Lrwd1 (NM_027891) Mouse Untagged Clone

CAT#: MC220056

Lrwd1 (untagged) - Mouse leucine-rich repeats and WD repeat domain containing 1 (Lrwd1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lrwd1
• Synonyms: 1200011O22Rik; AU042569; AW548074; Orca
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220056 representing NM_027891
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCTCTCACGCCGCAACTGCTCCTGCAACGTGGGCGACCCAAGACGGATAAGCTGGGGAAGATTC
  AGAGTTTGAATCTGTCGGGGCTGCAGCTGCTCTCAGAGCACTTGGATCCCAATCTCCTGGGGCGCCTGAA
  GAAGCTGAGGGAACTTGACCTCTCTAACAACTTGCTGGAGACGCTGCCTGCTAACCTGGGCCTGTCCCAT
  CTGCGCATCCTTCGCTGTACCAACAACCAGCTCGGGGATGTCACTGCCTTGCACCAGTTCCCAGAACTTG
  AGGAGCTCAATCTGGAAGGCAACCCCTTCCTGACGGTCAGTGACAACCTCAAAGTCTCCTTTCTCCTGCC
  CAAGCTTCGTAAGGTGAATGGCAAGGACACGGCCTCCACCTGCTCACAGGTGGAGAATCTAGATCGGGAG
  CTGATGGACAGGGTCACAGCTCACTGGCAGAAGTTCATAGCCACTGTGAGCCCTGAGGAGGAAACTGACA
  AGGTGCGGGCTGATTTCATGAGGTCTGCAGTCAGGGATGTGTGTTACGGCCCTGAGTCGCTCATCGAGTT
  CACGCAGTGGCGGGTGCGGATGATCGCCGAGGAACTGGTGGCCTCAGGTGGGGCCCAGGTGCAAGACGCT
  AAGGTCCCCGTGGAGCATCCCCAGGCTGCAGGTGCCTCCAAGTTCAGGGCCAGAGAGGTGGCTTCAAAAC
  GGCCAGGTAAAGACCCAGTCACCCTTCCTCCCAGCAAGCGAGTGCGTGCTCTCCCACCTGCTCAGGCTGA
  GGGCAGTCCTATGGGTGCTGATGGAGGGCAGGCTGCCCTACACCTGGAACCCCTGCACTTCCTGCAGTGT
  CATAGCAGAAACAACAGCCCAAAGGACCTGGAGACCCAGCTGTGGGCCTGTGCCTTTGAGCCGGCCCGGG
  AGGAGGGGCACTCGAGGGCCACATCCCAGACCGTGGCCACATGTGGTGGAGAGGCTGTTTGCGTGATAGA
  CTGCCAGACAGGCCTCGTACTCCATAAGTACAAGGTACCAGGCGAGGAGTTCTTCTCAGTGGCCTGGACA
  GCCCTCACGGTGGTCACCCAGGCAGGCCACAAGAAGCGCTGGAACATGCTAGCAGCTGCAGGCCTCCGCG
  GCATGGTCCGGCTGCTGCATGTTCGCGCAGGCTTCTGCTGCAGCGTCATCCGGGCCCATAAGAAGGCCAT
  TGCCACCCTCTGCTTCAGCCCCAGCCATGAGACTCACCTCTTCACTGCCTCCTACGACAAACGGATCATC
  CTCTGGGACATTGGGGTGCCCAACCAGGATTACAAGTTCCAGGCTAGCCAGCTCCTCACGCTCAACTGTG
  GTTCTGTCCCCTTGCGCCTCTGTCCTGTTGCCACCTGCCCAGATGACTTCCTGCTGGCTGGCTGTGAGGG
  TGGCTGCTACTGCTGGGACGTGCGGCTAGACCAGCCCCAGAAGCAAAGGGTGTGTGAGGTGAACTTCATC
  TTCTCTGAGGACTCCAAGGTGTCAGGACAAAGAGTCGATGGGCTGGCATTTGTGAATGAAGATGTGGTGG
  CCTCCAAAGGGAGTGGACAGGGCACCATCTACCTGTGGAGCTGGAGCCAGACATGGGCAGGCCGGGGCAG
  ACAGTCTGTGTTGCCCGTAGTCATCCTGGTCCGGCTGCAGTGGTCACCCACCAACCTGGCCTACTTTTCA
  CTCAGCACCTGTCCCGGCAAAAACCTCGTGCTGTGTGGGGACGAGGAAGGCAGTGTGTGGATCTATGATG
  TTGAACACCTCCTGAAAGAGCCTCCACAGGCAACAACTCTGCAGCCCCCAACACAGATCCTGAAGTGGCC
  TCAGCCAACAGCATTGGGCCAACCAGTGACCAAGACCATGATAAACACAGTCGTGGCCAATGCAGCCTTC
  ACCTACCTCACTGCCCTGACAGACTCCAACATCGTGTCCATCTGGAGGAGATGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027891
• Insert Size: 1947 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027891.4, NP_082167.2
• RefSeq Size: 2201 bp
• RefSeq ORF: 1947 bp
• Locus ID: 71735
• UniProt ID: Q8BUI3
• Cytogenetics: 5 G2
• Gene Summary: Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation (By similarity). Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability.[UniProtKB/Swiss-Prot Function]