCTGGGCCGCATGTGCCAGCTGCACCTCAAGTCGGATAAGGCGCAGACCAAGCTGC
  TCATCCTGCAGCAGGCGGTGCAGGTCATCCTGGGCCTGGAGCAGCAGGTGCGAGAACGCAACCTGAACCC
  CAAAGCAGCCTGCTTGAAGCGGAGGGAGGAGGAGAAGGTGTCTGGCGTGGTCGGGGACCCACAGCTGGCC
  CTGTCAGCCGCCCACCCGGGCCTGGGTGAGGCCCACAACCCAGCCGGGCACCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164153
• Insert Size: 1947 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164153.1, NP_001157625.1
• RefSeq Size: 3305 bp
• RefSeq ORF: 1947 bp
• Locus ID: 21423
• Cytogenetics: 10 39.72 cM
• Gene Summary: Transcriptional regulator. Involved in the initiation of neuronal differentiation. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (7) has an alternate splice site and an alternate penultimate coding exon, compared to variant 1. The resulting isoform (7) lacks an internal aa and has a distinct segment in the C-terminal region, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.